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Multiple antigenic peptides (MAPs) prepared for the predicted antigenic determinants on the VP2 protein of infectious bursal disease virus (IBDV) were used as antigens in enzyme-linked immunosorbent assay (ELISA)--an alternative to whole viral antigen to detect anti-IBDV antibodies in the chicken sera. Two MAPs were synthesized, which could specifically detect the anti-IBDV antibodies in serum samples by ELISA. The optimum quantity of MAP1 and MAP2 required to coat the wells of the ELISA plate was 5 ng/ml, whereas the amount of purified IBDV whole viral antigen was 500 ng/ml, indicating the high efficiency of MAPs. In this study, we mainly focused on the antigenicity of two eight-branched MAPs to detect anti-IBDV antibodies in ELISA, which would serve as safe, chemically defined, noninfectious alternative antigens to whole virus in serodiagnosis. The specificity and sensitivity of both MAP1 and MAP2 were found to be relatively better than the whole viral antigen. 相似文献
3.
Ramalingam?Saravanan Leelavinothan?PariEmail author 《BMC complementary and alternative medicine》2005,5(1):14
Background
This study was undertaken to investigation the effect of Diasulin, a poly herbal drug composed of ethanolic extract of ten medicinal plants on blood glucose, plasma insulin, tissue lipid profile, and lipidperoxidation in alloxan induced diabetes. 相似文献4.
Faridimehr Sina Venkatachalam Saravanan Chinnam Ratna Babu 《Health care management science》2021,24(3):482-498
Health Care Management Science - An important challenge confronting healthcare is the effective management of access to primary care. Appointment scheduling policies/templates can help strike an... 相似文献
5.
N Murali E S Saravanan V J Ramesh B N Gangadhar N Jananakiramiah S S Kumar R Christopher D K Subbakrishna 《Anesthesia and analgesia》1999,89(5):1301-1304
The electroconvulsive therapy (ECT) guideline of the Royal College of Psychiatrists recommends a 0.5 mg/kg of succinylcholine for ECT modification. Our clinical experience suggests that this dose is insufficient for Indian patients. The dose recommended by the Royal College of Psychiatrists (0.5 mg/kg) and a larger dose (1 mg/kg) were compared in 50 patients referred for ECT. In one ECT session, patients were equally randomized to receive one of the two doses and in the next session they were switched to the other dose. The extent of motor seizure modification was rated on a five-point scale by two independent raters who were blinded to the succinylcholine dose. The interrater reliability was good (K = 0.85). "Poor" seizure modification occurred in 48% and 12% of patients with the 0.5 and 1 mg/kg doses, respectively. Of the 24 patients who had poor modification with 0.5 mg/kg, 20 had "good" modification in the session with 1 mg/kg (P < 0.001). A small delay (mean = 55 s) occurred in time to recover from the respiratory paralysis with the 1 mg/kg dose of succinylcholine. No patient, however, had prolonged apnea requiring special measures. We recommend 1 mg/kg of succinylcholine dose be used in the first ECT session. For subsequent sessions, the dose may be altered, depending on the response for optimal motor seizure modification. IMPLICATIONS: The dose of muscle relaxant (succinylcholine) recommended in modified electroconvulsive therapy is not based on empirical research. In the same patients (n = 50), two doses-0.5 mg/kg and 1 mg/kg-were compared during different electroconvulsive therapy sessions. The larger dose was more effective in modifying the peripheral convulsion. 相似文献
6.
Purpose:To assess the level of knowledge, attitude, and clinical practice of glaucoma among optometry students and optometry practitioners with different years of clinical experience and academic background.Methods:A survey with 20 questions on knowledge, attitude, and practice (KAP) of glaucoma was prepared and self-administered to optometry students and optometry practitioners practicing in an eye hospital/clinic/optical with varied years of clinical experience and education qualification.Results:Among the 558 participants, 57% were optometry practitioners and 43% were students. The knowledge scores among optometry practitioners increased significantly with an increase in the years of clinical experience (P < 0.001). Participants with master’s degrees scored higher than participants with bachelor’s degrees (P = 0.12). There was no statistically significant difference in knowledge scores based on the type of clinical practice - hospital, private practice, or optical (P = 0.39). Practicing optometrists who performed slit-lamp examination, gonioscopy, IOP measurements, and disc evaluation for the detection of glaucoma had significantly higher knowledge scores than those who did not perform these tests in their practice (P < 0.05). A positive attitude toward glaucoma learning through workshops and hands-on training was reported by optometrists and students.Conclusion:Knowledge about glaucoma was good among optometrists and optometry students and was better among those who handled the diagnostics. All the optometrists had a positive attitude toward enhancing their practice through proper training. 相似文献
7.
