Effect of Histamine Receptor Blocking on Human Antibody-dependent Cell-mediated Cytotoxicity

The effect of H1 and H2 receptor-blocking agents on antibody-dependent cell-mediated cytotoxicity (ADCC) was studied. The H1 receptor-blocker clemastinum and the H2 receptor blocker cimetidine dose-dependently inhibited the antibody-dependent cytotoxic activity of normal human peripheral blood mononuclear cells on chicken erythrocytes. The inhibition cannot be explained either by a direct toxic effect on effector cells or by blocking of Fc receptors. The possible involvement of histamine receptor-bearing effector cells in human ADCC is suggested.     

Reactivity of Hybridoma Antibodies with Amniotic and Plasma Fibronectin

Mouse antibodies were prepared to human amniotic fluid fibronectin by means of somatic cell hybrids. Comparison of immunological reactivity of antibodies with amniotic and plasma fibronectin revealed that the two molecules had very similar patterns of antigenic determinants. Antibodies from one cell population, however, bound belier to amniotic fluid fibronectin, indicating that there is a difference in the molecular structure of fibronectins isolated from the two sources.     

Strand-Specific Reverse Transcription PCR for Detection of Replicating SARS-CoV-2

We developed an assay that detects minus-strand RNA as a surrogate for actively replicating severe acute respiratory syndrome coronavirus 2. We detected minus-strand RNA in 41 persons with coronavirus disease up to 30 days after symptom onset. This assay might inform clinical decision-making about patient infectiousness.     

Mycosis fungoides : Tumour D'emblee

A 60-years old male patient presented with nodules and noduloulcerative lesions of mycosis fungoides (MF) of 6 months duration without any previous premycotic, patch or plaque stage.     

泽泻提取物不同组分对尿草酸钙结石形成的实验研究

目的 :研究泽泻提取物不同组分对尿草酸钙结石形成的影响 ,并确定其抑制尿草酸钙结石形成的有效部位。方法 :通过喂服泽泻提取物不同组分对乙二醇和氯化铵诱导的大白鼠肾草酸钙结石模型进行实验研究。结果 :服用泽泻醋酸乙酯浸膏的醋酸乙酯洗脱液大鼠的血清尿素氮 (BUN) ,肌酐 (Cr) ,肾钙 (Ca²⁺ )含量 ,24h尿Ca²⁺ 分泌量 ,肾组织的草酸钙晶体沉积均明显低于成石组 (P<0.05)。结论 :泽泻醋酸乙酯浸膏的醋酸乙酯洗脱液能抑制实验性高草酸尿症大白鼠体内肾草酸钙晶体的形成 ,是泽泻抑制尿草酸钙结石形成的有效部位。     

喉鳞状细胞癌组织体外原代培养的初步研究

目的 应用体外培养技术,对喉鳞状细胞癌组织进行体外培养,探讨喉鳞癌组织原代培养中的各种影响因素,为建立人喉鳞癌组织的细胞系提供实验基础。方法采用体外组织培养技术,对24例人喉鳞状细胞癌组织进行原代培养,观察原代培养中肿瘤细胞的生长与供体的年龄、肿瘤组织的分化程度及不同培养方法的关系,分析在人喉鳞癌细胞的培养中成纤维细胞、微生物污染的影响。结果 24例人喉鳞状细胞癌组织标本,年龄小于60岁组的细胞生长率为31.25％(5/16例),年龄大于60岁组为37.5％(3/8例);高分化组为100％(2/2例),中分化组为30.8％(4/13例),低分化组25％(2/8例);组织块培养法为43.75％(7/16例),酶消化法培养为10％(1/10例);倒置显微镜下观察,在培养的第5～7天,在贴壁组织块周围可见到有上皮样细胞爬出。全部标本中,成纤维细胞的过度生长和微生物的污染是阻碍人喉鳞癌细胞生长的重要因素。结论培养组织的细胞生长率与供体的年龄关系不大;肿瘤组织的分化程度较高者,细胞的生长率较高;与酶消化分离培养法相比,贴壁组织块培养法的细胞生长率较高;成纤维细胞及微生物的污染是阻碍人喉鳞癌细胞系建立的重要因素。     

γ射线对大鼠血管平滑肌细胞增殖和周期影响

目的：探讨γ射线对大鼠血管平滑肌细胞（VSMCs）抑制作用机理。方法：对大鼠VSMCs进行原代培养，经^3H-TdR掺入法观察γ射线对VSMCs增殖情况影响，应用流式细胞仪检测γ射线对VSMCs细胞周期的影响。采用Western Blot技术检测γ射线对VSMCs p53、细胞周期素D（cyclin D)和增殖细胞核抗原（PCNA）表达的影响。结果：γ射线对寻殖的抑制作用呈剂量依赖性。γ射线照射可诱导VSMCs出现G1期阻滞，在照射后一定时间内会导致p53表达增强，cyclinD和PCNA表达减弱，结论：γ射线可抑制VSMCs增殖，其机制可能主要是γ射线诱导VSMCs出现G1期阻滞，抑制VSMCs进行有丝分裂，在这个过程中p53,cyclinD和PCNA等分子起到一定作用。