贵州省陆军某部男性新兵卡特尔16种人格因素分析

目的:了解贵州省陆军某部男性新兵的人格特征状况.方法:以Cattell Raymond B.16项个性因素测评量表(16PF)为工具测试男性新兵157名,并将测评结果与辽宁省1981年男性成人常模进行比较.结果:样本群体在11项人格因素上与成人常模比较差异有显著性.结论:新兵心理健康状况应引起足够重视并完善辅导机制.     

抽动—秽语综合征的治疗体会

抽动-秽语综合征（deleTrouetle’s综合征、TS）。其治疗多首选经典抗精神病药物。我们认为TS的治疗必须是综合性的药物治疗、行为矫正和心理治疗三者联合。现将一典型病例报道如下。患儿男性，14岁，中学生，从4岁开始无明显诱因出现全身各处油动，每天数次至数十次，早轻夜重，同时伴随污秽语言或喉呜。患者出生时曾发生窒息，其原因末明。家族中无类似患者。体格检查与同龄儿童无区别，智力发育一般，各科成绩均在60分左右。情绪不稳定时，有冲动行为。经适应性行为评定为中度不良。在检查过程中经常发现头颈、手足、躯体历时1～2秒的…     

Studies on Expression of P1 Protamine Gene in Rat and Mouse Testis

Protamine is a kind small,basic protein rich in arginine residues and found to be complexed with DNA in spermatozoa. We have cloned a 150 bp cDNA encoding the rat protamine (rP) by RT-PCR technique.Dig-labelled cDNA for rP was used for Northern blot analysis to study the expression of P1 protamine gene in rat and mouse.P1 protamine mRNA was detected only in rat testis,no hybridization signals were de-tected in rat brain and lever.In addition,the presence of P1 protamine mRNA was detected not only in rat testis,but also in mouse testis.Dig-labelled cDNA for mouse protamine 1 (mP1) was used to study the expression of mP1 gene during the process of sexual maturation of mouse.7-8 d after birth,no mP1 mRNA could be detected.At d 24-26,mP1 mRNA was detectable migrating as a homogeneous band at 580 nu-cleotides,whereas in sexually mature animals,a heterogeneous mixture of RNAs ranging from 450-580 bases in length was observed.Histological studies revealed that in the testis of 7-8-day-old mouse, spermatogenesis has developed to the sperma-tocyte stage, whereas round spermatids (Rs) were present in the testis of the mice with 24-26 d age and elongating spermatids(Es)were present in the testis of sexually mature animals. Electrophoresis of total nuclear basic proteins(TNBP)revealed that the Rs could possess the somatic histones,while Es was found to have protamine and less histone.These results indicate that the P1 protamine gene is tissues-specifically ex-pressed and the P1 protamine is showing to be conservative in evolution.During the process of sexual maturation,along with morphological changes,mP1 gene was tran-scribed in Rs and translated in Es.The mechanism of protamine gene expression was discussed.     

奥氮平在抑郁症伴焦虑治疗中的增效作用

奥氮平属于非典型抗精神病类药物，该药对药物难治性的抑郁症、精神病性抑郁、抑郁焦虑综合征、双相情感障碍和强迫症均有良好疗效。现就奥氮平在抑郁症伴焦虑治疗中的增效作用作一探讨，以便为抑郁症伴焦虑患者提供更广泛的治疗途径。     

贵州省某高校新生人格问卷调查分析

目的: 了解贵州省高等学校新生心理健康状况.方法:采用大学生人格问卷(UPI)测试新生731名.结果:可能有严重心理问题的Ⅰ类学生占26.64%,有待关注的Ⅱ类学生占35.81%,心理健康的Ⅲ类学生占37.54%.新生的心理健康状况与性别、城乡无关,与民族、独生子女因素有关,新生选择危急题、危机题比率较高.结论:提示贵州省高校新生心理健康问题值得关注,开展心理卫生工作至关重要.     

贵州省某陆军男性新兵心理健康状况及个性调查

不少文献报告了新兵心理健康情况[1～3].本文对贵州省陆军某部两个连157名男性新兵在入伍后14天进行了首次SCL-90及16PF测试,并对测试结果进行统计分析,现将结果报告如下.     

丝氨酸/苏氨酸蛋白激酶AKT2的体外激酶活性分析

目的 建立蛋白激酶AKT2体外磷酸化检测体系.方法 构建携带AKT2 cDNA编码区的pLNCX2逆转录病毒重组载体,包装重组病毒,转导293A细胞,G418筛选得到稳定表达组成型活化的AKT2细胞株,应用免疫沉淀获得蛋白激AKT2;将核基质结合蛋白SATB1的1～204的氨基酸序列及其47位丝氨酸的突变体S47A、S47D,分别与GST基因融合表达载体pGEX4T-1进行重组,经测序鉴定后转化大肠埃希菌BL-21,IPTG诱导表达经亲和纯化得到GST-SATB1 1-204、GST-SATB1 1-204 S47A和GST-SATB1 1-204 S47D融合蛋白;利用免疫沉淀的AKT2磷酸化GST-SATB1融合蛋白,应用免疫印迹检测其是否被磷酸化.结果 细胞表达的蛋白激酶AKT2能高效的将野生型SATB1 1-204 磷酸化,而不能磷酸化其两种突变体.结论 成功建立了一个蛋白激酶体外磷酸化系统.