Ravi Mahalingam Brittany Feia Colin Coleman Kusala Anupindi Pratush Saravanan Amalia Luthens Amalia Bustillos Arpita Das Eileen de Haro Lara Doyle-Meyers Jayme Looper Andrew N. Bubak Christy S. Niemeyer Brent Palmer Maria A. Nagel Vicki Traina-Dorge 《Viruses》2022,14(6)
Primary simian varicella virus (SVV) infection and reactivation in nonhuman primates is a valuable animal model in the study of varicella zoster virus disease [varicella (chickenpox) and herpes zoster (shingles)]. To understand SVV pathogenesis in skin, we inoculated 10 rhesus macaques with SVV, resulting in varicella rash. After the establishment of latency, eight of the monkeys were immunosuppressed using tacrolimus with or without irradiation and prednisone and two monkeys were not immunosuppressed. Zoster rash developed in all immunosuppressed monkeys and in one non-immunosuppressed monkey. Five monkeys had recurrent zoster. During varicella and zoster, SVV DNA in skin scrapings ranged from 50 to 107 copies/100 ng of total DNA and 2–127 copies/100 ng of total DNA, respectively. Detection of SVV DNA in blood during varicella was more frequent and abundant compared to that of zoster. During varicella and zoster, SVV antigens colocalized with neurons expressing β-III tubulin in epidermis, hair follicles, and sweat glands, suggesting axonal transport of the virus. Together, we have demonstrated that both SVV DNA and antigens can be detected in skin lesions during varicella and zoster, providing the basis for further studies on SVV skin pathogenesis, including immune responses and mechanisms of peripheral spread. 相似文献
8.
S. Ravikumar M. Gnanadesigan A. Saravanan N. Monisha V. Brindha S. Muthumari 《Asian Pacific journal of tropical medicine》2012,5(11):887-890
ObjectiveTo identify the antibacterial potential of seagrass (Syringodium isoetifolium) associate microbes against bacterial pathogens.MethodsEumeration of microbial associates were analyzed with leaf and root samples of Syringodium isoetifolium. MIC and MBC were calculated for bacterial pathogens with microbial associates. Phylogenetic and GC-MS analysis were calculated for Actinomycetes sp. (Act01) which was the most potent.ResultsOf the isolated microbial associates phosphatase producing bacterial isolates were identified as maximum [(261.78±35.09) CFU×104/g] counts in root sample. Of the selected microbial isolates Actinomycete sp (Act01) showed broad spectrum of antibacterial activity against antibiotic resistant and fish bacterial pathogens. Phylogenetic analysis of Act01 showed maximum identities (99%) with the Streptomyces sp. (GU5500072). The 16s rDNA secondary structure of Act01 showed the free energy values as ?366.3 kkal/mol. The GC-MS analysis Act01 showed maximum retention value with 23.742 RT and the corresponding chemical class was identified as 1, 4-dihydroxy-2-(3-hydroxybutyl)-9, 10-anthraquinone 9, 10-anthrac.ConclusionsIn conclusion, Streptomyces sp. (GU045544.1) from Syringodium isoetifolium could be used as potential antibacterial agent. 相似文献
9.
Transmission of "a" determinant variants of hepatitis B virus in immunized babies born to HBsAg carrier mothers 总被引:1,自引:0,他引:1
Velu V Saravanan S Nandakumar S Dhevahi E Shankar EM Murugavel KG Kumarasamy T Thyagarajan SP 《Japanese journal of infectious diseases》2008,61(1):73-76
Hepatitis B virus (HBV) surface antigen mutations may lead to immune escape and eventually cause failure of immunization. In this report, we identified immune escape variants in immunized babies born to hepatitis B surface antigen (HBsAg) carrier mothers. A total of 68 babies were followed up for 2 years after the full course of vaccination; 2.9% (2/68) of babies were found to be infected with the variant HBV in spite of preexisting antibody to surface antigen (anti-HBs) at 24 months post immunization. Both infants were positive for HBV-DNA; sequencing results of the "a" determinant region of the surface gene revealed that both babies had point mutations at a different nucleotide position resulting in various amino acid substitutions. In addition, an intriguing variant having an addition-deletion mutation was observed in one of the babies. This is the first report to show the addition-deletion variant of HBV in India. However, the immunological significance of the above HBV variants needs to be further elucidated. 相似文献
10.
G. Ramalingam K. Venkata Saravanan T. Kayal Vizhi M. Rajkumar Kathirvelu Baskar 《RSC advances》2018,8(16):8516
Many synthesized semiconductor QDs materials are formed using trioctylphosphine oxide (TOPO) but it requires high temperature, is very expensive and is also hydrophobic. Our study deals with selective syntheses of CdSe and core–shell CdSe/ZnS quantum dots (QDs) in aqueous solution by a simple heating and refluxing method. It is more hydrophilic, needs less temperature, is economically viable and is eco-friendly. Bio-ligands, such as thioacetamide, itaconic acid and glutathione, were used as stabilizers for the biosynthesis of QDs. A simplified aqueous route was used to improve the quality of the colloidal nanocrystals. As a result, highly monodisperse, photoluminescent and biocompatible nanoparticles were obtained. The synthesized QDs were characterized by XRD, FTIR, confocal microscopy, ultraviolet (UV) absorption and photoluminescence (PL). The size of synthesized QDs was observed as 5.74 nm and the core–shell shape was confirmed by using XRD and confocal microscopy respectively. The QD nanoparticles showed antibacterial activity against pathogenic bacteria. The QDs could be applied for biological labelling, fluorescence bio-sensing and bio-imaging etc.Mystristic capped CdSe QDs with schematic diagram and formation mechanism of bio-taggable CdSe@ZnS QDs. 相似文献