CXCL1在膀胱移行细胞癌中的表达

目的研究CXCL1在膀胱移行细胞癌组织中的表达及其与膀胱癌侵袭、转移的关系。方法应用链霉菌抗生物素蛋白一过氧化酶（sP）免疫组织化学方法,检测5例正常膀胱组织和49例膀胱移行细胞癌组织中CXCL1的表达水平。结果CXCL1在膀胱正常组织中均呈阴性表达,在49例膀胱移行细胞癌组织中均不同程度的表达,随肿瘤分级的增高,CXC1的表达显著增强（P〈O,05）;随肿瘤分期的增高,CXCL1的表达也显著增强（P〈0．05.结论CXCL1与臃胱癌的侵袭转夥有美．有可能成为阻止肿瘪侵袭治疗的一个靶点。     

HuR与环氧化酶-2在卵巢上皮性癌表达及相关性研究

Objective To detect the expressions of HuR and COX-2 in epithelial ovarian carcinoma,and investigate the correlation of HuR and COX-2 expression. In addition, we attempt to seek the pathway to prevent the occurrence and development of epithelial ovarian cancer by combined analysis of various clinicopathologic characteristics. Methods The expressions of HuR and COX-2 in 68 epithelial ovarian carcinoma, 10 borderline ovarian tumors and 5 normal ovarian tissues were examined by S-P immunohistochemical method. The relationship of HuR and COX-2 expressions with clinicopathologic parameterwere evaluated by correlation analysis. Results(1) The expression of HuR in epithelial ovarian cancer tissue (76. 47% ,52/68) was significantly higher than that in borderline epithelial ovarian tumor tissues (30. 00% ,3/10) and normal ovarian tissues (0, x2 = 18. 873, Ps ＜ 0. 05), but there were no significant differences betweenthe expressions of HuR in borderline epithelial ovarian tumor and normal ovarian tissue(P ＞ 0. 05).(2) The positive expression rate of cytoplasmic HuR in epithelial ovarian carcinoma and borderline epithelial ovarian tumor were 45.60% (31/68) and 10. 00% (1/10) respectively,but normal ovarian tissues showed no staining of HuR. We found no significant differences between the expression of cytoplasmic HuR in epithelial ovarian carcinoma and borderline epithelial ovarian tumor or normal ovarian tissue(x2 = 7. 999 ,P =0. 018).(3) The positive expression rate of cytoplasmic HuR in epithelial ovarian carcinoma of FIGO stage Ⅲ - Ⅳ was significantly higher than that of stage Ⅰ - Ⅱ(56. 09% vs. 29. 63%, x2 = 4. 598, P = 0. 032). The positive expression rates of cytoplasmic HuR in epithelial ovarian carcinoma of histological grade 1,2,3 were 10. 00%,46. 67% ,57. 14% respectively, which showed significant difference in the comparison among the three groups (x2 =6. 627 ,P =0. 036). (4) The positive expression rates of COX-2 in epithelial ovarian cancer (67. 64%)and borderline epithelial ovarian tumor tissues (60. 00%) were significantly higher than that in normal ovarian tissues (0, Ps ＜ 0. 05), but we found no significant difference in the comparison between the expression of malignant and borderline ovarian tumors. Statistical analysis showed that the positive expression rate of COX-2 in epithelial ovarian carcinoma was correlated with FIGO stage and lymph node metastasis. (5)There was a significantly positive correlation between cytoplasmic HuR and COX-2 expressions in epithelial ovarian carcinoma. Conclusion The expressions of HuR and COX-2 increased in the epithelial ovarian carcinoma, and the cytoplasmic expression of HuR was significantly correlated with the expression of COX-2. These results suggested that increased cytoplasmic expression of HuR and COX-2 expression might play important roles in the initiation and development of epithelial ovarian carcinoma.     

毛萼乙素通过调节STAT3通路抑制宫颈癌细胞增殖与转移的研究

目的 探究毛萼乙素通过调节STAT3通路抑制宫颈癌细胞增殖与转移的效果。方法 5、10、20μmol/L毛萼乙素处理宫颈癌细胞系SiHa 48 h,通过CCK8检测细胞增殖,通过流式细胞术检测细胞周期分布,通过Transwell实验检测细胞迁移与侵袭能力,通过Western blot检测CDK2、Cyclin E、E-caderin、MMP9、p-JAK2、JAK2、p-STAT3、STAT3表达。使用毛萼乙素（20μmol/L）单独或联合STAT3激动剂Colivelin(1μg/mL)处理SiHa细胞48 h,通过CCK8检测细胞增殖,通过Transwell实验检测细胞迁移与侵袭能力。结果 EriB以剂量依赖性的方式抑制细胞增殖速率,SiHa细胞系细胞周期出现明显停滞,同时EriB还能进一步抑制SiHa细胞系的迁移及侵袭能力;EriB处理后SiHa细胞系中CDK2、Cyclin E、E-caderin、MMP9、p-JAK2、p-STAT3表达下降（P<0.05）。联合给药STAT3激动剂Colivelin可抵消EriB对于SiHa细胞增殖、迁移与侵袭的影响（P<0.0...     

