聚合酶链反应诊断女性外阴尖锐湿疣初步探讨

聚合酶链反应诊断女性外阴尖锐湿疣初步探讨郑惠方单祥年李明发蒋清龚嘉仪（南京铁道医学院附属医院妇产科，生物学教研室，附属医院病理科南京２１０００９）尖锐湿疣（ＣＡ）主要由人乳头瘤病毒（ＨＰＶ）６及１１型ＤＮＡ感染引起。尖锐湿疣和假性湿疣...     

风险动态评估及预防性护理对住院患者压力性损伤发生率的影响

目的：探讨风险动态评估与预防性护理对住院患者压力性损伤(PI)发生率的干预效果。方法：选取江西省高安市人民医院2020年1—7月收治的32例有PI风险的患者作为研究组,给予预防性护理干预;按照1∶1的比例选取2019年6—12月收治的32例有PI风险的患者作为对照组,予以常规护理。干预2周后,比较两组患者压力性损伤发生情况及健康状况调查量表(SF-36)评分。结果：干预后,研究组PI总发生率为6.25%,低于对照组的25.00%,差异有统计学意义(χ²=4.267,P<0.05)。干预前两组患者SF-36各维度得分比较,差异无统计学意义(t=1.240,0.597,1.159,0.105,0.673,0.647,0.707,0.434,P>0.05);干预后,研究组SF-36各维度得分均高于对照组,差异有统计学意义(t=6.993,4.754,10.396,4.330,2.317,2.810,2.511,3.758,P<0.05)。结论：风险动态评估及预防性护理可有效控制住院患者PI的发生,提高患者机体健康水平与生活质量。     

Flow-induced Expression and Phosphorylation of VASPin Endothelial Cells

1 Introduction It is well known that mechanical forces have important influence on endothelial cells, in particular, on cytoskeleton reorganization. VASP (vasodilator stimulated phosphoprotein) is a 46 KD actin associated protein. It is a member of Ena/VASP protein family and composed of EVH1, proline-rich and EVH2 domains. It is considered as an important component of the sub-cellular regions where remodelling of the actin cytoskeleton takes place, such as the front of spreading lamell…     

Chronic neuroprotective effects of low concentration lithium on SH-SY5Y cells:possible involvement of stress proteins and gene expression

To investigate the molecular mechanism underlying the neuroprotective effect of lithium on cells, in this study, we exposed SH-SY5Y cells to 0.5 mmol/L lithium carbonate(Li2CO2) for 25–50 weeks and then detected the expression levels of some neurobiology related genes and post-translational modifications of stress proteins in SH-SY5Y cells. cDNA arrays showed that pyruvate kinase 2(PKM2) and calmodulin 3(CaM 3) expression levels were significantly down-regulated, phosphatase protein PP2A expression was lightly down-regulated, and casein kinase II(CK2), threonine/tyrosine phosphatase 7(PYST2), and dopamine beta-hydroxylase(DBH) expression levels were significantly up-regulated. Besides, western blot analysis of stress proteins(HSP27, HSP70, GRP78 and GRP94) showed an over-expression of two proteins: a 105 kDa protein which is a hyper-phosphorylated isoform of GRP94, and a 108 kDa protein which is a phosphorylated tetramer of HSP27. These results suggest that the neuroprotective effects of lithium are likely related to gene expressions and post-translational modifications of proteins cited above.     

百令胶囊对频繁发作 ＣＯＰＤ 病人免疫功能和肺功能的影响

目的：观察百令胶囊对频繁发作慢性阻塞性肺疾病（ＣＯＰＤ）病人免疫功能和肺功能的影响。方法： 选取 １００ 例年发作次数在 ２ 次以上，每次发作均来本院呼吸内科住院诊治的慢性阻塞性肺疾病急性加重期病人 （ＡＥＣＯＰＤ）。５２ 例对照组病人在常规治疗的基础上增加无创正压通气（ＮＩＰＰＶ），４８ 例观察组病人在对照组之 上增加百令胶囊治疗。观察两组病人治疗前后免疫功能和肺功能情况。结果：观察组临床治疗有效率为８３．３３％ 显著高于对照组的６５．３８％，有统计学意义（χ２ ＝ ４．１８，Ｐ＜０．０５）；观察组治疗后 ＦＥＶ１ 、ＦＥＶ１ 预测值、ＦＥＶ１ ／ ＦＶＣ 均 高于对照组，差异有统计学意义（Ｐ＜０．０５）；观察组治疗后 Ｔ 淋巴细胞亚群 ＣＤ４＋、ＣＤ４＋ ／ ＣＤ８＋高于对照组，ＣＤ８ ＋低于对照组，均有统计学差异（Ｐ＜０．０５）。结论：采用百令胶囊辅助治疗，可改善病人免疫功能，提高肺功能，有 望降低 ＡＥＣＯＰＤ 发作次数。     

