An ion-selective sensor for assay of diclofenac in medicines

A diclofenac-selective electrode with a plasticized polyvinylchloride membrane was developed. The electrode contained an ionic associate of diclofenac with neutral red and its response was linear over the diclofenac concentration range 5 × 10^–5 to 5 × 10^–2 M with an electrode function slope of (30.0 ± 1.1) - (44.0 ± 1.2) mV/pC. This membrane electrode was used as a sensor for assaying diclofenac in pharmaceutical formulations.     

Potentiometric Sensor for Benzylpenicillin Determination

Pharmaceutical Chemistry Journal - A potentiometric benzylpenicillin-sensitive sensor with a plasticized polyvinyl-chloride membrane was developed. The sensor contains a...     

Potentiometric Sensor for Ketoprofen Determination

Pharmaceutical Chemistry Journal - A ketoprofen-sensitive potentiometric sensor with a plasticized PVC membrane was developed. The sensor contained an ion associate of ketoprofen with basic fuxine....     

The oxidative stress in platelets of patients with ovary cancer as observed at chemotherapy

Disorders in the main chains of platelet antioxidant protection were examined in 32 patients with primarily-diagnosed ovary cancer who were postoperatively receiving chemotherapy according to PC. The activity of antioxidant-protection enzymes (superoxide dismutase, catalase, glutation-S-transferase) as well as the content of malonic dialdehyde (MDA) and glutathione were examined after each course of chemotherapy. The data obtained were compared with the aggregation ability of platelets, with the content of fibrinogen and with the count of platelets. The parameters of the antioxidant system in platelets were examined for control in 30 virtually healthy women. The results denote that the oxidant stress progression in the body due to the growing tumor and aggravating because of chemodrugs deregulates the free-radical processes in platelets, which can affect their functional properties or rheological blood properties.     

Immunotoxicological characteristics of preparations based on carcinoembryonic antigen and mucin containing CA 125 antigen

Experiments on male hybrid mice demonstrated that specific immunotherapy with preparations based on carcinoembryonal antigen and mucin containing CA 125 antigen was not associated with general toxicity, local irritating effect, and hepatorenal dysfunction. The absence of toxicity is apparently due to the fact that antigens injected intramuscularly or subcutaneously virtually do not enter the blood. Injections of preparations based on carcinoembryonal antigen and mucin containing CA 125 antigen to mice induced a standard immune response with predominance of class M immunoglobulins during the early terms and class G immunoglobulins at later terms. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 129, No. 4, pp. 462–464, April, 2000     

Effect of extracorporeal sorption on the content of free intracellular calcium of the leukocytes in atopic bronchial asthma

Antigen-IgE-antibody interaction on the cell surface in an allergic reaction increases methylation of phospholipids of a cell membrane making it permeable for Ca2+ ions. Therefore of interest is a study of free intracellular Ca2+ concentration from a view-point of development of atopic bronchial asthma and the use of extracorporal methods of its therapy permitting the elimination of IgE-antibodies from the blood flow. The level of intracellular Ca2+ in the leukocytes of patients with atopic bronchial asthma was studied. It has been shown that 1. the fraction of leukocytes with a higher level of intracellular Ca2+ in patients with atopic bronchial asthma was increased as compared to healthy donors; 2. extracorporal sorption results in a decrease in the number of leukocytes with high Ca concentration.     

Antioxidant system of platelets in patients with ovary cancer

The activities of lipid peroxidation and of platelets' antioxidant protection were investigated in 30 healthy women and in 44 patients with primarily diagnosed different-stage ovary cancer (OC). An essentially higher activity of superoxide dimutase (SOD, by 2.5 times) and of catalase (by 1.5 times) was detected in platelets of OC patients versus the norm. A simultaneous increase of malonic dialdehyde (MDA) by 2.5 times denotes a free-radical activation. There was also an insignificant growth of the glutathione level, whereas the activity of glutathione-S-transferase (GST) did not virtually change. A comparative analysis of the biochemical indices of patients with different stages of the disease revealed valuable distinctions related with a higher activity of SOD and catalase in stages 3-4 versus the initial stages. Notworthility, the MDA level was decreasing. Minor changes in the system of glutathione are not obviously decisive in impairing the regulatory mechanism of antioxidant protection in platelets unlike in malignant cells. The results provide a solid basis for assuming that the impact made by deranged regulations of free radicals on the platelet chain in the hemostasis system causes changes in the blood rheological properties entailing a higher aggregation of platelets, thus, triggering the onset of platelet-embolic complications in the discussed patient category. The impaired regulation mechanism of antioxidant protection in platelets is a major manifestation of the oxidant stress evolving due to a growing tumor; the mechanism is important for evaluating the structural-and-functional platelets' condition.     

Selective Extraction-Photometric Determination of Non-Steroidal Anti-Inflammatory Drugs

Pharmaceutical Chemistry Journal - The possibility of extracting ion associates (IAs) of some non-steroidal anti-inflammatory drugs with the polymethine dye Astrafloxin was studied. The influence...