甲醛固定对阿片受体结合能力的影响

应用放射受体分析和体外受体放射自显影技术,研究了甲醛固定对阿片受体结合能力的影响。结果表明,1％多聚甲醛溶液中孵育30分钟或12小时,对大鼠脑膜阿片受体结合能力均无显著影响; 2％或4％多聚甲醛溶液中孵育30分钟,对阿片受体结合能力也无显著影响。经甲醛固定的脑切片上的阿片受体,与[~3H]-埃托啡结合的饱和曲线与新鲜脑切片的相似。用1％多聚甲醛固定的组织切片进行体外受体放射自显影,较好地显示了阿片受体的分布。这些结果提示,甲醛固定的组织可用于进行体外受体放射自显影研究。     

血塞通注射液对沙土鼠脑缺血损伤的保护作用

通过夹闭沙土鼠双侧颈总动脉１０ ｍｉｎ 或２０ ｍｉｎ 后再灌７ ｄ 或１ ｄ , 造成脑缺血再灌模型, 检测血塞通对沙土鼠脑组织钙、水含量的影响, 以及对海马 Ｃ Ａ１ 区死亡的神经元迟发性损伤的保护作用． 结果显示, 血塞通可降低缺血后脑组织钙的含量, 减少海马 Ｃ Ａ１ 区死亡的神经元数量, 增加神经元密度． 实验结果提示血塞通对脑缺血再灌损伤有一定的保护作用．     

血清miR-206、CA724和PGⅠ联合检测在胃癌诊断和预后评估中的价值

目的探讨血清微小RNA-206(miR-206)、糖类抗原724(CA724)和胃蛋白酶原Ⅰ(PGⅠ)联合检测在胃癌诊断和预后评估中的价值。方法收集2014年8月至2015年6月该院确诊的200例胃癌患者纳入胃癌组,收集同期该院健康体检者200例纳入健康对照组,检测两组miR-206、CA724和PGⅠ水平。结果相对于健康人群,胃癌患者血清miR-206和PGⅠ水平降低,而CA724水平增高,差异均有统计学意义(P0.05)。血清PGⅠ、miR-206和CA724鉴别诊断胃癌与健康人群的敏感度/特异度分别为78.5%/86.9%、78.7%/90.7%和70.2%/59.3%;对应的临界值分别为:43.52μg/L、8.84和5.86U/mL。3项指标联合检测诊断胃癌的敏感度为92.3%,特异度为83.4%。相对于血清低miR-206、低PGⅠ与高CA724水平的患者,高miR-206、高PGⅠ与低CA724水平的患者的累积生存率提高,差异均有统计学意义(P0.05)。低miR-206、低PGⅠ与高CA724水平是胃癌发病的独立危险因素(P0.05)。结论血清miR-206、CA724和PGⅠ联合检测对胃癌具有较高的诊断及预后评估价值。     

老年人腹腔镜胆囊切除108例治疗体会

目的;探讨老年人腹腔镜胆囊切除术（LC）围手术期处理及疗效。方法;分析我院108例腹腔镜胆囊切除的老年患者治疗经过。结果：老年人多合并高血压、冠心病、糖尿病及呼吸系统疾病。如能对围手术期做出合理处理,老年患者均在术后痊愈早期出院,无1例死亡及严重并发症。结论：LC也适合于广大老年患者。     

清宫益寿养颜补酒秘方

历代宫廷生活饮食丰富,而四体不勤,心血管系统、运动系统难免会过早衰退,如何调理以延续皇帝后妃的健康,是御医们研究的最重大的课题,因此宫廷医学中集中了大量的益寿药膏,健身补酒、长寿丹丸、延年药膳等医方医案,密藏于深宫中。这些补益方药对现代人也很有参考价值。下面,择选清代宫廷益寿养颜药酒方10则,     

GIuma脱敏剂治疗牙本质敏感症的临床观察

目的：探讨ＧＩｕｍａ脱敏剂治疗牙本质敏感症的疗效。方法：观察２５例１１６颗患牙的治疗情况，并作即刻、１个月后、半年后复查观察记录。结果：即刻总有效率为８９．６％，１个月后总有效率为８０．２％，半年后总有效率为７４．１％。结论：采用ＧＩｕｍａ脱敏剂治疗牙本质敏感症，临床疗效显著，有推广意义。     

ORAL BAICALIN AND LIQUID EXTRACT OF LICORICE REDUCE SORBITOL LEVELS IN RED BLOOD CELL OF DIABETIC RATS

Intracellular accumulation of sorbitol, resulting from the increased glucose flux through polyol pathway with the action of aldose reductase, has been found pathogenic to chronic diabetic complications. It has been reported that baicalin and Glycyrrhiza uralensis Fisch can inhibit rat lens aldose reductase in vitro. In order to determine whether the said medicinal herbs give the inhibitive effect in vivo, 10 streptozotocin induced diabetic rats were treated with baicalin 150 mg/kg.d and liquid extract of licorice (LEL) 7.5 ml/kg.d for a week. RBC sorbitol was determined by a fluorometric assay before and after the treatment, as well as one week afterwards. It was found that oral baicalin and licorice dramatically reduced sorbitol levels in RBC without affecting blood glucose levels. The sorbitol levels was restored to its original values one week after discontinuing the treatment. The present study has demonstrated the effectiveness of baicalin and licorice in reducing RBC sorbitol levels in diabetic rats. Its mechanism is presumably through inhibition of aldose reductase. The feasibility of its clinical application for the control of diabetic complications is under investigation.     

腹腔镜胆囊切除中转开腹28例临床分析

腹腔镜胆囊切除术（LC）的选择在术前有很多因素决定。术中是否中转开腹，与胆囊粘连程度以及结石嵌顿的部位、大小和术者的熟练程度有很大的关系。