细胞周期调控相关蛋白在子宫内膜癌的表达及意义

目的　探讨与细胞周期G1→S调控点相关的P185 ,rasP2 1,P5 3,P16 ,P2 1,Rb ,nm2 3H1蛋白在子宫内膜癌组织中的表达及意义。②方法　应用免疫组化 (LSAB)法检测上述蛋白在 2 1例正常子宫内膜、19例子宫内膜上皮内瘤样病变 (EIN)及 45例子宫内膜癌中的表达。③结果　P185 ,rasP2 1,P5 3蛋白表达率由正常内膜、EIN至内膜癌逐渐升高 ,P16 ,P2 1,Rb ,nm2 3H1结果相反。在子宫内膜癌中 ,P185与rasP2 1呈正相关 (r=0 .36 3,P <0 .0 5 ) ,与P5 3,P2 1呈负相关 (r=- 0 .44 8,- 0 .30 3,P <0 .0 5 ) ,rasP2 1,P5 3与P2 1均呈负相关 (r=- 0 .36 5 ,- 0 .32 2 ,P <0 .0 5 ) ,P16 ,P2 1与Rb呈正相关 (r=0 .36 1,0 .44 1,P <0 .0 5 )。P185单因素分析为一预后保护性因素 (χ2 =5 .86 ,P <0 .0 5 ) ;P5 3的表达与临床各参数均有关 ,与ER ,PR的表达呈负相关性 ,单因素及多因素分析表明 ,P5 3异常表达均提示预后差 (χ2 =2 1.39,P <0 .0 5 )。④结论　与细胞周期G1→S调控相关的P185 ,rasP2 1,P5 3,P16 ,P2 1,Rb ,nm2 3H1蛋白异常表达均参与子宫内膜癌发生、发展 ,且其中部分基因相互关联 ;P5 3可作为独立预后因素 ,其阳性表达提示预后差。     

安脑片联合奥拉西坦治疗脑卒中后血管性痴呆热毒内盛证临床研究

目的：观察安脑片联合奥拉西坦治疗脑卒中后血管性痴呆（VD）热毒内盛证的临床疗效。方法：按随机数字表法将100例脑卒中后VD热毒内盛证患者分为治疗组与对照组,每组50例。对照组采用奥拉西坦治疗,治疗组给予安脑片联合奥拉西坦治疗,2组均治疗4周。比较2组临床疗效与不良反应发生率。采用美国国立卫生研究院卒中量表（NIHSS）、简易智能精神状态检查量表（MMSE）、临床痴呆评定量表（CDR）、日常生活活动能力量表（ADL）评估神经功能、认知功能、痴呆程度和日常生活活动能力;比较双侧大脑中动脉（MCA）、基底动脉（BA）、大脑前动脉（ACA）、椎动脉（VA）平均血流速度,以及血清脑源性神经营养因子（BDNF）、血管内皮生长因子（VEGF）水平。结果：治疗后,治疗组临床疗效总有效率96.00%,高于对照组82.00%(P<0.05)。治疗后,2组NIHSS、CDR评分均较治疗前降低（P<0.05）,MMSE、ADL评分均较治疗前升高（P<0.05）;治疗组NIHSS、CDR评分均低于对照组（P<0.05）,MMSE、ADL评分均高于对照组（P<0.05）。治疗后,2组...     

博宁联合化疗治疗骨转移癌所致疼痛的疗效观察

目的：选择理想的治疗骨转移癌所致疼痛的方法。方法：以博宁联合化疗或以^153Sm—EDTMP内照射联合化疗治疗52例骨转移癌。结果：治疗组总有效率为84．00％，对照组总有效率为59．26％。结论：治疗骨转移癌所致的骨痛，以博宁联合化疗为主的方法优于^153Sm—EDTMP内照射联合化疗的方法。     

Effects of Electroacupuncture on Arrhythmia of Model Rabbits in Different Functional States

目的 探讨电针对不同机能状态家兔心律失常的影响.方法 通过20、25、30 μg/k3种不同剂量的乌头碱,将59只家兔诱发出不同程度的心律失常,以心律失常开始时间和持续时间为指标,对比观察电针对不同机能状态家兔心律失常影响的差异.结果 3种剂量的乌头碱能诱发出家兔3种不同程度的心律失常,三者间差异具有统计学意义；电针对20 μg/kg乌头碱诱发的家兔心律失常的减缓作用明显优于30 μg/kg组.结论 针刺效应与机体异常状态的程度有关,在本实验中,针刺对抗中等程度的家兔心律失常效果最好.     

Heavy metals transported through a multi-trophic food chain influence the energy metabolism and immune responses of Cryptolaemus montrouzieri

Ecotoxicology - Contamination of environment with heavy metals is increasingly becoming an issue of major concern across the globe. Heavy metals are highly toxic to humans as well as other...     

