全文获取类型
收费全文 | 424篇 |
免费 | 26篇 |
国内免费 | 1篇 |
专业分类
儿科学 | 1篇 |
妇产科学 | 5篇 |
基础医学 | 61篇 |
口腔科学 | 28篇 |
临床医学 | 32篇 |
内科学 | 86篇 |
皮肤病学 | 47篇 |
神经病学 | 26篇 |
特种医学 | 7篇 |
外科学 | 57篇 |
综合类 | 3篇 |
一般理论 | 1篇 |
预防医学 | 33篇 |
眼科学 | 19篇 |
药学 | 25篇 |
中国医学 | 3篇 |
肿瘤学 | 17篇 |
出版年
2023年 | 4篇 |
2022年 | 9篇 |
2021年 | 14篇 |
2020年 | 9篇 |
2019年 | 15篇 |
2018年 | 14篇 |
2017年 | 11篇 |
2016年 | 16篇 |
2015年 | 25篇 |
2014年 | 21篇 |
2013年 | 31篇 |
2012年 | 41篇 |
2011年 | 47篇 |
2010年 | 16篇 |
2009年 | 21篇 |
2008年 | 20篇 |
2007年 | 22篇 |
2006年 | 17篇 |
2005年 | 15篇 |
2004年 | 10篇 |
2003年 | 16篇 |
2002年 | 11篇 |
2001年 | 8篇 |
2000年 | 5篇 |
1999年 | 1篇 |
1998年 | 1篇 |
1997年 | 1篇 |
1996年 | 1篇 |
1995年 | 1篇 |
1994年 | 4篇 |
1993年 | 5篇 |
1992年 | 1篇 |
1991年 | 2篇 |
1990年 | 1篇 |
1989年 | 1篇 |
1987年 | 2篇 |
1984年 | 1篇 |
1983年 | 1篇 |
1982年 | 1篇 |
1981年 | 1篇 |
1980年 | 1篇 |
1977年 | 2篇 |
1975年 | 1篇 |
1974年 | 2篇 |
1964年 | 1篇 |
1941年 | 1篇 |
排序方式: 共有451条查询结果,搜索用时 62 毫秒
1.
Toidi Adekambi Chris C. Ibegbu Stephanie Cagle Ameeta S. Kalokhe Yun F. Wang Yijuan Hu Cheryl L. Day Susan M. Ray Jyothi Rengarajan 《The Journal of clinical investigation》2015,125(5):1827-1838
BACKGROUND. The identification and treatment of individuals with tuberculosis (TB) is a global public health priority. Accurate diagnosis of pulmonary active TB (ATB) disease remains challenging and relies on extensive medical evaluation and detection of Mycobacterium tuberculosis (Mtb) in the patient’s sputum. Further, the response to treatment is monitored by sputum culture conversion, which takes several weeks for results. Here, we sought to identify blood-based host biomarkers associated with ATB and hypothesized that immune activation markers on Mtb-specific CD4+ T cells would be associated with Mtb load in vivo and could thus provide a gauge of Mtb infection.METHODS. Using polychromatic flow cytometry, we evaluated the expression of immune activation markers on Mtb-specific CD4+ T cells from individuals with asymptomatic latent Mtb infection (LTBI) and ATB as well as from ATB patients undergoing anti-TB treatment.RESULTS. Frequencies of Mtb-specific IFN-γ+CD4+ T cells that expressed immune activation markers CD38 and HLA-DR as well as intracellular proliferation marker Ki-67 were substantially higher in subjects with ATB compared with those with LTBI. These markers accurately classified ATB and LTBI status, with cutoff values of 18%, 60%, and 5% for CD38+IFN-γ+, HLA-DR+IFN-γ+, and Ki-67+IFN-γ+, respectively, with 100% specificity and greater than 96% sensitivity. These markers also distinguished individuals with untreated ATB from those who had successfully completed anti-TB treatment and correlated with decreasing mycobacterial loads during treatment.CONCLUSION. We have identified host blood-based biomarkers on Mtb-specific CD4+ T cells that discriminate between ATB and LTBI and provide a set of tools for monitoring treatment response and cure.TRIAL REGISTRATION. Registration is not required for observational studies.FUNDING. This study was funded by Emory University, the NIH, and the Yerkes National Primate Center. 相似文献
2.
Sebastiana Z. Kalula G. H. Swingler A. A. Sayer M. Badri M. Ferreira 《The journal of nutrition, health & aging》2010,14(4):319-323
Objective
To test the effects of the use of a collapsible, portable chair (chair B), as opposed to a ‘standard’ chair (chair A), on the outcome of the timed “Up and Go” (TUG) test. 相似文献3.
4.
5.
The objective of this study is to develop a topical bead formulation of tranexamic acid (TA) which can be used concomitantly with laser treatment. The bead formulation of TA (TAB) was successfully prepared by fluidized bed drying method. Physicochemical properties of the TAB were evaluated in terms of chemical stability of TA and differential scanning calorimetry. TA in the bead was stable up to six months at 25°C and existed as amorphous state. In vitro skin permeation and in vivo skin retention of TA in the beads were significantly higher compared to a commercial product. When the bead was dissolved into distilled water and applied concomitantly with laser treatment, the amount of TA retained in the skin in the in vivo study was inversely proportional to the energy levels of laser treatment, indicating absorption into subcutaneous tissue and drainage to systemic circulation. Therefore, when laser treatment is used concomitantly with TAB, energy level should be very carefully monitored to avoid possible adverse events associated with systemic side effects of TA. 相似文献
6.
7.
8.
9.
Joo-Yong Jung Ranjna Madan-Lala Maria Georgieva Jyothi Rengarajan Charles D. Sohaskey Franz-Christoph Bange Cory M. Robinson 《Infection and immunity》2013,81(9):3198-3209
Nitric oxide (NO) is a diffusible radical gas produced from the activity of nitric oxide synthase (NOS). NOS activity in murine macrophages has a protective role against mycobacteria through generation of reactive nitrogen intermediates (RNIs). However, the production of NO by human macrophages has remained unclear due to the lack of sensitive reagents to detect NO directly. The purpose of this study was to investigate NO production and the consequence to mycobacteria in primary human macrophages. We found that Mycobacterium bovis BCG or Mycobacterium tuberculosis infection of human macrophages induced expression of NOS2 and NOS3 that resulted in detectable production of NO. Treatment with gamma interferon (IFN-γ), l-arginine, and tetrahydrobiopterin enhanced expression of NOS2 and NOS3 isoforms, as well as NO production. Both of these enzymes were shown to contribute to NO production. The maximal level of NO produced by human macrophages was not bactericidal or bacteriostatic to M. tuberculosis or BCG. The number of viable mycobacteria was increased in macrophages that produced NO, and this requires expression of nitrate reductase. An narG mutant of M. tuberculosis persisted but was unable to grow in human macrophages. Taken together, these data (i) enhance our understanding of primary human macrophage potential to produce NO, (ii) demonstrate that the level of RNIs produced in response to IFN-γ in vitro is not sufficient to limit intracellular mycobacterial growth, and (iii) suggest that mycobacteria may use RNIs to enhance their survival in human macrophages. 相似文献
10.
Rengarajan Rajagopal Nitin Mishra Nitin Yadav Vikas Jhanwar Ajit Thakur Naima Mannan 《African Journal of Paediatric Surgery》2015,12(2):140-142