全文获取类型
收费全文 | 934篇 |
免费 | 51篇 |
国内免费 | 5篇 |
专业分类
耳鼻咽喉 | 2篇 |
儿科学 | 37篇 |
妇产科学 | 4篇 |
基础医学 | 146篇 |
口腔科学 | 8篇 |
临床医学 | 64篇 |
内科学 | 209篇 |
皮肤病学 | 48篇 |
神经病学 | 71篇 |
特种医学 | 23篇 |
外科学 | 90篇 |
综合类 | 4篇 |
预防医学 | 19篇 |
眼科学 | 49篇 |
药学 | 70篇 |
中国医学 | 1篇 |
肿瘤学 | 145篇 |
出版年
2023年 | 9篇 |
2022年 | 17篇 |
2021年 | 26篇 |
2020年 | 22篇 |
2019年 | 22篇 |
2018年 | 24篇 |
2017年 | 27篇 |
2016年 | 22篇 |
2015年 | 29篇 |
2014年 | 22篇 |
2013年 | 37篇 |
2012年 | 37篇 |
2011年 | 44篇 |
2010年 | 25篇 |
2009年 | 23篇 |
2008年 | 35篇 |
2007年 | 34篇 |
2006年 | 38篇 |
2005年 | 33篇 |
2004年 | 34篇 |
2003年 | 46篇 |
2002年 | 45篇 |
2001年 | 37篇 |
2000年 | 45篇 |
1999年 | 23篇 |
1998年 | 13篇 |
1997年 | 8篇 |
1996年 | 5篇 |
1995年 | 8篇 |
1994年 | 3篇 |
1993年 | 4篇 |
1992年 | 15篇 |
1991年 | 17篇 |
1990年 | 9篇 |
1989年 | 10篇 |
1988年 | 18篇 |
1987年 | 17篇 |
1986年 | 15篇 |
1985年 | 20篇 |
1984年 | 4篇 |
1983年 | 9篇 |
1981年 | 7篇 |
1979年 | 13篇 |
1978年 | 6篇 |
1977年 | 7篇 |
1975年 | 3篇 |
1971年 | 2篇 |
1970年 | 3篇 |
1969年 | 3篇 |
1966年 | 6篇 |
排序方式: 共有990条查询结果,搜索用时 15 毫秒
1.
Satoshi Iyama Yasuo Takahashi Naoaki Shintani Koshi Fujikawa Syunichi Ohkubo Yasushi Sato Tsutomu Sato Yasuhiro Sato Keisuke Ohnuma Yoshiro Niitsu 《Nihon Shokakibyo Gakkai zasshi》2006,103(10):1163-1168
We report a case of biliary cystadenocarcinoma which recurred 41 months postoperatively. A 60-year-old woman was admitted for further examination of multiple metastatic tumors and a large amount of ascites. Systemic administration of 5FU and CDDP caused her CEA level to decrease gradually and abdominal computed tomography revealed considerable reduction of the metastatic tumors and ascites. Since her general condition had improved, chemotherapy was continued in the outpatient clinic. 相似文献
2.
Effects of herbal medicine Dai-Kenchu-to on anorectal function in children with severe constipation.
AIM: We administered the herbal medicine Dai-Kenchu-To (DKT) to children with severe chronic constipation or with severe constipation after surgery for anorectal malformations. We then objectively assessed the effect of DKT on anorectal function by manometric study in addition to using a clinical scoring system. PATIENTS AND METHODS: Ten children with severe chronic constipation and 5 children with severe constipation after surgery for anorectal malformations were assessed. These 15 children received 0.3 g/kg/day of DKT for periods ranging from 3 months to 1 year. We objectively assessed their bowel function, sphincter function and rectal reservoir function by anorectal manometry and clinical scoring. RESULTS: In 10 children with severe chronic constipation, the clinical score after administration of DKT (7.2 +/- 0.8) improved significantly compared with that before administration of DKT (4.6 +/- 2.9) (p < 0.02). The threshold sensation volume and the maximum tolerable volume after administration of DKT significantly (p < 0.05; p < 0.01) decreased (128 +/- 63 ml vs. 69 +/- 18 ml; 229 +/- 99 ml vs. 144 +/- 47 ml), and rectal compliance after administration of DKT also significantly (p < 0.05) decreased (12.4 +/- 10.9 ml/cmH(2)O vs. 4.7 +/- 3.9 ml/cmH(2)O). CONCLUSION: The present study demonstrated that DKT had a favorable clinical effect on severe constipation in children, and anorectal manometry showed an improvement in their rectal reservoir functions. It appears that the results were secondary to DKT-stimulated peristalsis of the intestine, which promoted regular bowel habits. 相似文献
3.
