指甲延长术在指尖损伤中的临床应用

目的总结指甲延长术在指尖损伤中的临床应用经验。方法用这种方法对8例（拇指3例,食指4例,中指1例）指尖损伤的患者进行了指甲延长术。在距甲根皮缘0.5～0.6cm处,去除一块矩形皮肤,勿损伤皮下血管网,其高度0.2～0.3cm,宽度与甲相等,将“U”形皮瓣向近端柔和推剥并缝合。结果1例术后供区发生表浅感染,经交换敷料逐渐愈合。所有甲延长的手指术后经过顺利,随访7个月～2年（平均13个月）,指甲外形较好,取得较满意的临床效果。结论在指尖损伤中应用指甲延长术,可延长指甲2～3mm,改善了手指的外形,没有发生甲生长畸形,是一种简单有效的指甲延长手术方法。     

Ⅰ期趾甲延长术9例报告

目的:总结Ⅰ期在第2足趾游离移植再造拇(手)指中行趾甲延长的临床应用经验.方法:采用趾甲延长方法对9例(男7,女2例)第2足趾移植再造拇(手)指的患者进行了趾甲延长术,其中拇指8例,食指1例.年龄18～46岁,平均25岁.在再造指距甲根皮缘5 mm处,去除1块矩形皮肤,勿损伤皮下血管网.其高度2 mm,宽度与趾甲相等,将U形皮瓣向近端柔和推剥并缝合.结果:1例术后供区发生表浅感染,经换敷料逐渐愈合.再造的拇(手)指全部成活,可延长趾甲2～3 mm,改善了再造拇(手)指的外形,无指甲生长畸形发生.随访7个月～2年(平均13个月),趾甲外形较好.结论:在第2足趾游离移植再造拇(手)指中应用Ⅰ期趾甲延长术,使趾甲从短小向纵向延长,缩小手指甲与足趾甲之间差异,能改善再造拇(手)指甲外形,且不影响再造指的活动功能,是一种简单有效的手术方法.     

烧伤兔血清对培养内皮细胞的影响

本实验采用血管内皮细胞培养模型，动态观察了家兔烧伤（３０％Ⅲ度）早期烧伤表对内皮细胞结构和功能的影响，通过相差显微动态观察、台盘菜染色、ＨＥ染色光镜检查及扫描电镜观察。同时检测孵育不同时相点增减液中ＬＤＨ、６－ｋｅｔｏ－ＰＧＥ１α、ｔＰＡ及内皮细胞抗上板粘附能力变化。结果显示：（１）烧伤血清对内皮细胞有明显的损伤作用功能改变早于结构损害，而结构损害又以细胞间连接及其表达结构改变为早；（２）烧伤血     

Identification of cultured male epidermal allografts by detecting Y chromosome DNA fragments in female burn victims

ltiswellknownthattheskinofextensivelyburnedcasesthemselvesisinsufficienttoprovidecoverageforwounds.Thoughallogeneicskincancoverthewoundstemporari1y,itwillberejectedwithin2-3weeks.Thiscanthreatenthepatient'slifeifthereisnoenoughautogenousskintoreplacetherejectedoneontime.GreenetalL1]reportedthattheburnwoundsofpatientswithextensiveburnscouldbetreatedbytransplantingepidermalautograftsafterautologousepidermalcellsmulti-plyforthousandsoftimesinvltrowithinashortperiodoftime-Butittakesatleast2-3weeks…     

烧伤血清刺激对大鼠肠上皮细胞结构和粘弹性的影响

目的　动态观察烧伤血清对体外肠上皮细胞 (IEC)骨架和细胞生物力学 (粘弹性 )的影响。　方法　培养大鼠肠上皮细胞株IEC 6 ,用烧伤血清刺激后 ,通过细胞骨架免疫组化、细胞ELISA法定量分析以及粘弹性测定技术 ,动态观察IEC致伤前后的变化。　结果　IEC在烧伤血清作用早期 ,骨架蛋白的表达即明显降低 ,微丝、微管蛋白阳性信号减弱 ,细胞粘弹性下降。　结论　细胞骨架的损伤可引起细胞脆性增加、粘弹性下降 ,导致细胞生物力学特性的改变。这种变化 ,可能直接参与烧伤后肠上皮细胞损伤的发生。     

