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排序方式: 共有731条查询结果,搜索用时 15 毫秒
1.
Subcutaneous immunization with a novel immunogenic candidate (urease) confers protection against Brucella abortus and Brucella melitensis infections 下载免费PDF全文
Morteza Abkar Jafar Amani Abbas Sahebghadam Lotfi Gholamreza Nikbakht Brujeni Saeed Alamian Mehdi Kamali 《APMIS : acta pathologica, microbiologica, et immunologica Scandinavica》2015,123(8):667-675
Brucellosis is a world prevalent endemic illness that is transmitted from domestic animals to humans. Brucella spp. exploits urease for survival in the harsh conditions of stomach during the gastrointestinal infection. In this study, we examined the immune response and the protection elicited by using recombinant Brucella urease (rUrease) vaccination in BALB/c mice. The urease gene was cloned in pET28a and the resulting recombinant protein was employed as subunit vaccine. Recombinant protein was administered subcutaneously and intraperitoneally. Dosage reduction was observed with subcutaneous (SC) vaccination when compared with intraperitoneal (IP) vaccination. rUrease induced mixed Th1–Th2 immune responses with high titers of specific IgG1 and IgG2a. In lymphocyte proliferation assay, splenocytes from IP and SC‐vaccinated mice displayed a strong recall proliferative response with high amounts of IL‐4, IL‐12 and IFN‐γ production. Vaccinated mice were challenged with virulent Brucella melitensis, B. abortus and B. suis. The SC vaccination route exhibited a higher degree of protection than IP vaccination (p value ≤ 0.05). Altogether, our results indicated that rUrease could be a useful antigen candidate for the development of subunit vaccines against brucellosis. 相似文献
2.
Successful treatment of chronic idiopathic urticaria and angioedema with cimetidine alone 总被引:1,自引:0,他引:1
Jafar Farnam M.D. J. Andrew Grant M.D. Brock G. Guernsey Pharm. D. Joseph L. Jorizzo M.D. Emil R. Petrusa Ph. D. 《The Journal of allergy and clinical immunology》1984,73(6):842-845
We have studied a 50-year-old white man with chronic urticaria and angioedema who has responded to treatment with cimetidine alone for over 2 yr. In a double-blind, placebo-controlled study, cimetidine alone was at least as effective as chlorpheniramine in relief of urticaria and angioedema. Additionally, cimetidine significantly inhibited (p less than 0.01) the wheal response to histamine when it was compared to placebo. The inhibition of wheal response to histamine by cimetidine was significantly higher (p less than 0.05) than chlorpheniramine. The presence of predominantly H2- rather than H1-histamine receptors in the cutaneous blood vessels may be responsible for the therapeutic effects of cimetidine in this patient. 相似文献
3.
Hamed Nikoupour MD Peyman Arasteh MD MPH Alireza Shamsaeefar MD Fardin Ghanbari MD Arash Boorboor MD Ali Mosa Jafar Almayali MD Mojtaba Shafiekhani PharmD Ph.D Pirouz Samidoust MD Reza Shahriarirad MD Alireza Shojazadeh MD Keivan Ranjbar MD Mohammad Hasan Darabi MD Sina Tangestanipour MD Seyed Morteza Hosseini MD Leila Zahiri MD Saman Nikeghbalian MD 《JPEN. Journal of parenteral and enteral nutrition》2022,46(4):946-957
4.
The experimental model of a cerebral missile injury developed by Crockard was used in three groups of Rhesus monkeys treated with mannitol. One group received mannitol 15 minutes after being injured with a BB pellet at 90 m/sec impact. Another group was wounded identically, but mannitol treatment was delayed until 1 hour after injury. The last group was wounded with the missile traveling at 180 m/sec, and mannitol was started 15 minutes after trauma. The data were contrasted with the results from the original model. After receiving mannitol, all groups showed marked improvement in mean blood pressure, cerebral perfusion pressure, cerebral blood flow, and cerebral metabolic rate of oxygen consumption out of proportion to the degree of reduction in intracranial pressure (ICP). The authors conclude that the therapeutic value of mannitol may, in some injuries, be directly related to its effects on blood flow and metabolism, as well as to its better known effects upon ICP. 相似文献
5.
Metformin (MTF), an effective biguanide and oral antihyperglycemic agent, is utilized to control blood glucose levels in patients with type II diabetes mellitus, and the determination of its concentration in biological fluids is one of the main issues in pharmacology and medicine. In this work, highly luminescent nitrogen-doped graphene quantum dots (N-GQDs) were modified using terbium (Tb3+)–1,10-phenanthroline (Phen) nanoparticles (NPs) to develop a dual-emission ratiometric fluorescent sensor for the determination of MTF in biological samples. The synthesized N-GQDs/Tb–Phen NPs were characterized using different techniques to confirm their physicochemical properties. The N-GQDs/Tb–Phen NPs showed two characteristic emission peaks at 450 nm and 630 nm by exciting at 340 nm that belong to N-GQDs and Tb–Phen NPs, respectively. The results indicated that the emission intensity of both N-GQDs and Tb–Phen NPs enhanced upon interaction with MTF in a concentration-dependent manner. Also, a good linear correlation between the enhanced fluorescence intensity of the system and MTF concentration was observed in the range of 1.0 nM–7.0 μM and the limit of detection (LOD) value obtained was 0.76 nM. In addition, the prepared probe was successfully used for the estimation of MTF concentration in spiked human serum samples. In conclusion, the reported dual-emission ratiometric fluorescent sensor can be used as a sensitive and simple fluorimetric method for the detection of MTF in real samples.Shcematic representation of the MTF detection by an enhancing mechanism. 相似文献
6.
