祁白术对脾气虚模型大鼠的治疗作用*

目的研究祁白术对脾气虚模型大鼠的治疗作用。方法 采用劳倦伤脾加限制饮食的方法建立脾气虚模型大鼠，通过观察不同产地不同剂量的白术汤剂治疗模型大鼠1周后的血清D-木糖含量、胃泌素、胃动素、胃蛋白酶、胰蛋白酶、白细胞介素-6、生长抑制素、脾和胸腺指数的影响。结果 与对照组相比，模型组大鼠的体质量显著下降并且其血清D-木糖含量、胃泌素、胃蛋白酶、胰蛋白酶、脾和胸腺指数水平明显降低，但是白细胞介素-6和生长激素抑制素的水平显著升高。给予模型组大鼠白术汤剂和丹弗胃尔康颗粒后，给药组指标与模型组相反。结论 给予祁白术的模型组大鼠的检测指标变化更为明显。不同产地白术的治疗效果也不同，实验结果表明，祁白术对脾气虚大鼠有治疗作用。     

Development of a method for determination of osmotic pump controlled-release tablets of lorcaserin hydrochloride in beagle dog plasma and its application to pharmacokinetic study北大核心CSCD

Aim To develop an UPLC-MS/MS method to determine the concentration of lorcaserin hydrochloride in beagle plasma, and study the pharmacokinetics of osmotic pump controlled-release tablets of lorcaserin hydrochloride. Methods A randomized crossover design was used, carbamazepine as the internal standard(IS), and plasma protein precipitation with acetonitrile. The chromatographic was Phenomenex Polar C18 column(100 mm×2. 1 mm, 3 μm), and acetonitrile - water(containing 10 mmol·L-1 ammonium acetate and 0.1% formic acid)(40:60, V/V)was mobile phase. Multiple reaction monitoring mode and electrospray positive ionization were used to detect lorcaserin hydrochloride. The MS/MS ion transitions were monitored at m/z 196.2→129.2 for lorcaserin hydrochloride and m/z 237→194.1 for carbamazepine, respectively. Results The linear range was 1 to 500 μg·L-1(r=0.999 2), the extraction recovery rate ranged from 87.70% to 89.70%, the precision RSD was 9.7%. The accuracy and matrix effect met the requirements, and the stability of lorcaserin hydrochloride was good in -20 ℃ refrigerator for 45 d, repeated freezing and thawing for three times, placed at room temperature for 24 h, and the disposed samples placed in automatsampler for 6 h were stable. The main pharmacokinetic parameters of the controlled-release tablet and immediate-release tablet were as follows:Tmax was(8.00±1.27)h and(1.00±0.13)h, Cmax was(70.56±3.73)μg·L-1 and(176.33±16.73)μg·L-1, and AUC0-t was(966.33±7.56)μg·h·L-1 and(973.05±69.09)μg·h·L-1, respectively. Conclusions The established UPLC-MS/MS method can be used to study the pharmacokinetics of lorcaserin hydrochloride in the plasma of beagle dogs, and osmotic pump controlled-release tablets has sustained release effect. © 2023 Publication Centre of Anhui Medical University. All rights reserved.