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Due to demographic change and increase of dementia there is an obvious need of voluntary hospice helpers who support dementia patients. The aim of this qualitative study was to describe the experience and challenges of hospice volunteers supporting dementia patients. The results were taken into account in the development of a training course. Ten narrative interviews were conducted with voluntary hospice helpers. The audiotaped interviews were analysed according to an interpretative phenomenological method. Three main issues, each consisting of three catagories, emerged from the interview data. The voluntary hospice helpers experience different facets and the progress of dementia ("to experience the process"). They provide a pleasant change in everyday life of and express their solidarity with dementia patients ("to create a good atmosphere"). They empathetically support dementia patients without expecting anything in return and they benefit from their work with dying people ("to put one's own interests last"). Voluntary hospice helpers offer important support for dementia patients but their role in the interdisciplinary team has to be defined. Training courses are useful to improve interaction and reflection skills. 相似文献
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Induction of aneuploidy by increasing chromosomal instability during dedifferentiation of hepatocellular carcinoma
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Wilkens L Flemming P Gebel M Bleck J Terkamp C Wingen L Kreipe H Schlegelberger B 《Proceedings of the National Academy of Sciences of the United States of America》2004,101(5):1309-1314
To gain more insight into the role of chromosomal instability (CIN), the cytogenetic hallmark of most solid tumors, we performed fluorescence in situ hybridization (FISH) on interphase nuclei of cytological specimens enabling the correct detection of chromosome copies in intact tumor cells of 18 well (G1), moderately (G2), or poorly (G3) differentiated hepatocellular carcinomas (HCCs). A close correlation between the morphological dedifferentiation and increasing copy numbers and variation of FISH signals was seen for chromosomes 1 and 8, respectively (P < or = 0.0002). Four HCC G1 had constant chromosome patterns for chromosomes 1 and/or 8 with a mean of signals per nucleus < or =5.08 and < or =3 different signal combinations, indicating a low level of CIN, as confirmed by FISH using probes for centromeres of chromosomes 3, 7, and 17. In contrast to this, five HCC G2-3 revealed > or =8.46 signals per nucleus and 23-41 different signal combinations, indicating high levels of CIN. In the remaining cases, signal counts from 5.96-8.46 and 7-15 combinations were seen. Here, nuclei with constant aberration patterns and low copy numbers occurred alongside nuclei with inconstant patterns and high copy numbers. It is evident that in these cases a transition from well to moderately differentiated HCC developed in parallel to an increase in CIN, possibly induced by a major dysregulation of mitotic control mechanisms. In conclusion, CIN may induce a stepwise increase of aneuploidy in HCC that is mirrored by the morphological dedifferentiation of tumor cells. 相似文献
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Long‐term follow up of 3 T MRI‐detected brain lesions after percutaneous catheter‐based left atrial appendage closure
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Barbara Bellmann MD Andreas Rillig MD FEHRA Carsten Skurk MD David Manuel Leistner MD Karl Georg Haeusler MD FESC Tina Lin MBBS BMedSci FRACP Rohat Geran MD Luzie Koehler MD Selma Guttmann Verena Tscholl MD Mattias Roser MD Klaus Lenz Kersten Villringer MD Jai‐ Wun Park MD Jochen B. Fiebach MD Ulf Landmesser MD FESC 《Catheterization and cardiovascular interventions》2018,92(2):327-333
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Ferdinand O. Bohmann Katharina Gruber Natalia Kurka Laurent M. Willems Eva Herrmann Richard du Mesnil de Rochemont Peter Scholz Heike Rai Philipp Zickler Michael Ertl Ansgar Berlis Sven Poli Annerose Mengel Peter Ringleb Simon Nagel Johannes Pfaff Frank A. Wollenweber Lars Kellert Moriz Herzberg Luzie Koehler Karl Georg Haeusler Anna Alegiani Charlotte Schubert Caspar Brekenfeld Christopher E. J. Doppler
zgür A. Onur Christoph Kabbasch Tanja Manser Helmuth Steinmetz Waltraud Pfeilschifter 《European journal of neurology》2022,29(1):138-148
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Chronic obstructive pulmonary disease (COPD) is a progressive disorder including chronic obstructive bronchitis, emphysema and combinations. The management of stable COPD in an advanced stage involves noninvasive mechanical ventilation. There is a lack of convincing scientific studies about the influence of mechanical ventilation on quality of life. Furthermore the role of nurses taking care of people with COPD seems to be unclear. A phenomenological interpretative-hermeneutical analysis of 15 narrative interviews with non-invasive ventilated COPD-patients demonstrates that noninvasive ventilation is associated with improvements in patients' autonomy. "To achieve independence" is described as the central theme of this study and highlights the benefit of independency on patients' quality of life. Other results describe the experiences "To experience breathing", "To seize one's possibilities", "To participate in life", "To have to live with COPD". The results also show that activities of nurses in particular need to include consulting, teaching and monitoring. There is a need for further research to evaluate nursing interventions. This study is a first step in knowledge generation and serves as a basis for further quantitative research. 相似文献
