Spectrophotometric determination of isonaphthazarin impurity in 1,4-dioxo-2,2,3,3-tetrahydroxy-1,2,3,4-terahydronaphthalene (tetraxolin)

A quantitative spectrophotometric method has been developed for determining the impurity of isonaphthazarin in samples of tetraxolin. The relative error of determination is about 1.0% at a confidence probability of P = 0.95%. The procedure is recommended for the quantitative control of the possible impurity of isonaphthazarin in the parent substance of tetraxolin. __________ Translated from Khimiko-Farmatsevticheskii Zhurnal, Vol. 41, No. 1, pp. 38–40, January, 2007.     

Sperm chromatin integrity in young men with no experiences of infertility and men from idiopathic infertility couples

Damage to the genetic component of spermatozoa seems to play the main role in a majority of cases where current approaches fail to reveal the specific cause of male infertility. In this study, we compared semen quality in men assigned to two defined groups: men from couples with unexplained infertility – idiopathic infertility (A) and young men with no experiences of infertility (B). All samples were examined by standard ejaculate analysis and sperm chromatin structure assay (SCSA). Sperm chromatin damage was significantly higher in men from group A than in those from group B. Similar results were obtained by comparison of men from group A (all men were normozoospermic) with normozoospermic men from group B. According to these results, we can suppose that chromatin disorders may be the causal factor of subfertility or infertility in some of these men. No evidence for a strong association between chromatin disorders and standard parameters of ejaculates was found. We failed to confirm a relationship between smoking and sperm quality in men from any of the investigated groups. SCSA is a method that facilitates the identification of infertile men who otherwise show normal semen variables.     

Diagnostic value of studying the indicators of blood lipid composition and the status of intravascular microcirculation in patients with diabetes mellitus associated with ischemic heart disease

A comprehensive study of blood lipid composition and microcirculatory indices was performed in 88 patients with diabetes mellitus (DM) combined with coronary heart disease (CHD). A "syndrome" of mutual aggravation was shown to develop in the combination of DM and CHD. As a result of it the above indices were changed depending on clinical signs of each of the two diseases. Lipid metabolic and microcirculatory derangements depended mostly on a degree of compensation of carbohydrate metabolism. a DM type, the presence of diabetic angiopathies and CHD gravity.     

Morphological analysis of the formation of cluster organization of neurons,forming the corticocortical connections in the cat visual cortex in early postnatal ontogenesis

The development of cluster organization of neurons forming direct interzonal associative projections from striate cortex (area 17) to extrastriate area 21a in cat was studied by a technique of horseradish peroxidase retrograde transport. Reconstruction of cortical region containing retrogradely labeled neurons was performed using continuous serial sections of the brain. The analysis of spatial organization was done and quantitative characteristics of distribution pattern of initial corticocortical neurons in striate cortex in kittens were obtained. Interzonal connections were detected since 14th postnatal day. Neuronal cluster organization started forming after day 21 while its pattern became similar to that in the adult animal both qualitatively and quantitatively by the end of the 2nd postnatal month, that is somewhat later than it was previously thought. The data obtained also demonstrate the age-dependent decrease in the convergence of the striate cortex projections to area 21a associated with the reduction of the area, occupied by initial neurons.     

Changes in agent variability and time course of disease incidence during a year (as exemplified by dysentery)

The vulnerability of epidemic process during the period of minimum annual incidence of the disease is validated. Biological properties of Shigella sonnei are studied and their variability examined using the index for evaluation of the mean number of variations for a sign. Minimum agent heterogeneity coincides with minimum incidence of disease and maximum heterogeneity with its seasonal rises.