氧代赖氨酸抗四氯化碳所致小鼠肝细胞损害的超微结构观察

小鼠ｉｇＣＣｌ４０．１％油溶液１０ｍＬ·ｋｇ－１前ｉｐ氧代赖氨酸５０ｍｇ·ｋｇ－１，每天２次，连续２ｄ，能使ＣＣｌ４所致肝细胞粗面内质网断裂减少，脂肪变性减轻，损伤恢复较快。超微结构观察提示，氧代赖氨酸能引起肝细胞胞浆局部区域溶解，基质丢失。促使ＣＣｌ３失活可能是肝细胞损伤减轻的主要原因。     

日本血吸虫对3H-吡喹酮的摄入与分布

感染小鼠一次口服³H-吡喹酮100mg/kg后,其体内两性血吸虫的³H含量以给药后0.5～1小时为最高,4小时后则有明显减少。体外培养的血吸虫亦能迅速摄取³H-吡喹酮,且其摄入量随药物浓度的增加而递增。体内、体外试验的结果表明,最初进入虫体的³H-吡喹酮系脂溶性,随后在体内缓慢代谢为水溶性。放射自显影的结果说明,³H主要自表皮进入虫体,分布于肌层、间充组织、生殖系统和肠壁上皮细胞,其中以雌虫的卵黄腺为最多,而以雄虫的睾丸为最少。     

MORPHOLOGICAL SURVEY ON ENDOGENOUS C-TYPE VIRUSES INFECTING A HUMAN LUNG SQUAMOUS CARCINOMA PASSAGED IN NUDE MICE

A human lung squamous carcinoma was transplanted and passaged in Swiss-DF nude mice, called LSX-83, for more than five years in our laboratory. The morphological characteristics of the original tumor were maintained in passages from 4 to 33. But from the 35th generation, an increasing amount of tonofilaments and nuclear segregation with typical features was found with electron microscopy. The C-type virus particles were first detected in extra cellular space after 40 passages. The viruses were observed in different stages of growth, but their distribution and number did not show apparent change up to 54 passages. Such findings suggest that LSX-83 cells probably possess certain barrier of resistance against C-type viruses. The relation between C-type viruses and the morphological changes of LSX-83 cells was discussed.     

三株人肺癌裸小鼠移植模型长期传代的形态学变化

对三株人肺癌裸小鼠移植瘤模型作形态学跟踪观察结果显示:人肺燕麦细胞癌连续传代4年后,瘤内小区有柱形细胞;神经性分泌颗粒明显减少;局部呈现腺型分化的倾向。人肺鳞状细胞癌连续传代3年后,上皮大部分分层清楚;张力纤维束分布广泛;核仁微分离典型;分化程度有升级趋势。人肺腺癌连续传代至今已逾4年,微小腺腔作为判别低分化腺癌的特异性结构仍比较原始,形态学无明显改变,但移植瘤从59代起出现淋巴结和肺内转移灶。     

索布佐生和阿霉素对小鼠白血病P388的协同作用

目的:研究索布佐生(Sob)与阿霉素(Dox)联合应用的抑瘤作用以及Sob对Dox诱导的心脏毒性的影响。方法:DBA/2小鼠接种P388细胞,次日起iv Dox 2mg·kg~(-1)·d~(-1)×3d及4mg·kg~(-1)·d~(-1)×1 d,ig Sob 20mg·kg~(-1)·d~(-1)×7d及40mg·kg~(-1)·d~(-1)×7d,配对联用。统计各组的生命延长率(ILS);电镜观察荷瘤鼠垂死前的心肌毒性表现。结果:联合用药组的ILS分别为48.7％,57.3％,59.0％和62.4％,是Sob和Dox单药应用ILS之和的130％—190％,荷瘤鼠心肌细胞的超微结构损伤较单用Dox组明显减轻。结论:Sob与Dox联用对小鼠白血病P388有显著的抑瘤协同作用;Sob能降低Dox所致的心肌毒性。     

日本血吸虫对~3H-吡喹酮的摄入与分布

感染小鼠一次口服~3H-吡喹酮100mg/kg后,其体内两性血吸虫的~3H含量以给药后0.5～1小时为最高,4小时后则有明显减少。体外培养的血吸虫亦能迅速摄取~3H-吡喹酮,且其摄入量随药物浓度的增加而递增。体内、体外试验的结果表明,最初进入虫体的~3H-吡喹酮系脂溶性,随后在体内缓慢代谢为水溶性。放射自显影的结果说明,~3H主要自表皮进入虫体,分布于肌层、间充组织、生殖系统和肠壁上皮细胞,其中以雌虫的卵黄腺为最多,而以雄虫的睾丸为最少。