7.
Seyedeh Fatemeh Musavi Ezmareh Kamran Mehrabani Hadi Raeisi Shahraki 《The Turkish journal of gastroenterology》2022,33(6):532
Background: There is little information about comparison of the mortality patterns in different regions. The current study aimed to assess the trend changes of liver cancer mortality in 6 WHO regions.Methods:The mortality rates of liver cancer in the 6 WHO regions during 1990 to 2017 were extracted from the Global Burden of Disease database. Growth mixture model was used to identify the latent patterns using a set of tools to handle both the within and between trajectory variations. All the modeling procedures were implemented in Mplus 7.4 software.Results:The mortality rate in Africa showed a slow decreasing trend throughout the observation period, but a slow increase was observed in the Eastern Mediterranean region, European region, region of the Americas, and South-East Asia region. The slope of the rise in liver cancer mortality rate for Western Pacific region was 3-4 times higher than the other regions. 2-pattern growth mixture model was estimated as the best model. The Western Pacific region with annual increase of 0.20 in the liver cancer mortality rate was in the first pattern and the other 5 regions including Africa region, Eastern Mediterranean region, European region, region of the Americas, and South-East Asia region with annual increase of 0.05 in liver cancer mortality rate belonged to the second pattern.Conclusion:Observed pattern in the Western Pacific region is not favorable. Taking into account a set of urgent prevention actions to control mortality rate of liver cancer in this region seems necessary. 相似文献
8.
OBJECTIVE: The treatment of giant intracranial aneurysms is a challenge because of the limitations and difficulty of direct surgical clipping and endovascular coiling. We describe the indications, surgical technique, and complications of saphenous vein extracranial-to-intracranial bypass grafting followed by acute parent vessel occlusion in the management of these difficult lesions. METHODS: Between January 1990 and December 1999, 29 patients with giant intracranial aneurysms underwent 30 saphenous vein bypass grafts followed by immediate parent vessel occlusion. There were 11 men and 18 women with a mean follow-up period of 62 months. Twenty-five patients harbored aneurysms involving the internal carotid artery, 2 had middle cerebral artery aneurysms, and 2 had aneurysms in the basilar artery. Serial cerebral or magnetic resonance angiograms were obtained to assess graft patency and aneurysm obliteration. RESULTS: All 30 aneurysms were excluded from the cerebral circulation, with 28 vein grafts remaining patent. Two patients had graft occlusions: one because of poor runoff and the other because of misplacement of a cranial pin during a bypass procedure on the contralateral side. Other surgical complications included one death from a large cerebral infarction, homonymous hemianopsia from thrombosis of an anterior choroidal artery after internal carotid artery occlusion, and temporary hemiparesis from a presumed perforator thrombosis adjacent to a basilar aneurysm. CONCLUSION: With appropriate attention to surgical technique, a saphenous vein extracranial-to-intracranial bypass followed by acute parent vessel occlusion is a safe and effective method of treating giant intracranial aneurysms. A high rate of graft patency and adequate cerebral blood flow can be achieved. Thrombosis of perforating arteries caused by altered blood flow hemodynamics after parent vessel occlusion may be a continuing source of complications. 相似文献
9.
Mohammad A. Zmaili Jafar M. Alzubi Duygu Kocyigit Agam Bansal Gursharan S. Samra Richard Grimm Brian P. Griffin Bo Xu 《The American journal of medicine》2021,134(3):361-369
BackgroundNonbacterial thrombotic endocarditis, or marantic endocarditis, is rare. Contemporary data on the etiology, echocardiographic evaluation, and management of nonbacterial thrombotic endocarditis are limited.MethodsA single-center retrospective cohort study was performed. Electronic medical records and echocardiographic records were searched for patients ages ≥18 years with a confirmed diagnosis of nonbacterial thrombotic endocarditis between January 1999 and November 2019. Demographic, echocardiographic, and management data were collected.ResultsOf 600,577 transthoracic echocardiograms (TTEs) and 89,264 transesophageal echocardiograms (TEEs), 42 patients had nonbacterial thrombotic endocarditis (mean age: 54 ± 14.5 years; 66.7% were female). The median duration of follow-up was 8.2 (interquartile range 3.3-24.4) months. Seventeen patients (40.5%) had malignancy, 33.3% had systemic lupus erythematosus, and 35.7% had antiphospholipid antibody syndrome. Stroke was the most common presentation (59.5%).TTE enabled the diagnosis in 19 cases (45.2%), compared with TEE, which identified the condition in 33 of 34 (97.1%) cases in which it was utilized. Three-dimensional echocardiography was performed in 17 TEEs. The most common valves involved were mitral (61.9%), and aortic (23.8%) valves. Thirty-two patients were managed with anticoagulation. Ten patients underwent surgery. Sixteen (38.1%) patients died, most of whom had a diagnosis of advanced malignancy.ConclusionIn a contemporary 20-year cohort, TTE and TEE played important roles in diagnosis, with superior diagnostic performance of TEE for nonbacterial thrombotic endocarditis. Mortality was high, and advanced malignancy portended a worse prognosis. Management in most cases was therapeutic anticoagulation. In select cases, surgery provided favorable outcomes. 相似文献
10.
Mohammad Amin Fallahzadeh Sophia T. Abdehou Jafar Hassanzadeh Fatemeh Fallhzadeh Mohammad Hossein Fallahzadeh Leila Malekmakan 《Indian Journal of Critical Care Medicine》2015,19(6):311-315