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本文通过1982年夏季140例婴幼儿胃肠炎的临床分析及病原学的研究,发现本病的临床过程和病原学与过去的认识有所不同。过去认为夏季婴幼儿胃肠炎以致病性大肠杆菌为主要病原;而轮状病毒肠炎主要发生在秋冬寒冷季节,本文材料分析夏季婴幼儿胃肠炎的病原中,致病性大肠杆菌已下降为5.98%,而以病毒病原居多数,占72%,电镜检出轮状病毒颗粒占32%,血清学检查抗轮状病毒抗体阳性占38%。夏季婴幼儿胃肠炎传染性强,抗生素治疗对病毒性肠炎无效。 相似文献
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本刊从本期开始将连续扼要刊载几篇由中华医学会北京分会、北京中医学会和中国中西医结合研究会北京分会等于今夏组织的感染性腹泻防治专题报告会的文章,包括腹泻的病原学研究、流行病学的调查、临床诊断与治疗的进展及各种先进的检查技术等,既有研究价值又有实际意义,对于提高腹泻的预防、诊断、治疗等方面将能有所帮助。希望读者对这种专题讨论的形式、内容和作用等方面广泛提出意见和要求,便于改进工作。 相似文献
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This article deals with studies in late
autumn 1981 0f the infection of wild adult
Tupaia belangeri yunalis (a species of tree shrew
caught in autumn in Yunnan province) with
human rotavirus., 12 tupaias are infected in the
first passage, 3 in the second passage and 3 in
the third passage, 12/12, 2/3 and 2/3 0f the tu-
paias develop diarrhea during the 3 passages.
Electron microscopic observat.ion shoiws that
12/12, 3/3 and 2/3 0f the tupaias excrete rota-
virus during the passages. Three tupaias died
during the first passage and show mucosal ne-
crosis and lymphocyte infiltration in sections of
different intestinal parts. Immune electron mi-
croscopically, anti-rotavirus antibody is assayed
in sera of 2 infected tupaias, in both of which
titer rises greater than 4 fo.lds in the convalescent
phase serum as compared with the pre-infection
serum. These results indicate that the adult
tupaia is probably susceptible to human rota-
virus. 相似文献
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检测轮状病毒,常用ELISA和聚丙烯酰胺凝胶电泳(PAGE)法,但有时出现组间抗原不交叉和电泳图型变异,还要靠电镜检查证实;检测与腹泻有关的其它病毒,由于细胞培养和血清学方法尚未建立,电镜检查仍然是发现和鉴别它们的主要手段。为了捕获急性腹泻病人粪便中可能存在的各种病毒,发展一种具有广谱性和高灵敏性的多价抗体免疫电镜(PAbI-EM)法实属必要。(一)多价抗体的选择 相似文献
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This article reports the detection of small double capsid virus and results of preliminary studies on its morphology, serology and relationship to gastroenteritis. A kind of small virus particles with morphological characteristics was found in fecal samples of 3 of 23 infants with autumn gastroenteritis. The average diameter of this virus particles is 34.1 +/- 1.1 nm, possessing core, double capsids and spoke-like capsomers. It can be differentiated from other viruses in morphology. Correlated antigenicity exists among 3 strains of the virus but not among other studied viruses and this virus. The titer of the antibody to small double capsid virus in convalescent phase-sera of 2 patients was more than 4-fold as high as that in control sera. This virus was absent in fecal samples of recovered patients as well as in those of normal infants. These show that small "double layer like" capsid virus might be one of the etiological agents of acute gastroenteritis. 相似文献