脊柱复合性损伤的救治风险与早期治疗

目的评估脊柱复合性损伤的特点和救治风险,探讨风险控制与最佳治疗的方法。方法采用AIS、ISS、TRISS及APACHEⅡ等评分方法对273例脊柱复合性损伤患者进行定量评价与救治分类,并依据伤后的损伤分级、参数评定及计量评分等指标进行量化分析和统计处理。结果颈椎合并伤115例,胸椎合并伤141例,胸腰椎合并伤294例,腰骶椎合并伤181例;患者的救治风险和脊椎伤的治疗选择或手术时机与其合并伤的解剖伤势及由此所构成的整体伤情密切相关(P<0.01或<0.05);高风险性伤员往往综合伤势严重,生存概率(Ps)趋低,并发症和死亡率高(P<0.01或<0.05)。结论脊柱脊髓损伤常合并有严重的多发伤,高危伤情不仅可增加其救治风险和脊柱伤的处理难度,且还易于丧失手术最佳时机。分类救治对伤员的风险控制和脊柱伤的专科治疗是有益的。     

1994～2002年广西甲型副伤寒和伤寒流行情况分析

目的 了解1994～2002年接种伤寒Vi疫苗后的7年间伤寒和副伤寒流行变化，评价Vi疫苗对控制伤寒流行的效果并分析甲型副伤寒的特征及影响其爆发流行的因素。方法对自治区疾病控制中心实验室记录、伤寒副伤寒爆发调查报告、监测系统资料、全区伤寒流行地区伤寒Vi疫苗供应情况进行统计分析。结果 经过血培养证实甲型副伤寒病例为1200例、伤寒为285例；甲型副伤寒与伤寒的流行病学、临床特征十分相似；饮用污染水是引起甲型副伤寒爆发的主要原因；从1999年起，甲型副伤寒杆菌开始超过伤寒菌为优势流行菌株。结论 在广泛使用伤寒Vi疫苗后，伤寒基本得到控制，但因为引起副伤寒发病的危险因素依然存在，甲型副伤寒已取代寒，成为广西农村地区的最重要的传染病之一。为控制甲型副伤寒的流行，在安全饮用水供应状况得到全面改善之前，应尽快研制出副伤寒疫苗。     

噻吗洛尔与普萘洛尔治疗高血压病与心绞痛的疗效比较

劳累性心绞痛38例采用噻吗洛尔2.5-5mg, tid,共4wk;另12例采用普萘洛尔10-20mg, tid,共4wk。结果:前者显效率为79％,显著高于后者42％(P<0.05)。高血压病(Ⅰ,Ⅱ期)37例采用噻吗洛尔治疗,方法同上;另13例采用普萘洛尔20-40mg, tid,疗程亦4wk。结果:2组均有显著降压疗效(P<0.01),噻吗洛尔治疗后1h即获显效,普萘洛尔须24h才获显效。噻吗洛尔7％(5/75)、普萘洛尔4％(1/25)治疗后发生窦性心动过缓,前者被迫停药,后者未停药。     

一株铜绿假单胞菌中检出两种新的整合子

目的研究铜绿假单胞菌多重耐药的临床分离株RJ217中发现的两个新整合子的结构，并分析其在多重耐药性中的作用。方法用改良三相试验分析RJ217产β-内酰胺酶的情况，用常规和长片段PCR法扩增耐药基因和整合子，并对PCR产物进行序列分析。结果发现铜绿假单胞菌RJ217携带两个新的整合子，其中一个携带veb-I型超广谱β-内酰胺酶(ESBL)基因，这两个整合子的结构分别为IS10-like-veb-I-aadB-oxa10／aadA1和aadB-oxa10／aadA1。结论整合子介导的耐药基因在RJ217的多重耐药性中发挥了重要的作用。     

Exploring cancer register data to find risk factors for recurrence of breast cancer – application of Canonical Correlation Analysis

Background  

A common approach in exploring register data is to find relationships between outcomes and predictors by using multiple regression analysis (MRA). If there is more than one outcome variable, the analysis must then be repeated, and the results combined in some arbitrary fashion. In contrast, Canonical Correlation Analysis (CCA) has the ability to analyze multiple outcomes at the same time.     

