一株采自新疆虫草体内的真菌的分离鉴定及其抑菌作用研究

目的研究从新疆虫草(Ophiocordyceps gracilis(Grev.)G.H.Sung,J.M.Sung,Hywel-Jones&Spatafora)体内分离得到一株真菌SFYC003。方法经生理生化特征、形态学和ITS序列对比分离鉴定菌株,采用滤纸片法研究了该菌株的菌体及其发酵液对6株供试菌的抑制作用。结果所得菌株属于青霉属真菌变灰青霉(Penicillium canescens)的一个变种,命名为SFYC003,其对大肠埃希菌和金黄色葡萄球菌供试菌的抑菌活性较强。结论菌株SFYC003具有进一步研究及开发利用的潜在价值。     

一株分离自新疆细虫草菌核的弯颈霉鉴定及抑菌研究

目的：开发利用新疆细虫草资源,为新疆细虫草相关真菌的研究和开发利用提供基础资料。方法：研究从新疆细虫草菌核中分离得到一种白色菌株,编号为SFYT002,采用形态学观察和ITS以及18SrDNA测序,在GenBank比对,进行系统进化树分析,并采用滤纸片法研究其体外抗菌作用。结果：菌株SFYT002主要的特征为菌落白色棉绒状,瓶梗长在分枝的分生孢子梗上,瓶梗基部膨大,顶部急剧变细,部分颈部弯曲,孢子小且透亮,孢子有出芽现象,常形成聚集孢子头,分生孢子球形,亚球形至椭圆形。ITS和18SrDNA序列分析表明,弯颈霉属为单系发生,菌株SFYT002与膨大弯颈霉聚为一支。研究其抑菌作用表明,菌株SFYT002发酵液及其不同极性的提取物对4种常见致病菌,均有很强的抑制作用。结论：通过形态学和分子生物学特征将菌株SFYT002鉴定为膨大弯颈霉,具有较强的抑菌活性。     

The protective effect of cuminaldehyde on gastric mucosa in Rattus norregicus of experimental gastric ulcer北大核心CSCD

Aim To explore the effect of cuminaldehyde in cumin fruit on gastric ulcer and the protective mechanism via establishing the gastric ulcer model of rats was by ethanol injury. Methods Thirty-six male R. norregicus were divided into six groups: control group, model group, omeprazole positive control group and cuminaldehyde low, medium and high dosage groups. After seven days of continuous intragastric administration, the acute gastric ulcer of R. norregicus was tested by absolute alcohol. Gastric ulcer area, inhibition rate, gastric tissue antioxidant activity, serum inflammatory factors and gastric mucosal protective factors were detected in different groups. Results The results showed that cuminaldehyde significantly reduced the area of gastric ulcer and increased the inhibition rate of gastric ulcer. The inhibition rate of cuminaldehyde at high dose group was up to 74.65%, the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH) in gastric tissue significantly increased, and the contents of serum prolandin E2(PGE2) and NO in gastric tissue also significantly increased. The content of malondialdehyde (MDA) decreased. Compared with the model group, cuminaldehyde decreased the content of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in rat serum, and decreased the activity of myeloperoxidase (MPO). Conclusions Cuminaldehyde has good antioxidant stress ability and anti-inflammatory activity, increases the expression of protective factors, enhances the defense ability of gastric mucosa, and has obvious protective effect on acute gastric ulcer in R. norregicus. © 2023 Publication Centre of Anhui Medical University. All rights reserved.