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排序方式: 共有830条查询结果,搜索用时 46 毫秒
1.
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Vitreous clouding associated with Lyme borreliosis 总被引:1,自引:0,他引:1
3.
M J Jongen S Kuiper W J van der Vijgh P Lips J C Netelenbos 《Zeitschrift für klinische Chemie und klinische Biochemie》1988,26(1):25-28
Technical improvements have been applied to reduce the time required for the determination of calcidiol and calcitriol in serum or plasma. The modifications include the use of Sep-Pak C18 cartridges for the extraction of calciol metabolites from serum instead of a classical liquid/liquid extraction, a considerable shortening of the HPLC purification time compared with our previously described method, and the application of HPLC with UV detection at 254 nm of calcidiol as an alternative to the usual competitive protein binding methods. In none of the 199 samples where calcidiol was determined by HPLC did we observe a detectable peak of ercalcidiol. Quantitation of calcidiol by HPLC and competitive protein binding was compared in 5 series of assays. The correlation between the two methods was 0.99. The average slope of the linear regression line when the HPLC values were plotted versus the competitive protein binding values was 1.14. The average intercept was 0.19 nmol/l. The mean within-run coefficient of variation for calcidiol in these series was 5% for the competitive protein binding method, and 4% for HPLC method. Between-run coefficients of variation were 6% and 12% for the competitive protein binding and for the HPLC method, respectively. Within-run and between-run coefficients of variation for calcitriol were 6% and 15%, respectively. 相似文献
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Ethics roundtable debate: Patients and surrogates want 'everything done' – what does 'everything' mean?
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![点击此处可从《Critical care (London, England)》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Christopher Doig Holt Murray Rinaldo Bellomo Michael Kuiper Rubens Costa Elie Azoulay David Crippen 《Critical care (London, England)》2006,10(5):231
Highly complex and specialized care plans sometimes overwhelm the comprehension of patients and families. Many optimistic surrogates of critically ill patients err on the side of desiring that everything be done but with a nebulous idea of what 'everything' entails. Physicians must work closely to educate surrogates as to the benefits versus the risks of treatment. Our roundtable experts ponder the question of whether providers possess the authority to interpret unilaterally the nature of requests for everything. 相似文献
6.
L A Hoogenboom M C Berghmans A Van Veldhuizen H A Kuiper 《Drug metabolism and disposition》1990,18(6):999-1004
Porcine hepatocytes were used to examine the biotransformation of beta-nortestosterone (NOR). Initially, the major metabolite of NOR was norandrostenedione (NA), which upon prolonged incubation was further transformed, primarily to the glucuronide of 15 alpha-hydroxy-norandrostenedione (15 alpha OH-NA). No differences were observed in this pattern between hepatocytes isolated from livers of sows or castrated male pigs. With increasing culture age, the rate of formation of NA did not change, contrary to a decrease in its further oxidation and glucuronidation. 15 alpha-OH-NA, primarily as its glucuronide, was identified in the urine of pigs injected with NOR. In addition, NA and the glucuronide of the parent compound were present in much smaller amounts. 相似文献
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George G. J. M. Kuiper Petra E. de Ruiter J. Anton Grootegoed Albert O. Brinkmann 《Molecular and cellular endocrinology》1991,80(1-3):65-73
Androgen receptor synthesis and modification were studied in the human LNCaP cell line. Immunoblotting with a specific polyclonal antibody showed that the androgen receptor migrated as a closely spaced 110–112 kDa doublet on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels. Most of the receptor protein is present in the higher molecular mass form. Pulse labelling experiments with [35S]methionine showed that the androgen receptor is synthesized as a single 110 kDa protein which is rapidly converted to a 112 kDa protein. Alkaline phosphatase treatment of cytosols from [35S]methionine pulse labelled cells caused a gradual elimination of the 112 kDa isoform with a concomitant increase of the 110 kDa isoform. This indicates that the observed 110 to 112 kDa upshift of the newly synthesized androgen receptor reflects receptor phosphorylation. Both isoforms can bind hormone and can undergo a hormone dependent transformation to a tight nuclear binding form, indicating that the 110 to 112 kDa conversion is not an obligatory step for hormone binding or receptor transformation. 相似文献
9.
Stress distribution around dental implants: influence of superstructure, length of implants, and height of mandible. 总被引:21,自引:0,他引:21
The stress distribution around dental implants was investigated by use of a two-dimensional model of the mandible with two implants. A vertical load of 100 N was imposed on abutments or the bar connection. The stress was calculated for a number of superstructures under different loading conditions with the help of the finite element method. The length of the implants and the height of the mandible were also varied. A model with solitary abutments showed a more uniform distribution of the stress when compared with a model with connected abutments. The largest compressive stress was also less in the model without the bar. Using shorter implants did not have a large influence on the stress around the implants. When the height of the mandible was reduced, a substantially larger stress was found in the bone around the implants because of a larger overall deformation of the lower jaw. 相似文献
10.
Gastric and duodenal mucosa in ''healthy'' individuals. An endoscopic and histopathological study of 50 volunteers.
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![点击此处可从《Journal of clinical pathology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
The results of histological and immunohistochemical examination of gastric and duodenal biopsy specimens from 50 volunteers without a clinical history of gastrointestinal disease are reported. Multiple specimens of tissue from standard sites in the stomach and duodenum were carefully orientated, and serially sectioned for examination by light microscopy and for immunohistochemical characterisation of plasma cells within the lamina propria. The antrum and fundus were normal in 32 of the 50 subjects but the other 18 showed histopathological evidence of gastritis in either the antrum or fundus. The latter appeared to be age-related. There was considerable variation in the appearance of the surface epithelium of the duodenum within as well as among individual subjects. Superficial gastric metaplasia in one or more biopsy specimens from the duodenal bulb was found in 64% of individuals. Histopathological examination of the duodenum revealed signs of chronic inflammation in 12% of the subjects. In two individuals there was active inflammation but in only one of these was the diagnosis made on endoscopic appearances. Histological criteria important for the diagnosis of duodenitis are discussed. The number of plasma cells in different biopsy specimens from subjects not showing histological signs of inflammation was variable. The ratio IgA:IgG:IgM producing plasma cells was remarkably constant from subject to subject as well as from specimen to specimen. 相似文献