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排序方式: 共有408条查询结果,搜索用时 15 毫秒
1.
Summary. Bovine rotavirus strain 678 is the first G8 strain of bovine origin but the literature is confusing as to its P type. In
this study, two-way cross neutralization between 678 and 0510, a prototype G6P7[5] virus, was shown by plaque-reduction neutralization
assays, establishing the P type of 678 as being P7[5]. The P7[5] specificity of 678 VP4 was reinforced by the finding that
the VP8* portion of 678 VP4 had the highest amino acid identity with those of P7[5] bovine rotaviruses. Apparent contradiction with
previous serological studies relates to intricacy of antigenicity and immunogenicity of UK VP4 in reassortants.
Received October 28, 1996 Accepted January 6, 1997 相似文献
2.
K Nakagomi S Sakai H Tanaka N Tomizuka Y Kawakami T Nakajima 《The Journal of antibiotics》1990,43(5):470-476
The planar structures of new eurocidin related compounds, eurocidins D and E, were elucidated from 1H-1H shift correlated 2D NMR spectra and other NMR data. All protons in the molecules were assigned. Eurocidins D and E have novel pentaenic structures of eurocidin family. 相似文献
3.
H Oyamada O Nakagomi S Uesugi 《Rinsho byori. The Japanese journal of clinical pathology》1992,40(1):9-15
C-reactive protein (CRP) is a single most important test among various serological tests which are routinely performed in clinical laboratories. While qualitative assays for CRP are being replaced by quantitative assays, standardization among various laboratories has become an urgent issue. The results of the past three-year surveys conducted by the Japanese Medical Association showed that the coefficients of variation (CV) for CRP assays ranged between 12.6% and 46.9% (1990), although these figures gradually changed for the better. These results clearly indicate that a CRP value obtained in one laboratory cannot be compared directory with that obtained in another laboratory. Every assay for CRP is considered to show good reproducibility, however, which is predicted by the results of within-run precision tests (CV:3.9-7.8%). It is very important to establish standardization for CRP assays but one has to conclude that the path toward this goal is very difficult when one takes into consideration various factors inherent to immunological reactions of macromolecules including possible microheterogeneity of CRP molecules. 相似文献
4.
Genetic analysis of a human rotavirus that belongs to subgroup I but has an RNA pattern typical of subgroup II human rotaviruses. 总被引:6,自引:13,他引:6
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O Nakagomi T Nakagomi Y Hoshino J Flores A Z Kapikian 《Journal of clinical microbiology》1987,25(7):1159-1164
We have previously found (O. Nakagomi, T. Nakagomi, H. Oyamada, and T. Suto, J. Med. Virol. 17:29-34, 1985), during an epidemiological study in Japan, a novel human rotavirus that belongs to subgroup I but has a long RNA pattern typical of subgroup II human rotaviruses. From the stool specimen containing this virus, we successfully isolated in MA104 cells a rotavirus, designated AU-1, which possesses these novel characteristics. The possibility that strain AU-1 was a laboratory contaminant of an animal rotavirus previously adapted to tissue culture cells was ruled out, and the identity of the AU-1 strain was established. Genetic analysis by RNA-RNA hybridization revealed that the AU-1 strain is not a simple reassortant between subgroup I and II human rotaviruses but that it shares a high level of sequence homology only with the gene encoding VP7 (the major neutralization protein) of serotype 3 human rotaviruses. Weak homology of the genomic RNA segments was also observed between the AU-1 strain and animal rotavirus strains, including rhesus rotavirus strain RRV and bovine rotavirus strain NCDV. These results suggest that the AU-1 strain may be an animal rotavirus that infected a human. 相似文献
5.
Relative frequency of VP4 gene alleles among human rotaviruses recovered over a 10-year period (1982-1991) from Japanese children with diarrhea. 总被引:1,自引:5,他引:1
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S Gunasena O Nakagomi Y Isegawa E Kaga T Nakagomi A D Steele J Flores S Ueda 《Journal of clinical microbiology》1993,31(8):2195-2197
The relative frequencies of the Wa (corresponding to serotype P1A), DS-1(P1B), M37(P2), and AU-1(P3) alleles of the VP4 gene from rotaviruses collected from the stools of individuals in Japan between 1982 and 1991 were determined to be 83.1, 15.6, 0, and 1.3%, respectively, by a polymerase chain reaction-based typing assay. 相似文献
6.
