Wound healing effect of flavonoid rich fraction and luteolin isolated from Martynia annua Linn. on streptozotocin induced diabetic rats

ObjectiveTo evaluate wound healing potential of flavonoid fractions of Martynia annua (M. annua) Linn. leaves in diabetic rats on the basis of folkloric information and preliminary study.MethodsThe flavonoid compound luteolin and apigenin were isolated from dried leaves of plant by column chromatography. The two concentrations (0.2% and 0.5% w/w) of luteolin and flavonoid fraction were selected for topically applied as ointment on diabetic wound. The Povidone Iodine Ointment USP was used as a reference. On 18th days, protein content, hydroxyproline and antioxidants (SOD, CAT and GSH) level in granuloma tissues were determined.ResultsThe results showed that, percent wound contraction were observed significantly (P<0.01) greater in MAF fraction and 0.5% w/w of luteolin treatment groups. Presence of matured collagen fibres and fibroblasts with better angiogenesis were observed in histopathological studies.ConclusionsIn conclusion, our findings suggest that flavonoid fraction (MAF) and luteolin (0.5% w/w) may have potential benefit in enhancing wound healing in diabetic condition, possibly due to free-radical scavenging activity of plant.     

Preliminary pharmacological evaluation of Martynia annua Linn leaves for wound healing

Objective

To evaluate the wound healing potential of fractions from ethanol extract of Martynia annua (M. annua) Linn leaves.

Methods

Ethanol extract of M. annua Linn leaves was fractionate into three different fractions (MAF-A, MAF-B and MAF-C) which were screened for wound healing potential using two models: excision and incision on rats. The thin layer chromatography (TLC) profile of all fractions were analyzed and TLC of luteolin was also done. The Povidone-Iodine Ointment was used as reference for comparision. Excision and incision wounds were created on dorsal portion of rats for study. Wound contraction, biochemical parameters (protein level and hydroxyproline level) and histopathological study were performed in excision wound model whereas incision model was used for determination of tensile strength.

Results

The wound contraction and tensile strength of skin tissues were observed significantly greater in MAF-C fraction treated group than other two fractions (P<0.01). In excision wound method (on day 18) protein content and hydroxyproline were found significantly higher in MAF-C group than control group (P<0.01). Histopathological study also showed better angiogenesis, matured collagen fibres and fibroblast cells as compared with the control group.

Conclusions

In conclusion, our findings suggest that fraction MAF-C from ethanol extract of M. annua leaves is found most effective in wound healing.     

Ameliorative Effect of Polysaccharide Rich Fraction from Eulophia herbacea Against Methotrexate Induced Liver Damage in Rats

Pharmaceutical Chemistry Journal - The present study was aimed to investigate a protective effect of polysaccharide rich fraction from Eulophia herbacea Lindl. tubers against methotrexate (MTX)...     

Structure – Activity Relationship and Therapeutic Benefits of Flavonoids in the Management of Diabetes and Associated Disorders

Pharmaceutical Chemistry Journal - Commonly flavonoids are the natural components found in vegetables, spices and colored fruits. Flavonoids include a large group of polyphenolic compounds, namely...     

西印度醋栗对四氯化碳引起的大鼠和小鼠急性过氧化肝损伤的保护作用

目的：证实西印度醋栗（Phyllanthus acidus（L.）Skeels）的果实在传统医学中作为保肝药使用的药用用途。方法：连续5d分别给予各组大鼠西印度醋栗果实的70%乙醇提取物（100,200和400mg/kg口服）及对照药水飞蓟素（100mg/kg口服）并于第2天及第3天皮下注射四氯化碳（2mL/kg）。测定血清天冬氨酸氨基转移酶（aspartate transaminase,AST）、丙氨酸氨基转移酶（alanine transaminase,ALT）、碱性磷酸酶（alkaline phosphatase,ALP）、总胆红素以及总蛋白并进行肝脏组织病理学检测。检测大鼠肝组织匀浆中的氧化应激标记物如脂质过氧化反应（lipid peroxidation,LPO）、还原型谷胱甘肽（reduced glutathione,GSH）、超氧化物歧化酶（superoxide dismutase,SOD）、过氧化氢酶（catalase,CAT）以及谷胱甘肽过氧化物酶（glutathione peroxidase,GPx）以衡量西印度醋栗的体外抗氧化作用。此外,本实验还记录了伊维巴诱导的给药后小鼠睡眠时间,使用2,2-二苯基-1-苦基肼（2,2-diphenyl-1-picrylhydrazil,DPPH）法测定小鼠服药后的自由基清除率。结果：与对照组相比,西印度醋栗果实的乙醇提取物及水飞蓟素均显著降低了模型大鼠血清AST、ALT和ALP水平及LPO并升高了TP、GSH、SOD、CAT和GPx的水平（P〈0.01或P〈0.05）。大鼠肝脏组织的组织病理学切片结果也证实了以上实验结果。在小鼠实验中,与对照组相比,西印度醋栗果实的乙醇提取物显著缩短了伊维巴诱导的小鼠睡眠时间（P〈0.01）,并具有较高的DPPH清除率。结论：本研究的实验结果证实了西印度醋栗果实对四氯化碳引起的大鼠急性过氧化肝损伤的保护作用,机制可能与其抗氧化作用及自由基清除功能有关     

Erythropoietic activity of Asteracantha longifolia (Nees.) in rats

Ethnopharmacological relevance

Asteracantha longifolia Nees. (Family-Acanthaceae) is a wild herb commonly used in traditional ayurvedic medicine as Kokilaaksha and the Unani drug as Talimakhana in India and Srilanka for various medicinal uses as aphrodisiac, tonic, sedative and blood diseases etc.

Aim of the study

The aim of the current study was to validate and explore the folk use of Asteracantha longifolia Nees. (AL) (Leaf part) on pharmacological grounds using haloperidol induced iron deficiency anemia for the assessment of erythropoietic activity.

Materials and methods

Determination of iron in plant extracts was carried out using spectrophotometric method. Plant extract was obtained from crude drugs using extraction with ethanol. In vivo study, haloperidol induced iron deficiency anemia model was used in experimental studies.

Results

An administration of ethanolic extract of AL at the doses of 100 mg/kg and 200 mg/kg body weight, i.p., demonstrated a significant (P < 0.05) increase in erythrocyte count, haemoglobin count, serum iron and serum protein etc. This effect may be due to the presence of iron (622 μg/50 mg) in extract estimated by spectrophotometric method.

Conclusion

An ethanolic extract of AL effectively restored the hematological parameters, serum iron and serum protein and normalized the microcytic (smaller in size), anisocytosis (disturbed shape) and hypochromic RBCs. These observations could justify the inclusion of this plant in the management of iron deficiency anemia due the presence of iron and other constituents as flavonoids, terpenoids, steroids, lupeol and betulin.     

Wound healing potential of Tephrosia purpurea (Linn.) Pers. in rats

Tephrosia purpurea is a well-known herb for its hepatoprotective, anticancer, antiulcer, antibacterial and in healing bleeding piles, etc. The present study was aimed for wound healing potential of ethanolic extract of Tephrosia purpurea (aerial part) in the form of simple ointment using three types of wound models in rats as incision wound, excision wound and dead space wound. The results were comparable to standard drug Fluticasone propionate ointment, in terms of wound contraction, tensile strength, histopathological and biochemical parameters such as hydroxyproline content, protein level, etc. Histopathological study showed significant (P<0.05) increase in fibroblast cells, collagen fibres and blood vessels formation. All parameters were observed significant (P<0.05) in comparison to control group.