全文获取类型
收费全文 | 2714篇 |
免费 | 235篇 |
国内免费 | 31篇 |
专业分类
耳鼻咽喉 | 10篇 |
儿科学 | 88篇 |
妇产科学 | 28篇 |
基础医学 | 485篇 |
口腔科学 | 82篇 |
临床医学 | 283篇 |
内科学 | 753篇 |
皮肤病学 | 61篇 |
神经病学 | 168篇 |
特种医学 | 301篇 |
外科学 | 247篇 |
综合类 | 25篇 |
预防医学 | 142篇 |
眼科学 | 21篇 |
药学 | 124篇 |
中国医学 | 1篇 |
肿瘤学 | 161篇 |
出版年
2022年 | 18篇 |
2021年 | 54篇 |
2020年 | 23篇 |
2019年 | 35篇 |
2018年 | 62篇 |
2017年 | 48篇 |
2016年 | 65篇 |
2015年 | 74篇 |
2014年 | 83篇 |
2013年 | 74篇 |
2012年 | 126篇 |
2011年 | 107篇 |
2010年 | 77篇 |
2009年 | 69篇 |
2008年 | 56篇 |
2007年 | 82篇 |
2006年 | 73篇 |
2005年 | 81篇 |
2004年 | 69篇 |
2003年 | 81篇 |
2002年 | 76篇 |
2001年 | 74篇 |
2000年 | 56篇 |
1999年 | 61篇 |
1998年 | 78篇 |
1997年 | 91篇 |
1996年 | 85篇 |
1995年 | 65篇 |
1994年 | 52篇 |
1993年 | 56篇 |
1992年 | 47篇 |
1991年 | 40篇 |
1990年 | 70篇 |
1989年 | 81篇 |
1988年 | 72篇 |
1987年 | 57篇 |
1986年 | 58篇 |
1985年 | 61篇 |
1984年 | 23篇 |
1983年 | 29篇 |
1982年 | 17篇 |
1981年 | 22篇 |
1980年 | 23篇 |
1979年 | 29篇 |
1978年 | 21篇 |
1977年 | 23篇 |
1976年 | 22篇 |
1975年 | 20篇 |
1972年 | 18篇 |
1968年 | 23篇 |
排序方式: 共有2980条查询结果,搜索用时 15 毫秒
1.
Isolated patellofemoral arthritis is an increasingly recognized entity, and is usually associated with previous patellofemoral dysplasia or instability. Patellofemoral arthroplasty (PFA) has evolved significantly in recent years, both in terms of implant design and importantly in the understanding of appropriate patient selection. This review outlines the indications and investigations for PFA, provides a brief history of the development of contemporary implants, and presents the clinical outcomes for the prostheses most commonly used in the UK. In addition, it provides a detailed surgical technique for implantation of an onlay implant, with tips on how to optimize patellofemoral biomechanics and thus achieve a consistently good outcome. 相似文献
2.
3.
B L Thiele 《The Surgical clinics of North America》1990,70(1):1-11
The diversity of testing methodologies and reporting practices has necessitated that standards be defined for both performance of testing as well as interpretation and reporting of results. The guidelines to be used for the noninvasive diagnosis of extracranial vascular disease and the rationale for their use are presented. Emphasis is placed upon realistic diagnostic features that have clinical relevance and the use of testing methods that can identity these features. Further developments in this field, particularly those addressing the overall performance of vascular diagnostic laboratories, are to be defined by a multiprofessional inter-societal commission in the near future. It is anticipated that a voluntary accreditation process will insure high quality of testing. 相似文献
4.
Sonoelasticity imaging of prostate cancer: in vitro results 总被引:2,自引:0,他引:2
5.
6.
