全文获取类型
收费全文 | 1673篇 |
免费 | 98篇 |
国内免费 | 15篇 |
专业分类
耳鼻咽喉 | 18篇 |
儿科学 | 81篇 |
妇产科学 | 40篇 |
基础医学 | 164篇 |
口腔科学 | 39篇 |
临床医学 | 118篇 |
内科学 | 429篇 |
皮肤病学 | 20篇 |
神经病学 | 141篇 |
特种医学 | 194篇 |
外科学 | 209篇 |
综合类 | 53篇 |
一般理论 | 2篇 |
预防医学 | 35篇 |
眼科学 | 49篇 |
药学 | 119篇 |
中国医学 | 6篇 |
肿瘤学 | 69篇 |
出版年
2024年 | 2篇 |
2023年 | 38篇 |
2022年 | 34篇 |
2021年 | 78篇 |
2020年 | 50篇 |
2019年 | 61篇 |
2018年 | 70篇 |
2017年 | 29篇 |
2016年 | 50篇 |
2015年 | 81篇 |
2014年 | 58篇 |
2013年 | 86篇 |
2012年 | 122篇 |
2011年 | 82篇 |
2010年 | 93篇 |
2009年 | 88篇 |
2008年 | 96篇 |
2007年 | 74篇 |
2006年 | 58篇 |
2005年 | 43篇 |
2004年 | 42篇 |
2003年 | 43篇 |
2002年 | 43篇 |
2001年 | 16篇 |
2000年 | 11篇 |
1999年 | 12篇 |
1998年 | 24篇 |
1997年 | 43篇 |
1996年 | 33篇 |
1995年 | 29篇 |
1994年 | 23篇 |
1993年 | 19篇 |
1992年 | 5篇 |
1991年 | 8篇 |
1990年 | 9篇 |
1989年 | 22篇 |
1988年 | 17篇 |
1987年 | 10篇 |
1986年 | 12篇 |
1985年 | 7篇 |
1984年 | 8篇 |
1983年 | 11篇 |
1982年 | 10篇 |
1981年 | 7篇 |
1980年 | 7篇 |
1978年 | 3篇 |
1977年 | 4篇 |
1976年 | 6篇 |
1972年 | 2篇 |
1948年 | 1篇 |
排序方式: 共有1786条查询结果,搜索用时 15 毫秒
1.
Ashwin A Kallianpur Rajinder Parshad Maya Dehran Priya Hazrah 《JSLS, Journal of the Society of Laparoendoscopic Surgeons》2007,11(2):229-234
BACKGROUND AND OBJECTIVES: Feasibility of ambulatory laparoscopic inguinal hernia repair in developing countries is not known due to lack of dedicated outpatient centers. This study prospectively evaluated the feasibility of outpatient discharge after laparoscopic total extraperitoneal inguinal hernia repair done in combination with in-hospital services and its impact on quality of life. METHODS: Forty patients were studied who had uncomplicated inguinal hernias and fulfilled the selection criteria. Quality of life was evaluated by using the SF-12 questionnaire. RESULTS: Ninety percent of patients could be discharged as outpatients. Four patients required admission. No major complications or readmissions occurred. Physical components of quality of life deteriorated in the immediate postoperative period but improved to above preoperative levels within one month. A transient deterioration in subgroups of the mental health component was observed, which recovered to normal in less than a week. There was no significant alteration in the emotional component. There has been no recurrence at a median follow-up of 25 months. CONCLUSION: It was feasible to safely perform outpatient TEP in combination with routine in-hospital services without increasing complications or causing any adverse impact on quality of life. This was possible subject to adherence to proper selection and discharge criteria. 相似文献
2.
3.
Hausegger KA; Cragg AH; Lammer J; Lafer M; Fluckiger F; Klein GE; Sternthal MH; Pilger E 《Radiology》1994,190(1):199
4.
5.
6.
7.
8.
9.
10.
CP Schaecher KA Groesch 《American journal of reproductive immunology (New York, N.Y. : 1989)》2006,55(6):405-405
Background: Control of mRNA stability is an essential regulatory process in eukaryotic gene expression. HuR, a 3'UTR mRNA binding protein, can protect AU-rich mRNA from degradation in response to stresses. PlGF, an angiogenic growth factor, contains two consensus AU-rich sites suggesting that under normal conditions HuR may protect PlGF mRNA from degradation. Trophoblast expression of PlGF is significantly decreased in preeclampsia and by hypoxia in vitro . We hypothesize that decreased levels of cytoplasmic HuR may contribute to decreased PlGF expression in hypoxic and preeclamptic trophoblast.
Methods: Western blots were used to determine relative effects of in vitro hypoxia on HuR protein expression and subcellular localization in trophoblast. Immunohistochemistry was used to compare HuR expression patterns in trophoblast of preeclamptic and normal placentae.
Results: Cytoplasmic expression of HuR was decreased 1.4 fold in the cytoplasm and 1.2 fold in the nucleus of JEG3 cells. A shift in HuR was more apparent in primary trophoblast with a greater than 2-fold decrease in the cytoplasm and a 1.4 fold decrease in the nucleus following 24 hr of hypoxia. Immunohistochemical analyses detected HuR expression in near term trophoblast in situ . However, this technical approach did not detect a significant change in HuR expression between normal and preeclamptic trophoblast.
Conclusions: HuR expression is decreased in hypoxic trophoblast, at least in vitro , which may provide a causal link to decreased PlGF mRNA expression. Down regulation of trophoblast PlGF expression is thought to contribute to the pathophysiology associated with preeclampsia including the relative lack of perfusion of the placenta and systemic renal effects. 相似文献
Methods: Western blots were used to determine relative effects of in vitro hypoxia on HuR protein expression and subcellular localization in trophoblast. Immunohistochemistry was used to compare HuR expression patterns in trophoblast of preeclamptic and normal placentae.
Results: Cytoplasmic expression of HuR was decreased 1.4 fold in the cytoplasm and 1.2 fold in the nucleus of JEG3 cells. A shift in HuR was more apparent in primary trophoblast with a greater than 2-fold decrease in the cytoplasm and a 1.4 fold decrease in the nucleus following 24 hr of hypoxia. Immunohistochemical analyses detected HuR expression in near term trophoblast in situ . However, this technical approach did not detect a significant change in HuR expression between normal and preeclamptic trophoblast.
Conclusions: HuR expression is decreased in hypoxic trophoblast, at least in vitro , which may provide a causal link to decreased PlGF mRNA expression. Down regulation of trophoblast PlGF expression is thought to contribute to the pathophysiology associated with preeclampsia including the relative lack of perfusion of the placenta and systemic renal effects. 相似文献