荧光原位杂交技术分析人结肠菌群方法研究

建立荧光原位杂交技术分析人体内结肠菌群的方法。取受试者新鲜粪便 ,选用 5种特异性的 16SrRNA寡核苷酸探针 ,检测粪便样本收集后的保存时间、温度 ,离心条件及样本固定液存放时间对杂交计数结果的影响。结果建立最佳实验条件为 :粪便样本收集后应尽快在 4℃下保存 ,放置时间不要超过 12小时即作处理 ;样本的适宜离心条件为 70 0g 2分钟 ;样本用多聚甲醛固定后在 - 80℃下存放时间不要超过 5个月。该方法具有较好的稳定性 ,可以有效地检出个体之间结肠菌群的差异。     

Balneoclimatic prevention and treatment of vascular disorders due to vibration at the forestry workers

Summary A total of 824 chain-saw operators who were employed in felling trees in the forestry industry were exposed to local vibration, which produced peripheral blood-flow disorders and lesions in the joints and hand bones. In the last 10 years, they have undergone 24-day balneoclimatic treatment at the Kolobrzeg Health Resort. It was found that a stay in the spa and certain balneological treatments exert a favorable effect on the peripheral blood-flow disorders produced by the local vibration of chain saws. The symptoms (chills and numbing of the fingers, etc.) disappeared in nearly 40% of these workers. An improvement in the objective indices of the peripheral circulation (thermographic and plethysmographic tests) was observed in about 90% of patients treated with brine baths combined with underwater vibratory massage (rotatory) or brine baths alone. The brine bath and vibratory massage have a direct beneficial influence on the peripheral blood flow of pectoral limbs.