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Doris Steinemann Britta Skawran Thomas Becker Marcel Tauscher Anja Weigmann Luzie Wingen Sarah Tauscher Tanja Hinrichsen Sabine Hertz Peer Flemming Jacobus Flik Birgit Wiese Hans Kreipe Peter Lichter Brigitte Schlegelberger Ludwig Wilkens 《Clinical gastroenterology and hepatology》2006,4(10):1283-1291
BACKGROUND & AIMS: To gain more information about the molecular mechanisms leading to dedifferentiation of hepatocellular adenoma (HCA) and hepatocellular carcinoma (HCC), high-resolution array-based comparative genomic hybridization (array-CGH) was performed on 24 cases of HCC and 10 cases of HCA. METHODS: DNA chips containing 6251 individual bacterial artificial chromosome/plasmid artificial chromosome clones were used. They allowed for a genome-wide resolution of 1 Mb and an even higher resolution of up to 100 kb for chromosome regions recurrently involved in human tumors and for regions containing known tumor-suppressor genes and oncogenes. RESULTS: Copy number changes on the genomic scale were found by array-based comparative genomic hybridization in all cases. In HCC, gains of chromosomal regions 1q (91.6%), and 8q (58.3%), and losses of 8p (54%) were found most frequently. Hierarchic cluster analysis branched all HCA from HCC. However, in 2 adenomas with a known history of glycogenosis type I and adenomatosis hepatis gains of 1q were found, too. The critically gained region was narrowed down to bands 1q22-23. Although no significant differences in the mean number of chromosomal aberrations were seen between adenomas and well-differentiated carcinomas (2.7 vs 4.6), a significant increase accompanied the dedifferentiation of HCC (14.1 in HCC-G2 and 16.3 in HCC-G2/3; P < .02). Dedifferentiation of HCC also was correlated closely to losses of 4q and 13q (P <.001 and <.005, respectively). CONCLUSIONS: The increased chromosomal instability during dedifferentiation of HCC leads to an accumulation of structural chromosomal aberrations and losses and gains of defined chromosome regions. 相似文献
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Epigenetic defects of hepatocellular carcinoma are already found in non-neoplastic liver cells from patients with hereditary haemochromatosis 总被引:3,自引:0,他引:3
Lehmann U Wingen LU Brakensiek K Wedemeyer H Becker T Heim A Metzig K Hasemeier B Kreipe H Flemming P 《Human molecular genetics》2007,16(11):1335-1342
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Wingen LU Münster T Faigl W Deleu W Sommer H Saedler H Theißen G 《Proceedings of the National Academy of Sciences of the United States of America》2012,109(18):7115-7120
Pod corn is a classic morphological mutant of maize in which the mature kernels of the cob are covered by glumes, in contrast to generally grown maize varieties in which kernels are naked. Pod corn, known since pre-Columbian times, is the result of a dominant gain-of-function mutation at the Tunicate (Tu) locus. Some classic articles of 20th century maize genetics reported that the mutant Tu locus is complex, but molecular details remained elusive. Here, we show that pod corn is caused by a cis-regulatory mutation and duplication of the ZMM19 MADS-box gene. Although the WT locus contains a single-copy gene that is expressed in vegetative organs only, mutation and duplication of ZMM19 in Tu lead to ectopic expression of the gene in the inflorescences, thus conferring vegetative traits to reproductive organs. 相似文献
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Distinct methylation patterns of benign and malignant liver tumors revealed by quantitative methylation profiling. 总被引:8,自引:0,他引:8
Ulrich Lehmann Ina Berg-Ribbe Luzie U Wingen Kai Brakensiek Thomas Becker Jürgen Klempnauer Brigitte Schlegelberger Hans Kreipe Peer Flemming 《Clinical cancer research》2005,11(10):3654-3660
PURPOSE: A comparative quantitative methylation profiling of hepatocellular carcinoma and the most frequent benign liver tumor, hepatocellular adenoma, was set up for the identification of tumor-specific methylation patterns. EXPERIMENTAL DESIGN: The quantitative methylation levels of nine genes (RASSF1A, cyclinD2, p16INK4a, DAP-K, APC, RIZ-1, HIN-1, GSTpi1, SOCS-1) were analyzed in hepatocellular carcinoma and adjacent normal tissue (n = 41), hepatocellular adenoma and adjacent normal tissue (n = 26), focal nodular hyperplasia (n = 10), and unrelated normal liver tissue (n = 28). Accumulated methylation data were analyzed using various statistical algorithms, including hierarchical clustering, to detect tumor-specific methylation patterns. RESULTS: Cluster analysis revealed that hepatocellular adenoma displays a methylation profile much more similar to that found in normal liver tissue and focal nodular hyperplasia than to that found in hepatocellular carcinoma. Many characteristic differences were not detected when using mere qualitative methylation assays. The cyclinD2 gene was identified as a new and frequent target for aberrant hypermethylation in hepatocellular carcinoma (68%). In the control group of 28 liver specimens from healthy donors, a clear correlation between age of patient and frequency and level of aberrant methylation was seen, which could not be detected in the group of hepatocellular carcinoma specimens. CONCLUSIONS: Methylation profiling can clearly contribute to the unequivocal classification of suspicious lesions, but only if done in a quantitative manner applying cell type and gene-specific thresholds. In hepatocellular carcinoma, the altered methylation patterns accompanying malignant transformation override the age-dependent increase in gene methylation. 相似文献