诸城市1992～2001年新发涂阳肺结核病例分析

1992年11月～2001年,诸城市实施了世界银行贷款结核病控制项目(以下简称项目).为了解新发涂阳肺结核病人发病特征和疫情状况,更有效地控制传染源.现将10年来登记的新发涂阳肺结核病例进行分析.     

HuR与环氧化酶-2在卵巢上皮性癌表达及相关性研究

目的 探讨HuR和环氧化酶(COX-2)与卵巢上皮性癌临床病理参数的关系及二者的相关性.方法 收集原发卵巢上皮性癌组织68例(卵巢上皮性癌组),交界性卵巢肿瘤10例(交界性卵巢肿瘤组),正常卵巢组织5例(正常卵巢组织组)行免疫组织化学染色SP法检测HuR与COX-2的表达及与临床病理参数的关系.结果 (1)HuR在卵巢上皮性癌胞核中阳性表达率为76.47%(52/68)明显高于交界性卵巢肿瘤胞核30.00%(3/10)和正常卵巢组织(正常卵巢组织无表达)(x2=18.873,P＜0.05);HuR在交界性卵巢肿瘤的表达与正常卵巢组织比较差异无统计学意义(P＞0.05).(2)HuR在卵巢上皮性癌和交界性卵巢肿瘤胞质中的阳性表达率分别为45.60%(31/68)、10.00%(1/10),正常卵巢组织无表达;胞质HuR在恶性卵巢肿瘤组织的表达与交界性卵巢肿瘤和正常卵巢组织比较差异有统计学意义(x2=7.999,P=0.018);胞质HuR在交界性卵巢肿瘤的表达与正常卵巢组织比较差异无统计学意义(P＞0.05).(3)临床分期Ⅲ、Ⅳ期胞质HuR的阳性表达率为56.09%(23/41),显著高于Ⅰ、Ⅱ期的29.63%(8/27),差异有统计学意义(x2=4.598,P=0.032).在组织学分级高分化、中分化、低分化胞质HuR的阳性表达率分别为10.00%(1/10)、46.67%(14/30)、57.14%(16/28),差异有统计学意义(x2=6.627,P=0.036).(4)COX-2在卵巢上皮性癌(67.64%)和交界性卵巢肿瘤(60.00%)的表达明显高于正常卵巢组织(0),在卵巢上皮性癌的表达与交界性卵巢肿瘤比较差异无统计学意义(P＞0.05);COX-2高表达与临床分期和淋巴结转移有关;(5)胞质HuR与COX-2在肿瘤中的表达呈正相关(x2=0.317,P=0.008).结论 HuR与COX-2在卵巢上皮性癌的表达明显增高,且胞质HuR的表达与COX-2的表达呈正相关,提示胞质HuR与COX-2的过表达可能与卵巢癌的发生发展密切相关.

Abstract：

Objective To detect the expressions of HuR and COX-2 in epithelial ovarian carcinoma,and investigate the correlation of HuR and COX-2 expression. In addition, we attempt to seek the pathway to prevent the occurrence and development of epithelial ovarian cancer by combined analysis of various clinicopathologic characteristics. Methods The expressions of HuR and COX-2 in 68 epithelial ovarian carcinoma, 10 borderline ovarian tumors and 5 normal ovarian tissues were examined by S-P immunohistochemical method. The relationship of HuR and COX-2 expressions with clinicopathologic parameterwere evaluated by correlation analysis. Results(1) The expression of HuR in epithelial ovarian cancer tissue (76. 47% ,52/68) was significantly higher than that in borderline epithelial ovarian tumor tissues (30. 00% ,3/10) and normal ovarian tissues (0, x2 = 18. 873, Ps ＜ 0. 05), but there were no significant differences betweenthe expressions of HuR in borderline epithelial ovarian tumor and normal ovarian tissue(P ＞ 0. 05).(2) The positive expression rate of cytoplasmic HuR in epithelial ovarian carcinoma and borderline epithelial ovarian tumor were 45.60% (31/68) and 10. 00% (1/10) respectively,but normal ovarian tissues showed no staining of HuR. We found no significant differences between the expression of cytoplasmic HuR in epithelial ovarian carcinoma and borderline epithelial ovarian tumor or normal ovarian tissue(x2 = 7. 999 ,P =0. 018).(3) The positive expression rate of cytoplasmic HuR in epithelial ovarian carcinoma of FIGO stage Ⅲ - Ⅳ was significantly higher than that of stage Ⅰ - Ⅱ(56. 09% vs. 29. 63%, x2 = 4. 598, P = 0. 032). The positive expression rates of cytoplasmic HuR in epithelial ovarian carcinoma of histological grade 1,2,3 were 10. 00%,46. 67% ,57. 14% respectively, which showed significant difference in the comparison among the three groups (x2 =6. 627 ,P =0. 036). (4) The positive expression rates of COX-2 in epithelial ovarian cancer (67. 64%)and borderline epithelial ovarian tumor tissues (60. 00%) were significantly higher than that in normal ovarian tissues (0, Ps ＜ 0. 05), but we found no significant difference in the comparison between the expression of malignant and borderline ovarian tumors. Statistical analysis showed that the positive expression rate of COX-2 in epithelial ovarian carcinoma was correlated with FIGO stage and lymph node metastasis. (5)There was a significantly positive correlation between cytoplasmic HuR and COX-2 expressions in epithelial ovarian carcinoma. Conclusion The expressions of HuR and COX-2 increased in the epithelial ovarian carcinoma, and the cytoplasmic expression of HuR was significantly correlated with the expression of COX-2. These results suggested that increased cytoplasmic expression of HuR and COX-2 expression might play important roles in the initiation and development of epithelial ovarian carcinoma.     