Relative frequencies of G (VP7) and P (VP4) serotypes determined by polymerase chain reaction assays among Japanese bovine rotaviruses isolated in cell culture. 总被引:3,自引:5,他引:3
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Y Suzuki T Sanekata M Sato K Tajima Y Matsuda O Nakagomi 《Journal of clinical microbiology》1993,31(11):3046-3049
The relative frequencies of both the G (VP7) and P (VP4) serotypes of 40 bovine rotaviruses isolated in cell culture from diarrheic calves in Japan between January 1983 and February 1991 were determined by recently developed polymerase chain reaction assays. Isolates with G serotype 6 and P serotype 5 (UK-like strains) were most frequently found (42.5%) followed by isolates with G6P11 (17.5%), G6P1 (10%), or G10P5 (10%). Isolates with G10P11 (B223-like strains) were least frequently found (7.5%). The presence of various combinations of G and P serotypes suggests frequent reassortment in nature among bovine rotaviruses. 相似文献
7.
Adachi H Kume A Li M Nakagomi Y Niwa H Do J Sang C Kobayashi Y Doyu M Sobue G 《Human molecular genetics》2001,10(10):1039-1048
We generated transgenic mice that expressed a highly expanded 239 polyglutamine (polyQ) repeat under the control of the human androgen receptor promoter. These transgenic mice developed progressive neurological phenotypes of muscular weakness and ataxia, small body size and short life-span. PolyQ nuclear inclusions (NIs) were remarkable and widespread but found in selective regions of the central nervous system (CNS) such as the spinal cord, cerebrum and cerebellum as well as in selective peripheral visceral organs. This distribution pattern resembled that of spinal and bulbar muscular atrophy somewhat, but was more widespread. In neuronal tissues, NIs were present in astrocytes as well as neurons. Cytoplasmic and axonal inclusions were not observed. In the CNS regions with abundant NIs, neuronal populations were well-preserved, and neither neuronal cell death, reactive astrogliosis nor microglial invasions were detected. These findings suggest that polyQ alone can induce the neuronal dysfunction that precedes gross neuronal degeneration and provides a clue for investigating molecular mechanisms that underly the pathway to neuronal dysfunction from polyQ expansion. 相似文献
8.
Molecular identification of a novel human rotavirus in relation to subgroup and electropherotype of genomic RNA 总被引:3,自引:0,他引:3
O Nakagomi H Oyamada S Kuroki Y Kobayashi A Ohshima T Nakagomi 《Journal of medical virology》1989,28(3):163-168
A total of 41 stool rotavirus specimens collected from children with acute diarrhea at four different locations in Akita Prefecture, Japan, during the peak of the winter diarrhea epidemic in 1988 were analyzed by polyacrylamide gel electrophoresis of viral RNA in conjunction with subgrouping assay. We found that a single strain predominated, with cocirculating strains with less common electropherotypes at a given location, and that two different strains could predominate at geographically close but different locations even during a very limited time of the epidemic season. Furthermore, we isolated a human rotavirus strain (AU125) that was similar to the AU-1 strain in that it possessed a long RNA pattern yet belonged to subgroup I. Genetic analysis by RNA-RNA hybridization assay indicated that the AU125 strain was distinct from two previously identified human rotavirus gene groups (genogroups) represented by the Wa strain (subgroup II with long RNA electropherotype) and the DS-1 strain (subgroup I with short RNA electropherotype), but was very closely related to the AU-1 strain. These data suggest that the genetic diversity of human rotaviruses may be more extensive than was previously thought. 相似文献
9.
10.
Serotypic and genotypic characterization of human serotype 10 rotaviruses from asymptomatic neonates. 总被引:2,自引:7,他引:2
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S J Dunn H B Greenberg R L Ward O Nakagomi J W Burns P T Vo K A Pax M Das K Gowda C D Rao 《Journal of clinical microbiology》1993,31(1):165-169
Human rotaviruses were isolated from asymptomatic neonates at various hospitals and clinics in the city of Bangalore, India, and were found to be subgroup I specific and possess long RNA patterns (M. Sukumaran, K. Gowda, P. P. Maiya, T. P. Srinivas, M. S. Kumar, S. Aijaz, R. R. Reddy, L. Padilla, H. B. Greenberg, and C. D. Rao, Arch. Virol. 126:239-251, 1992). Three of these strains were adapted to tissue culture and found by serotype analysis and neutralization assays to be of serotype 10, a serotype commonly found in cattle but infrequently found in humans and not previously identified in neonates. By RNA-RNA hybridization, a high level of relatedness to a serotype 10 bovine rotavirus strain and a low-to-medium level of relatedness to a human rotavirus strain were observed. Since this human isolate shares a genogroup with bovine rotavirus, it is likely that it originated by interspecies transmission. A human rotavirus strain isolated from asymptomatic neonates and similar to bovine rotavirus might represent a good vaccine candidate. 相似文献