Assessment of glomerular filtration rate in elderly patients with chronic kidney disease

Purpose

We sought to evaluate various glomerular filtration rate (GFR) estimating equations in elderly patients with chronic kidney disease (CKD).

Methods

A total of 332 Chinese elderly patients with CKD who had undergone technetium-99m diethylenetriaminepentaacetic acid (^99mTc-DTPA) renal dynamic imaging were enrolled. The MDRD equation, the re-expressed MDRD equation, the Chinese equation, the Ruijin equation, the previously Japanese equation, the new Japanese equation, the CKD-EPI equation, and the BIS1 equation were compared.

Results

The median of difference between estimated GFR and standard GFR ranged from ?9.78 to 1.79 ml/min/1.73 m². The interquartile range of difference ranged from 12.38 to 20.87 ml/min/1.73 m². Accuracy with a deviation <30 % ranged from 44.9 to 66.3 %. However, none of the equations had accuracy up to the 70 % level. Bland–Altman analysis demonstrated that the precision ranged from 50.5–87.6 ml/min/1.73 m². The slopes of regression line ranged from ?0.26 to 0.55 and the intercepts ranged from ?18.71 to 11.07. In both the overall performance and the performances in different stage of CKD, GFR estimated by the BIS1 equation performed better results.

Conclusion

None of the GFR estimating equations are suitable for the specific Chinese population tested. At present, the BIS1 equation may be the optimal one for elderly Chinese CKD patients.     

Mesoaccumbens dopamine signaling alteration underlies behavioral transition from tolerance to sensitization to morphine rewarding properties during morphine withdrawal

Although the firing activity of dopamine (DA) neurons in the ventral tegmental area (VTA) and the behavioral response to morphine rewarding properties alter as opiate withdrawal, little is known about the dynamic changes in DA signal pathway from the VTA to the nucleus accumbens (NAc) during prolonged withdrawal, and whether the changes are indicative of vulnerability to relapse of drug abuse. Here we report that morphine spontaneously withdrawn (SW) rats are incapable of responding to small dose of morphine-induced conditioned place preference (CPP) from 24h-SW to 30d-SW, but recover response at 45d-SW. Interestingly, mesoaccumbens DA signaling, including the firing of DA neurons in the VTA, contents of DA and its metabolic ratio, and the membrane level of dopamine D₁ receptor in the NAc elicited by morphine challenge, display a similar pattern of time-dependent changes during morphine withdrawal. Moreover, blockade of D₁ receptor abolishes this behavioral transition. In addition, a strong correlation was found between % change in CPP score and membrane D₁ receptor level induced by morphine challenge. These results indicate a time-dependent behavioral switch from tolerance to sensitization during the prolonged withdrawal, which could offer a window for therapeutic intervention via manipulations of D₁ receptors.     

药膳食疗在临床营养治疗中的应用

中医学是中华民族文化的一大辉煌成就,是炎黄子孙几千年来不断实践、总结、完善、发展的科学结晶。中医药膳食疗就是这伟大成就中的一部分。随着经济的发展,人民生活水平的提高,工作节奏的加快,药膳食疗的保健治疗作用越来越被人们所重视和接受。     

多囊卵巢综合征患者餐后脂代谢紊乱相关因素的研究

目的:探讨PCOS患者餐后脂代谢紊乱主要影响因素,为临床治疗提供指导。方法:纳入符合鹿特丹标准PCOS患者68例,非PCOS患者68例,比较PCOS组与对照组餐后脂代谢指标甘油三酯(TG)、高密度脂蛋白(HDL-C)、载脂蛋白A(Apo A)、载脂蛋白B(Apo B)、性激素结合球蛋白(SHBG)的差异,并做相关性分析。结果 PCOS组与对照组餐后2h TG、AUC-TG、餐后4h血糖、AUC-血糖、胰岛素、AUC-胰岛素、i AUC-胰岛素有显著差异(P0.05);PCOS组0、2、4、6 h的HDL-C及AUC-HDL-C水平明显低于对照组(P0.05);两组间载脂蛋白无显著差异(P0.05)。结论:胰岛素抵抗是导致PCOS患者血脂异常和心血管疾病风险增加的重要因素,在治疗肥胖的PCOS患者时,应该优先改善患者的胰岛素抵抗。     

产后访视对母婴保健的影响

目的 及时了解产后访视和护理指导对母婴健康状况及母乳喂养的影响。方法 对512例产妇进行产后建库、建册，选派护师以上长期从事妇产科临床护理工作，有专业理论知识，又有临床护理经验的护士按社区工作规定的时间分别在产后3-7d，10～14d及28-30d进行访视。结果 及时了解了母婴健康状况，解决了病人出院后护理支持不足的问题，产妇及其家属给予了充分的肯定，满意度达100％。结论 产后访视和护理指导，有利于母婴的健康教育，使母婴健康得到保障，使母乳喂养的实施得到普及，取得良好的社会效益。