Geoffrey Thiele Molly Bicak Helen Grierson Patrick Lai David Purtilo 《Journal of immunological methods》1987,100(1-2):249-259
An enzyme-linked immunosorbent assay (ELISA) was used to measured IgG antiboody titers againt a synthetic peptide whose sequence was derived from the glycine-alanine repeating region of Epstein-Barr virus nuclear associated antigen 1 (EBNA-1). Antibody titers were determined in sera from 15 normal subjects, sera from 21 normal male siblings of X-linked lymphoproliferative syndrome (XLP) patients, from 20 XLP patients comprising a total of 42 samples, and ten samples before and ten samples after gamma-globulin therapy in ten patients with XLP. Data analysis demonstrated that while there are differences between the ELISA and ACIF, they appear to measure a similar response as demonstrated by their correlation coefficient (0.77) and the GMT to EBNA observed by both methods. No cross-reactivity of cytomegalovirus antibodies to the EBNA-1 peptide was observed by immunobv using adsorption against AD-169 infected MRC-5 cells.. However, non-specific binding was observed if samples were not pre-incubated in a 10% goat serum PBS-Tween 20 solution. This pre-treatment removed the non-specific binding that falsely elevated GMT in approximately 15% of both normal and XLP samples in ELISA. The ELISA system appears to be a sensitive, reproducible and objective test that may be useful for assessing the antibody responses of patients to the EBNA-1 protein. 相似文献
7.
8.
J. Thiele T. K. Zirbes J. Lorenzen H. M. Kvasnicka S. Scholz A. Erdmann U. Flucke V. Diehl R. Fischer 《Annals of hematology》1997,75(1-2):33-39
In order to determine the dynamics of hematopoietic cell turnover, proliferative activity and incidence of apoptosis (programmed
cell death) were evaluated in bone marrow trephine biopsies. Selection of patients (20 in each group) included in addition
to a control group, idiopathic thrombocytopenia (ITP), reactive thrombocytosis (TH), secondary polycythemia-smokers' polyglobuly
(PG), primary (essential-hemorrhagic) thrombocythemia (PTH), polycythemia vera (PV), and finally acute myeloid leukemia (AML).
Apoptosis was demonstrated by the in situ end-labeling technique (ISEL) and proliferative activity by applying the monoclonal
antibody PC10 raised against proliferating cell nuclear antigen (PCNA). To assess dynamic features of hematopoiesis, an index
was calculated consisting of the ratio between PCNA-positive nuclei and the apoptotic cell fraction. This factor was termed
the hematopoietic turnover index (HTI). Morphometric analysis revealed that the HTI was significantly increased in AML and
PV. According to cell culture studies both disorders are characterized by either a prevalent proliferation of the myeloid
or erythroid cell mass. On the other hand, PG, PTH, and TH showed no relevant enhancement of this index in comparison to the
control specimen. In vitro experiment results are in keeping with the finding that PG and PTH are not associated with a significant
expansion of the erythroid lineage (CFU-E). Similar to ITP and TH, in PTH megakaryocyte proliferation (CFU-MEG) is the predominant
feature of cell turnover. Differences between PTH and TH are in line with the reduced in vitro formation of CFU-MEG in the
latter disorder. In conclusion, our in situ study on turnover rates of the bone marrow in various neoplastic and reactive
lesions extends previous experimental data on hematopoietic cell kinetics.
Received: 10 March 1997 / Accepted: 18 May 1997 相似文献
9.
10.
L H Anderson T L McDonald G M Thiele L W Klassen 《Journal of clinical laboratory analysis》1992,6(3):113-118
Different assay systems have been used to quantitate lymphokine-induced natural cytotoxic activity as a measure of immune status. This study compares the effects of inducing cytotoxicity in a bulk culture system, where effector cells are transferred to a micro culture well for assay, to a micro culture system where the effector cells are not transferred. The effector/target ratio for both the bulk and micro culture systems was calculated using the number of viable effector cells present at the time of target cell addition. After overnight incubation with interleukin-2 (IL-2), the lytic activity of murine spleen cells to targets using a micro culture system was increased two-fold over the bulk culture method. This increase was amplified further after 5 days of activation with IL-2, in that the micro culture system resulted in a four-fold increase in cytotoxic activity. The loss of some adherent cells in the bulk culture system did not explain the overall decrease in recovered cytotoxicity. The difference appeared to be related to cell loss during centrifugation. Therefore, the E/T ratios are different in the two systems if not corrected for the number of viable cells. 相似文献