利多卡因喷雾剂和麻黄碱在30例经鼻插管洗胃术中的应用

目的:探讨利多卡因喷雾剂和麻黄碱在经鼻插管洗胃术中联合应用的效果,旨在提高护理人员的专业技能,为病人提供安全、及时、有效的护理操作技术。方法:随机选择60例农药中毒的病人进行研究,把病人分为试验组和常规组,分别采用不同的插管方法实施插胃管术,观察两种方法的应用效果。结论:经鼻插管洗胃术前联合应用利多卡因喷雾剂和麻黄碱,可大大地提高插管的成功率,减轻病人的痛苦及并发症的发生。     

正确和完整解读《甲乙经》的学术思想——《甲乙经》析疑五则

《甲乙经》一书,乃《针灸甲乙经》之简称,为晋代皇甫谧据《素问》、《针经》、《针灸治要》三书内容除去重复重新归类编撰而成,是针灸学的一部宝贵典籍。由于年代久远,文字错简脱落者有之,亥豕鲁鱼者有之,字形义训变迁者亦有之,今人一般很难通读之,必须采用研究古籍方法始克奏效。     

从肝论治疑难病案分析

患者张某,男,44岁,1980年5月22日门诊。 据患者述1960年感右胁隐痛,疑为肝炎,而作超声波、肝功能检查,结果均无异常发现。右胁隐痛持续至1965年5月,每日痛2～3次,有时可间歇半个月不痛,痛位不甚固定,并连及右腰背,当时又疑为胆囊炎,在某院住院检查,行胆囊造影及。肾脏造影均无异常。     

Interleukin 28B-related polymorphisms: A pathway for understanding hepatitis C virus infection?

AIM:To analyze the role of rs12979860 and rs8099917polymorphisms in hepatitis C virus(HCV)genotype 1infection of Brazilians.METHODS:A total of 145 adult patients diagnosed with genotype 1 chronic hepatitis C(CHC)who had completed a 48-wk regimen of pegylated-interferonα-2a or-2b plus ribavirin combination therapy were recruited from six large urban healthcare centers and199 healthy blood donors(controls)from a single site between January 2010 and January 2012.Data on the patients’response to treatment was collected.Polymerase chain reaction-restriction fragment length polymorphism genotyping of the interleukin(IL)28B gene fragment encompassing the single nucleotide polymorphisms(SNPs)rs12979860(C/T)and rs8099917(T/G)was carried out for 79 of the CHC patients and 199 of the controls.Bi-directional amplicon sequencing of the two SNPs was carried out for the remaining 66 CHC patients.RESULTS:SNP rs12979860 genotyping was successful in 99.5%of the controls and 97.2%of the CHC patients,whereas the SNP rs8099917 genotyping was successful in 95.5%of the controls and 100%of the CHC patients.The genotype and allele distributions for both rs12979860 and rs8099917 were significantly different between the control and CHC patient groups,with significantly higher genotype frequencies of CC and TT in the controls(P=0.037 and 0.046,respectively)and of TT and GG in the CHC patients(P=0.0009and 0.0001,respectively).Analysis of the CHC patients who achieved sustained virological response(SVR)to treatment(n=55)indicated that the rs12979860 C allele and CC genotype were predictors of SVR(P=0.02).No significant correlation was found between rs8099917 genotypes and treatment response,but carriers of the T allele showed significantly higher rates of SVR(P=0.02).Linkage disequilibrium analysis of the group that achieved SVR showed a significant association between rs12979860 and rs8099917(P=0.07).CONCLUSION:The higher allele frequency of rs12979860 C and rs8099917 T observed in non-HCVinfected individuals may indicate a potential pro     

周围神经卡压综合征影像诊断的研究进展

周围神经经过某些解剖上狭窄部位的急、慢性挤压或者反复摩擦造成局部水肿等炎症反应,使局部容积减小,血液循环障碍,造成该处甚至远处神经发生脱髓鞘改变,出现支配区不同程度的运动及感觉功能障碍的现象称之为神经卡压综合征。传统的周围神经卡压综合征的诊断主要依靠其临床表现、物理检查及电生理检测,是一种主观的判断。近年