8周基础军训对入伍新兵红细胞及其相关指标的影响

目的探讨8周基础军训(basic military training,BMT)对入伍新兵血像中红细胞及其相关指标的影响,为指导科学的军事训练提供参考。方法数据来自新疆边防部队2015年度入伍的50名男性新兵,分别在BMT前后测定并记录受试新兵的红细胞计数、血红蛋白浓度及血清铁蛋白等。结果经过8周的BMT,新兵血液中血红蛋白浓度、红细胞计数及血清铁蛋白均显著下降(P0.05,P0.01)。结论 8周BMT可能导致入伍新兵发生运动性贫血,铁缺乏可能是其主要原因。     

生理盐水肠道冲洗辅助治疗真菌性肠炎效果观察

目的 探讨生理盐水肠道冲洗辅助治疗真菌性肠炎的疗效。方法 将72例真菌性肠炎患者随机分为观察组和对照组各36例，对照组行常规治疗，观察组在此基础上予以温生理盐水（38～41℃）500～7000ml持续肠道冲洗，3～6h／次，1次／d，治疗1～3次。结果 观察组治疗后肠道pH值显著低于治疗前及对照组治疗后（均P〈0．01），治愈率显著高于对照组（P〈0．01）。结论 对真菌性肠炎腹泻患者在常规治疗的基础上，辅以温生理盐水肠道冲洗可显著改善肠道环境，提高治愈率。     

22例皮质醇增多症的临床研究

目的：探讨皮质醇增多症的临床表现和内分泌检查等辅助检查的意义.方法：从年龄、性别、病程及实验室检查等方面,观察22例不同原因所致皮质醇增多症患者不同的临床表现和测定实验室检查指标.结果：22例中诊断库欣病（增生型）14例[63.6％,其中13例（92.9％）得到MRI检查证实],肾上腺腺瘤6例[27.3％,均得到MRI检查证实（100％）],另有肾上腺结节样增生1例（4.5％）,异位ACTH综合征1例（4.5％）.临床表现：按出现的频率前4位依次为,库欣病：高血压（100％）、满月脸（92.9％）、向心性肥胖（85.7％）、多血质（85.7％）,肾上腺腺瘤：高血压（100％）、满月脸（100％）、向心性肥胖（100％）、多血质（83.3％）.实验室检查：小剂量地塞米松不能抑制：库欣病与肾上腺腺瘤均为100％.结论：根据高血压、满月脸、向心性肥胖,小剂量地塞米松抑制试验和MRI检查可诊断绝大多数皮质醇增多症.     

苦参素注射液治疗慢性丙型肝炎肝硬化合并糖尿病

【目的】探讨苦参素注射液治疗慢性丙型肝炎肝硬化合并糖尿病的效果。【方法】将86例慢性丙型肝炎肝硬化合并糖尿病患者随机分为静滴苦参素治疗组（A组）和基础保肝治疗对照组（B组），疗程均为3个月。【结果】治疗前后肝功能复常率、血糖好转率、HCV-RNA转阴率有明显差别（P〈0．05）。肝纤维化指标透明质酸酶（HA）、Ⅲ型前胶原（PCⅢ）治疗后有明显下降（P〈0．01），且苦参素治疗组未见明显不良反应。【结论】苦参素注射液对促进肝功能恢复、血糖好转和抑制丙肝病毒复制有一定效果，具有明显的抗纤维化的作用。     

改良性治疗法联合西地那非治疗勃起功能障碍维吾尔族患者2505例回顾性分析

目的:性治疗法目前尚未普及,本研究旨在评价性治疗法联合西地那非治疗勃起功能障碍(ED)的疗效。方法:根据治疗方法的不同将在本院治疗随访过的3130例维吾尔族ED患者分成2组。对照组625例,单纯口服西地那非3个月;试验组2505例,采用性治疗法联合西地那非治疗3个月。采用国际勃起功能问卷表(IIEF-5)在各组治疗前、后进行疗效评估,并随访12个月。结果:对照组治疗前、后及在6个月、12个月随访的IIEF-5评分分别为12.80±3.76、18.10±2.61、17.35±2.73和16.64±2.63;试验组治疗前、后及在6个月、12个月随访的IIEF-5评分分别为12.73±3.52、19.06±4.07、19.86±2.42和20.47±2.38。两组治疗前后IIEF-5评分自身对比差异均有显著性(P<0.05)。组间比较,试验组较对照组6个月和12个月随访IIEF-5评分均有显著性差异(P<0.05)。结论:性治疗法联合西地那非治疗ED的效果优于单纯西地那非治疗,并在12个月的随访中稳定性良好。     

The biography of Mary E. O'Sullivan: an early American headache specialist

The aim of this study was to review the life of Mary E. O'Sullivan and to summarize her important contributions to the study of migraine. Mary E. O'Sullivan underwent extensive training to become a neurologist at a time when only 5% of women in America were physicians. She published five papers on migraine. In a 1936 Journal of the American Medical Association article, she described a patient with ergotamine overuse headache and recommended that daily doses of oral ergotamine should be avoided. Three years later she described migraine as a 'complex' syndrome with multiple causes and multiple cures. Mary E. O'Sullivan, an ambitious female headache specialist of the 1930s, was an early advocate of the use of ergotamine to treat migraine, yet she was one of the first to report ergotamine overuse headache. Although her life was short, her research, knowledge and ambition at a time when women had limited opportunities in medicine have left a mark.     