大面积Ⅲ度烧伤一次性切痂治疗的探讨

目的：探索大面积Ⅲ度烧伤一次性切痂手术的可行性，方法：１０例烧伤病人（总面积３５％～８５％，Ⅱ度２０％～６０％）术前置Ｓｗａｎ－Ｇａｎｚ导管，于血流动力，血气等动态监测下，一次将Ⅱ度烧务面创面全部切除，用异体和（或）自体皮全覆盖，结果：最大切面积６０％最大手术面积（切痂及供皮）达７１％，术中血流动及组织氧合指标短时间降低，术后迅速回升，２４ｈ多可恢复，术后均未发生内脏并发症，１０例均治愈，平均住字     

烧伤大鼠血清对血管内皮细胞通透性的影响

加深入阐明严重烧伤早期全身血管通透性增加继而引直民早期损害的发生机制，本实验应用培养于微孔滤膜上的小牛瞌 及皮细胞单层所构成的选择性通透屏障模型，观察了３０％Ⅲ度烧伤大鼠血清对其影响，结果发现，烧伤早期烧伤血清刺激后ＥＣ单层对Ｈａｎｋｓ液或白蛋白液滤过流量、滤过系数均明显增加。     

Whole CanA Gene Amplification of Helicobacter pylori and Its Fingerprinting by Restriction Fragment Length Polymorphism

To set up a method of amplification for the whole CagA gene of Helicobacter pylori and its fingerprinting by restriction fragment length polymorphism(RFLP),nested PCR was employed in combination with TD-PCR to amplify the gene and EcoRI and Hind Ⅲ were used to generate the RFLP fingerprinting.Target DNA fragments from 13 of 20 samples were successfully amplified and the relevant RFLP fingerprintings were obtained.It is concluded that the method can be used to amplify the whole CagA gene and CagA gene has apparent diversity of RFLP profile.     

四君子汤对烧伤豚鼠早期代谢营养的影响

为探讨四君子汤对烧伤早期代谢营养的作用，采用３０％Ⅲ度烧伤豚鼠胃造口模型将４００ｇ左右豚鼠分别在喂养等量热卡、氮和自由进食两种膳食条件下，将３１只豚鼠分为四组，即喂养治疗组（ｎ＝７），喂养对照组（ｎ＝４），自食治疗组（ｎ＝９），自食对照组（ｎ＝１１）予以观察。结果发现：自由进食时，四君子汤能增加摄食量，减轻伤后体重丢失，改善氮平衡，增加肝、回肠粘膜含氮量，而躯壳重及腓肠肌含氮量增加不明显，但静息代谢率则增加；等量喂养时，四君子汤治疗组体重、躯壳重及腓肠肌含氮量显著低于对照组，而肝、肠粘膜重量及含氮量差异无显著意义。此外，四君子汤还可调整激素和内脏组织含水量。因此，我们认为四君子汤对改善烧伤后代谢营养及脏器功能可能是有益的     

Reconstruction of distal phalanx defect with microsurgical fascia latae flaps combined with rib and skin graft]

OBJECTIVE: To evaluate the clinical application of microsurgical fascia latae flaps combined with rib and skin graft for reconstruction of the distal phalanx defect. METHODS: The phalanx wounds were sutured together like syndactyly. The autologeous rib was revised to repair the bone defect of distal phalanx. The fascia latae flap was used to cover the bone exposure with microvascular anastomoses and resurfaced by a meshed split-thickness skin graft. RESULTS: 5 cases were treated successfully. The fascia latae flaps were all survived with only skin graft necrosis at the distal end in one case. It was healed spontaneously. CONCLUSIONS: The fascia latae flap is nourished by the desending branch of the lateral circumflex femoral artery. The flaps has a good blood supply and can be easily obtained with a long vascular pedicle. The flap is ideal for the reconstruction of distal phalanx defect when combined with autologeous rib implant and skin graft.