Genetic Conservation of Hemagglutinin Gene of H9 Influenza Virus in Chicken Population in Mainland China

The hemagglutinin (HA) genes of 12 H9N2 influenza virus strains isolated from chickens in Mainland China during the period 1995–2002 were genetically analyzed. All the isolates possessed the same amino acid motif -R-S-S-R/G-L- at the cleavage site of HA. Except for the conserved amino acids, as is the case in the other avian influenza viruses, located in the receptor binding site, all of the 12 isolates possessed N at amino acid position 183; A, T, or V at position 190; K at position 137, whereas the representative strains of the other lineage (except Dk/HK/Y280/97-like lineage) virus of H9N2 viruses had H, E, and R at these positions respectively. These could be considered as the partial molecular markers of the H9 viruses isolated from chickens in Mainland China. Phylogenetic analyses showed HA genes of these isolates belonged to that of A/duck/Hong Kong/Y280/97-like virus lineage. No A/quail/Hong Kong/Gl/97-like virus was found in chicken, population since the outbreak of H9N2 influenza in Mainland China in 1992. The available evidence indicates that HA genes of H9 influenza virus circulating in Mainland China during the past years were well conserved.     

Phytoremediation of Cadmium Contaminated Soil: Impacts on Morphological Traits,Proline Content and Stomata Parameters of Sweet Sorghum Seedlings

Phytoremediation is a green, simple, eco-friendly, sustainable, and cost-effective remediation technology to remove and degrade contaminants from soil. In this study, a germination experiment and a pot experiment were performed in greenhouse to evaluate cadmium toxicity and phytoremediation capacity. The results showed that there was the highest membership function value of cadmium (MFVC) in KFJT-3 than that of KFJT-CK and KFJT-1, the value being 0.473, 0.456 and 0.413, respectively. Furthermore, the highest biomass was discovered in KFJT-3 compared to the other genotypes under 50 mg/kg cadmium stress. Physiological analysis showed that proline content significantly increased in KFJT-3, the value being 31.88%. In addition, Bioaccumulation factor (BAF) and Translocation factor (TF) value were 3.80 and 1.02 for KFJT-3, respectively. In conclusion, BAF and TF values showed that the cadmium tolerance of KFJT-1 and KFJT-3 could be higher than that of KFJT-CK, which could be the genotype for phytoremediation of cadmium contaminated soil.

     

Altered impedance during pigment aggregation in<Emphasis Type="Italic">Xenopus laevis</Emphasis> melanophores

Melanophores are dark-brown pigment cells located in the skin of amphibia, fish and many invertebrates. The skin colour of these organisms is regulated by the translocation of pigment organelles, and the pigment distribution can be altered by external stimuli. The ability to change colour in response to stimuli makes these cells of interest for biosensing applications. It was investigated whether pigment aggregation in Xenopus laevis melanophores can be detected by impedance measurements performed in transparent microvials. The results show that cell attachment, cell spreading and pigment aggregation all resulted in impedance changes, seen particularly at the highest frequency tested (10 kHz). The mechanisms behind the impedance changes were investigated by the addition of latrunculin or melatonin, both of which cause pigment aggregation. The latrunculin-induced aggregation was associated with cell area decrease and filamentous actin (F-actin) breakdown, processes that can influence the impedance. Lack of F-actin breakdown and an increase in cell area during melatonin-induced aggregation suggest that some other intracellular process also contributes to the impedance decrease seen for melatonin. It was shown that impedance measurements reflect not only cell attachment and cell spreading, but also intracellular events.     

退变性腰椎滑脱CT下小关节面角的测量与意义

目的 经ＣＴ观测退变性腰椎滑脱病人及正常人的腰椎小关节面角,推测矢状方向小关节与退变性腰椎滑脱之间的关系。方法 包括２４例Ｌ４～５节段的退变性滑脱病人和３７例正常人作为研究对象,均为女性。应用ＣＴ对Ｌ３～Ｓ１腰椎小关节进行扫描和小关节面角测量。结果 除观察到明显的退行性变化外,滑脱病人的Ｌ４～５节段小并节面角明显小正常人群。结论 过度朝向矢状面的退变小关节是导致腰椎退变性滑脱的重要原因之一。