一类新的羟基苯二磺酰苯胺类化合物的合成及其杀虫活性

目的合成并筛选出杀虫活性较好的新型抗肝片吸虫病化合物。方法分别以邻(间、对)氯苯酚为原料，通过磺酰化和亲核取代反应合成了14种羟基苯二磺酰苯胺类化合物;通过元素分析、红外光谱及核磁共振氢谱对合成的14种新型抗肝片吸虫病化合物羟基苯二磺酰苯胺类化合物(1～14)的结构进行了表征,并测定其对线粒体呼吸控制率的影响以及线粒体呼吸过程无机磷的变化,以此对化合物的解偶联活性(杀虫活性)进行了评价。结果所合成的大部分羟基苯二磺酰苯胺类化合物均具有较好的解偶联活性,其中3,5,6,9的杀虫活性最好。结论 化合物3,5,6,9均有望成为较好的新型抗肝片吸虫病药物。     

三仙汤对成骨细胞增殖的影响

目的通过对三仙汤促进成骨细胞株增殖的研究,判断三仙汤能否应用于绝经后骨质疏松症的防治。方法应用UMR-106成骨细胞株,采用体外细胞培养技术,以氟化钠为阳性对照药,用MTT法测定三仙汤对成骨细胞增殖的影响。结果氟化钠1.25μmol/L、5μmol/L组与空白组相比有显著性差异(前者P<0.01,后者P<0.001);10μmol/L组与空白组无明显差别。三仙液在5、10、100、500、1000mg/L5个不同浓度均使MTT法检测的光吸收值显著升高(P<0.001或P<0.01)。结论三仙汤能促进体外培养成骨细胞增殖。     

Gamma-carboxylated isoforms of recombinant human protein S with different biologic properties

Human protein S (HPS), a regulator of hemostasis, is a vitamin K- dependent plasma protein with potential clinical utility. We have obtained high-level expression of the cDNA for HPS in two mammalian cell lines. Both cell lines secreted single chain recombinant HPS (rHPS) in serum-free medium as determined by Western blot analysis. The ability of the rHPS from both cell lines to act as a cofactor for human protein C (HPC) was determined; the rHPS secreted from the human 293 cell line had an activity six times that of the rHPS from the AV12-664 Syrian hamster cell line. Furthermore, the relative specific cofactor activity of rHPS from the 293 cell line was actually 2.5-fold higher than that of single-chain human plasma-derived HPS. Essentially all of the rHPS secreted from the 293 cell line exhibited a calcium-dependent elution profile on anion exchange chromatography, whereas only 25% to 35% of the hamster cell-derived rHPS exhibited this profile. However, the calcium-eluted rHPS from the AV12 cell line had a high specific cofactor activity, equivalent to that of the 293-derived rHPS. A NaCl- elutable rHPS fraction (calcium nondependent) was isolated from the recombinant AV12-664 cell line, further purified, and found to have reduced activity, only 40% that of the calcium-dependent rHPS. The only observable difference in the calcium-dependent and nondependent rHPS molecules was in the content of gamma-carboxyglutamic acid (Gla); the calcium-dependent material contained approximately 10 mol Gla/mol protein whereas the calcium-nondependent material contained only approximately 8 mol Gla/mol of protein. In addition, the calcium- nondependent rHPS had reduced ability to interact with phospholipid vesicles as evidenced by an eightfold increase in the apparent kd. Our data demonstrate the isolation of rHPS with high specific activity, and show that a reduction in as few as two Gla residues dramatically decreases its functional cofactor activity for HPC, due to a reduction in ability to interact with the phospholipid bilayer.     

坚固内固定技术在颌骨骨折治疗中的临床应用分析

[目的]探讨坚固内固定技术在颌骨骨折治疗中的临床应用价值。[方法]对某院2009年9月～2011年2月收治的颌骨骨折患者采用坚固内固定加颌间牵引治疗,并与单独采用坚固内固定治疗的对照组进行了临床疗效的比较。[结果]小型钛板坚固内固定加颌间牵引治疗的观察组的治疗优良率明显优于仅采用小型钛板坚固内固定治疗的对照组,且差异有统计学意义(χ2=5.00,P﹤0.05)。[结论]小型钛板坚固内固定在治疗下颌骨骨折中的应用改变了传统的颌骨骨折治疗方法,该方法在颌骨骨折治疗中与传统的颌间固定和骨间固定方法相比,具有效果可靠、操作简便、创伤小、对位精确、固定稳固和允许下颌即时或早期进行生理活动等优点。术后加颌间牵引对颌骨骨折具有良好的骨折愈合和功能恢复作用。