Plasma proteome profiling of von Hippel-Lindau disease after total and subtotal nephrectomy: A preliminary study

ObjectivesCommon treatment of renal cell carcinoma associated with von Hippel-Lindau (VHL) disease is total (bilateral) or subtotal nephrectomy. Whereas total nephrectomy is associated with absolutely no residual renal function, subtotal nephrectomy frequently leads to chronic kidney disease (CKD) with some residual renal functions. However, molecular mechanisms underlying CKD remain unclear and the diagnosis of CKD is frequently accomplished at its late stage.Design and methodsWe performed a plasma proteomics study to compare the plasma proteome profile of VHL patient who underwent total nephrectomy to the profiles of VHL patient with subtotal nephrectomy and healthy control. Totally 100 μg proteins from each sample was resolved by two-dimensional electrophoresis (2-DE) in triplicate and visualized with SYPRO Ruby fluorescence stain.ResultsThe normal plasma proteome profile markedly differed from the profiles of VHL patients. Comparative analysis between total versus subtotal nephrectomized patients revealed significant differences in levels of 20 plasma proteins. Pathway analysis revealed two important networks involving in lipid metabolism, molecular transport, carbohydrate metabolism, cellular growth and proliferation, and small molecule biochemistry, in which these identified and other proteins interplayed.ConclusionsOur data identified potential biomarkers for CKD. Further characterization of these identified proteins might also lead to better understanding of molecular mechanisms underlying CKD.     

A new phenolic compound from the stem of Hymenocardia wallichii

Thirteen compounds were isolated from the stem of Hymenocardia wallichii Tul. A new phenolic compound, wallinol (1), was found in this plant. The other chemical components were squalene (2), stigmasterol (4), homopterocarpin (5), two triterpenes (3 and 13), two benzaldehyde derivatives (6 and 9), three cinnamyl derivatives (7, 11, and 12), a coumarin (8), and a monoglyceride (10). Their structures were elucidated on the basis of spectroscopic methods including IR, HR-ESI-MS, and 1D and 2D NMR data.     

A new phenolic compound from the stem of Hymenocardia wallichii

Thirteen compounds were isolated from the stem of Hymenocardia wallichii Tul. A new phenolic compound, wallinol (1), was found in this plant. The other chemical components were squalene (2), stigmasterol (4), homopterocarpin (5), two triterpenes (3 and 13), two benzaldehyde derivatives (6 and 9), three cinnamyl derivatives (7, 11, and 12), a coumarin (8), and a monoglyceride (10). Their structures were elucidated on the basis of spectroscopic methods including IR, HR-ESI-MS, and 1D and 2D NMR data.