宫颈脱落细胞中miR-424和CUL2 mRNA表达对宫颈HPV16持续感染的预测价值

目的 研究宫颈脱落细胞中微小RNA-424(miR-424)和枯灵素2(CUL2)信使RNA(mRNA)表达水平对宫颈人乳头瘤病毒(HPV)16持续感染的预测价值。方法 选取2020年1月至2021年11月在邯郸市中心医院宫颈液基细胞学检查正常的259例宫颈HPV16感染者为研究对象,随访1年后复查,HPV16仍是阳性者归为HPV16持续感染组(136例),HPV16转为阴性者归为HPV16非持续感染组(123例)。对宫颈HPV16持续感染者在复查当日以组织病理学诊断结果进行分组：52例慢性宫颈炎症患者(宫颈炎症组),61例宫颈上皮内瘤变者(上皮瘤变组),23例宫颈鳞癌患者(宫颈鳞癌组)。采用荧光定量PCR法检测宫颈脱落细胞中miR-424和CUL2 mRNA表达水平,Pearson法分析HPV16持续感染者宫颈脱落细胞中miR-424和CUL2 mRNA的相关性,受试者工作特征曲线评价宫颈脱落细胞中miR-424和CUL2 mRNA表达水平对宫颈HPV16持续感染的预测价值。结果 与HPV16非持续感染组相比,HPV16持续感染组宫颈脱落细胞中miR-424表达水平较低,CUL2 m...     

胚胎致死异常视觉蛋白A与卵巢上皮性癌

卵巢癌是常见的妇科恶性肿瘤，其死亡率在妇科恶性肿瘤占居首位，因70％的患者就诊时已属晚期，其预后极差。因此，卵巢癌的早期诊断和治疗尤为重要。胚胎致死异常视觉蛋白A（HuR）是一种RNA结合蛋白，能与腺嘌呤，尿嘧啶富集元件（ARE）相结合，ARE参与细胞的生长、分化及免疫应答。HuR在恶性肿瘤中高表达，其胞质HuR的表达与肿瘤的临床期别及患者的预后直接相关。众多研究提示，环氧合酶2（COX－2）可能成为一项独立的判断卵巢癌预后的分子及生化指标，而HuR的表达与COX－2蛋白表达增加呈正相关。     

某中学毕业班学生结核病爆发流行调查分析

2003年5月,诸城实验中学高三·七班,发生1起结核病爆发流行,报告如下。1对象与方法1.1对象高三·七班学生共40人,年龄17～19岁,平均为17.2岁,其中男生25人,女生15人。学生来自农村和城市分别是32人和8人。36人接种过卡介苗,入校时体检及X线检查均无异常。2003年5月21日1名男同学因咳嗽、发热到综合医院就诊,被“发热门诊”隔离观察,后诊断为“肺结核”转到市防疫站结防科诊治,引起校方重视,要求对全班同学进行普查,并实施预防控制措施。1.2调查方法40名学生全部使用北京产纯蛋白衍生物(PPD)标准精制结核菌素5IU,进行左前臂皮内注射试验,7…     

GPC-3在卵巢透明细胞癌和浆液性癌中的差异表达

目的检测GPC-3在卵巢透明细胞癌与浆液性癌中的差异表达,判断卵巢透明细胞癌与不良预后的关系。方法采用免疫组化链霉菌抗生物素蛋白-过氧化酶连接(SP)法和逆转录RT-PCR技术,检测GPC-3在卵巢透明细胞癌和浆液性囊腺癌中的差异表达。结果 GPC-3在卵巢透明细胞癌中的表达明显高于卵巢浆液性囊腺癌(P0.05);且GPC-3在卵巢透明细胞癌中的表达与铂类耐药有关。结论 GPC-3的过表达与肿瘤的低分化和以铂类为基础的化疗耐药有关,而且与卵巢透明细胞癌的不良预后有关。