Functional state of the esophagus in cardiospasm according to esophagomanometry data

Functional changes occurring in the esophagus at different stages of cardiospasm revealed by esophagomanometry are presented. Esophagomanometry is helpful for making a more precise diagnosis, for identifying the stage of the disease, and determining treatment. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 118, N ^o 9, pp. 334–336, September, 1994 Presented by T. T. Berezov, Member of the Russian Academy of Medical Sciences     

The membranotropic activity of cyclic acyldepsipeptides from bacterium Bacillus pumilus, associated with the marine sponge Ircinia sp.

The isolate of Bacillus pumilus associated with the marine sponge Ircinia sp. produced the surfactin-like lipopeptides, cyclic acyldepsipeptides. The hemolytic activity of individual cyclic acyldepsipeptides, bacircines (BI) 2, 3, 4, 5 and 5A having different acyl side chain structures (anteiso-C13, iso-C14, normal-C14, anteiso-C15, and iso-C15, respectively) was studied. The hemolytic power of bacircines depended on both the structure of the side chain (n->iso->anteiso-) and pH values (5.6 and 6.5 > 7.4). Hemolytic potency as a function of BI 5 concentration was given for pH 6.5; 7.4; 8.0; 9.0. pH dependent hemolysis induced by BI 5 was shown to be reversible. The membrane damaging potential of bacircine 5 (5 microM) at pH 6.5 was characterized by a higher rate of hemolysis and by a shorter time between the introduction of BI 5 solution into the RBC samples and the onset of hemolysis. Under this condition, BI 5 decreased abnormally the microviscosity of erythrocyte ghosts bilayer. The damaging potency of BI 5 decreased with an increase pH from 6.5 to 7.4 or its decrease from 6.5 to 4.9. It was shown that fatty acid bacircine fragment penetrated into the lipid bilayer to a depth of minimum 7 carbon atoms. Constants of dissociation of the Asp (pK 4.75) and Glu (pK 6.65) residues of bacircine in the lipid bilayer were obtained. These results showed that at pH 6.5 BI 5 possessed membranotropic activity in the monoionic form.     

New diterpenic altrosides of the fungus Acremonium striatisporum isolated from a sea cucumber

Two new diterpenic glycosides, virescenosides M (1) and N (2), have been isolated from a marine strain of Acremonium striatisporum KMM 4401 associated with the holothurian Eupentacta fraudatrix. Their structures were determined on the basis of MS and NMR data as beta-D-altropyranosido-19-7-oxo-isopimara-8,15-diene-2alp ha, 3beta-diol (1) and beta-D-altropyranosido-19-isopimara-7, 15-diene-2alpha,3beta,6beta-triol (2). Three other altrosides (3-5), identified as virescenosides A, B, and C from the terrestrial strain Acremonium luzulae, were also isolated. The cytotoxic activity of the virescenosides was examined.