环孢菌素抑制钙离子介导的HL-60细胞凋亡(英文)

目的:研究环孢菌素(Cyc)在HL-60细胞凋亡中的作用.方法:通过DNA凝胶电泳、细胞形态观察及流式细胞术等方法确定药物三尖杉酯碱(Har), 喜树碱(Cam)或卡西霉素(Cal),thapsigargin(Tha)诱导的细胞凋亡.流式细胞术测定细胞凋亡中胞内相对自由钙浓度的变化.结果:Cal或Tha均能诱导HL-60细胞凋亡,其效应可被Cyc抑制;但Cyc不抑制Hat或Cam诱导的HL-60细胞凋亡.Cal或Tha均能诱导细胞内Ca~(2 )浓度升高,而Hat或Cam并不诱导胞内Ca~(2 )浓度升高.结论:Cyc只抑制由Ca~(2 )升高介导的细胞凋亡.Cal或Tha诱导的细胞凋亡机制不同于由Hat或Cam所诱导的细胞凋亡机制.     

POTENT HYPOTENSIVE EFFECTS OF ORPHANIN FQ IN CONSCIOUS STROKE-PRONE SPONTANEOUSLY HYPERTENSIVE RATS

INTRODUCTIONOrphaninFo(OFQ)ornociceptinisanovelneuropeptidethatactivatesanopioid--likeGprotein--coupledreceptor(1).OFQboundtoitsreceptorinasaturablemannerandwithhighaffinity.Itdidnltshowanyactivityattheopioidreceptor(2).NorthernanalysisofhumanandrattissuesshowedthattheOFQprecusormRNAismainlyexpressedinthecentralnervoussystem(brainandspinalcord)and,albeitweakly,intheovary.Byusingaradiationhybridcelllinepanel,theOFQprecusorgenewasmappedtotheshortarmofhumanchromosome8(spZI)(3,4).Nort…