Mutational screening of APP gene in patients with early-onset Alzheimer disease utilizing mismatched PCR-RFLP 总被引:1,自引:0,他引:1
Yumiko Nishiwaki Kouzin Kamino Aoi Yoshiiwa Keiko Nagano Masatoshi Takeda Hirotaka Tanabe Tsuyoshi Nishimura Toshiko Kobayashi Hideki Yamamoto Yasuhiro Nonomura Hiroshi Yoneda Toshiaki Sakai Masaki Imagawa Tetsuro Miki Toshio Ogihara 《Clinical genetics》1996,49(3):119-123
To elucidate the frequency of mutations of the β/A4 amyloid protein precursor (APP) gene in early-onset Alzheimer disease, we designed a mismatched PCR-RFLP that can identify all kinds of missense mutations at codon 717 in addition to the seven kinds of known mutations at exon 17. When we screened mutations at exon 17 utilizing this method and the double missense mutations at exon 16 of the APP gene by PCR-RFLP, no cases revealed mutations of the APP gene among 13 familial and 54 sporadic cases, except one family (OS-1) that had previously been reported and used as a positive control of APP717(Val → Ile). Our results support the hypothesis that mutations in the APP gene are not major causes in early-onset Alzheimer disease. 相似文献
4.
Aoi W Naito Y Sakuma K Kuchide M Tokuda H Maoka T Toyokuni S Oka S Yasuhara M Yoshikawa T 《Antioxidants & redox signaling》2003,5(1):139-144
Dietary antioxidants may attenuate oxidative damage from strenuous exercise in various tissues. Beneficial effects of the antioxidant astaxanthin have been demonstrated in vitro, but not yet in vivo. We investigated the effect of dietary supplementation with astaxanthin on oxidative damage induced by strenuous exercise in mouse gastrocnemius and heart. C57BL/6 mice (7 weeks old) were divided into groups: rested control, intense exercise, and exercise with astaxanthin supplementation. After 3 weeks of exercise acclimation, both exercise groups ran on a treadmill at 28 m/min until exhaustion. Exercise-increased 4-hydroxy-2-nonenal-modified protein and 8-hydroxy-2'-deoxyguanosine in gastrocnemius and heart were blunted in the astaxanthin group. Increases in plasma creatine kinase activity, and in myeloperoxidase activity in gastrocnemius and heart, also were lessened by astaxanthin. Astaxanthin showed accumulation in gastrocnemius and heart from the 3 week supplementation. Astaxanthin can attenuate exercise-induced damage in mouse skeletal muscle and heart, including an associated neutrophil infiltration that induces further damage. 相似文献
5.
6.
Use of cultured tubular cells isolated from human urine for investigation of renal transporter 总被引:2,自引:0,他引:2
AIM: To facilitate the understanding of the transporter function of human renal tubular cells, we have developed a simple method using primary cultured proximal tubule (PT) cells isolated from voided urine. METHODS: PT cells grown to confluence on glass coverslips could be identified by parallel arrays of spindle cells and hemicyst formation. Brush-border gamma-glutamyl transpeptidase (gammaGTP) activity was histochemically identified. Apical membrane Na+/H+ exchanger (NHE) activity was measured by monitoring changes in intracellular pH (pHi) after an acid load in a single cell level using the pH-sensitive dye 2'7'-bis-(2-carboxyethyl)-5.6'carboxyfluorescein (BCECF). RESULTS: Amiloride and 5-(N-ethyl-N-isopropyl) amiloride (EIPA) inhibited the NHE activity with half-maximal inhibition values (IC50) of 15.3 and 4.0 microM, respectively. NHE-3 mRNA was detected by the RT-PCR technique in clonally proliferated PT cells. CONCLUSION: These results suggest that cultured PT cells isolated from human urine express amiloride-resistant NHE-3 activity on the apical membranes, which can be compared to functional properties of PT in vivo. Our experimental strategy offers a useful experimental approach to investigating human renal tubular transport function in vitro. 相似文献
7.
Glial cell line-derived neurotrophic factor (GDNF) is a member of the transforming growth factor beta superfamily and acts as a neurotrophic factor for the nigrostriatal dopaminergic system. GDNF at a dose of 100 micrograms was injected stereotactically into the striatum of Sprague-Dawley rats that had been treated with intrastriatal 6-hydroxydopamine (6-OHDA) injection four weeks earlier. Immunocytochemical and behavioral analyses showed significant recovery of the nigrostriatal dopaminergic system after a single GDNF injection or continuous GDNF injection. Immunocytochemical and behavioral study showed that there was no significant difference between the results obtained from the two different injection methods. This result demonstrates the potential usefulness of GDNF for the treatment of Parkinson's disease. 相似文献
8.
Ishitobi M Haginoya K Zhao Y Ohnuma A Minato J Yanagisawa T Tanabu M Kikuchi M Iinuma K 《Neuroreport》2000,11(18):4033-4035
The progression of muscular weakness of patients suffering from muscular dystrophies directly correlates with the progressive loss of myofibers, accompanied by fibrosis. Since transforming growth factor beta1 (TGF-beta1) promotes tissue fibrosis, we measured the plasma TGF-beta1 level in patients with various muscular dystrophies in order to determine whether the level is elevated in patients with muscular dystrophy and if the level reflects the severity of tissue fibrosis. The plasma TGF-beta1 level was significantly elevated in patients with Duchenne muscular dystrophy and congenital muscular dystrophy (CMD), but not in those with Becker muscular dystrophy. Growth factors related to muscle fiber regeneration and fibrosis might be a key factor in the progression of muscular dystrophy and could be a target for therapeutic studies. 相似文献
9.
10.
Dihydropyrimidine dehydrogenase activity in 150 healthy Japanese volunteers and identification of novel mutations. 总被引:4,自引:0,他引:4