CpG ODN刺激的寻常性银屑病中性粒细胞培养上清液对HaCaT细胞增殖的影响

Objective To investigate the role of nentrophils in the pathogenesis of psoriasis vulgaris. Methods Neutrophils were isolated from venous blood samples of 25 patients with psoriasis vulgaris (including 13 cases of active psoriasis and 12 cases of inactive psoriasis) as well as 25 normal human con-trols, and cultured. Then, these neutrophils were grouped and treated with lipopolysaccharide (LPS, 100 g/L),CpG-A (50 mg/L), CpG-B (50 mg/L), and RPMI 1640 culture medium, respectively, for 24 hours followed by the collection of culture supematants. Human keratinocytes (HaCaT) were cultured in the presence of su-pematants of treated or untreated nentrophils for 72 hours followed by the detection of cell proliferation with MTT assay. To determine the role of proinflammatory factors, SOD/CAT and monoclonal antibody to IL-8 and TNF-alpha of 400 u/mL were used to pretreat HaCaT cells 1 hour prior to the stimulation with super-natants of neutrophils. Results Compared with culture medium, the supematant of unstimulated neutrophils from normal controls or patients with inactive psoriasis had no significant effect on the proliferation of HaCaT cells (P ＞ 0.05), but that from patients with active psoriasis markedly promoted the proliferation of HaCaT cells (t = 2.41, P < 0.05). ARe, stimulation by LPS, CpG-A and CpG-B, the supematant of active patient-derived neutrophils significantly promoted the proliferation of HaCaT cells compared with that of normal control-derived nentrophils (t = 3.11, 2.89, 2.29, respectively, all P < 0.05). In comparison with tmstimulated neutrophils, the supematant from LPS- and CpG-A stimulated nentrophiles significantly accelerated the pro-liferation of HaCaT cells. Furthermore, the proliferation of HaCaT cells induced by the supematants of LPS-,CpG-A-, CpG-B-stimulated neutrophils from psoriatic patients was statistically suppressed by the pretreat-ment with the monoclonal antibody to IL-8, TNF-alpha and SOD/CAT (all P < 0.05). Conclusions In patients with psoriasis vulgaris, there is an abnormal secretion of IL-8, TNF-alpha and superoxide by neutrophils in peripheral blood, and these proinflammatory factors could promote the proliferation of HaCaT cells.     

嗜酸性蜂窝织炎1例

嗜酸性蜂窝织炎（ｅｏｓｉｎｏｐｈｉｌｉｃｃｅｌｌｕｌｉｔｉｓ）是一种少见的嗜酸性细胞增多性疾病,有着独特的临床症状和特殊的组织病理改变。作者曾收治１例,现报告如下。患者女,４２岁。右侧躯干皮肤反复发疹１年余,于１９９７年９月３日入院。１９９６年１月患者在马达加斯加工作期间,无明显诱因右胸腹皮肤出现一５ｃｍ×６ｃｍ大小红斑,１０天后红斑消退,继而同侧锁骨下又出现类似皮损,局部轻度灼热及针刺样感,无全身其它不适,２周后皮损消退。１９９６年１０月回国后又出现一次类似发作。发作期间曾服用先锋类抗生素及抗…     

疤疹样皮炎和成人小肠吸收不良症中组织相容性抗原的比较

疱疹样皮炎(DH)与肠病之间的关系早在1966年已被Marks等首先证明,随即发现肠病是对谷胶过敏。但是对于DH中谷胶过敏性肠病(GSE)的发病率与意义仍有争论,较早的观点认为DH病人有二组:一组约2/3的病人有GSE,仅这些病人像成人小肠吸收不良症(Adult coeliac disease,ACD)一样,HL—A_8的发生率增加;而另一组HL—A_8发生率正常、无肠病。目前的观点相信所有的DH病人都有GSE,而那些较早报道的仅2/3的病人有GSE是由于诊断的标准不适当所造成的。对于DH中皮肤和肠病之间的关系也有二种不同的观点:第一种观点认为这样关系是直接的,皮肤和肠道损害都与谷胶有关;第二种观点认为上述关系是间接的,皮肤损害与谷胶无关。     

CpG ODN刺激的寻常性银屑病中性粒细胞培养上清液对HaCaT细胞增殖的影响

Objective To investigate the role of nentrophils in the pathogenesis of psoriasis vulgaris. Methods Neutrophils were isolated from venous blood samples of 25 patients with psoriasis vulgaris (including 13 cases of active psoriasis and 12 cases of inactive psoriasis) as well as 25 normal human con-trols, and cultured. Then, these neutrophils were grouped and treated with lipopolysaccharide (LPS, 100 g/L),CpG-A (50 mg/L), CpG-B (50 mg/L), and RPMI 1640 culture medium, respectively, for 24 hours followed by the collection of culture supematants. Human keratinocytes (HaCaT) were cultured in the presence of su-pematants of treated or untreated nentrophils for 72 hours followed by the detection of cell proliferation with MTT assay. To determine the role of proinflammatory factors, SOD/CAT and monoclonal antibody to IL-8 and TNF-alpha of 400 u/mL were used to pretreat HaCaT cells 1 hour prior to the stimulation with super-natants of neutrophils. Results Compared with culture medium, the supematant of unstimulated neutrophils from normal controls or patients with inactive psoriasis had no significant effect on the proliferation of HaCaT cells (P ＞ 0.05), but that from patients with active psoriasis markedly promoted the proliferation of HaCaT cells (t = 2.41, P < 0.05). ARe, stimulation by LPS, CpG-A and CpG-B, the supematant of active patient-derived neutrophils significantly promoted the proliferation of HaCaT cells compared with that of normal control-derived nentrophils (t = 3.11, 2.89, 2.29, respectively, all P < 0.05). In comparison with tmstimulated neutrophils, the supematant from LPS- and CpG-A stimulated nentrophiles significantly accelerated the pro-liferation of HaCaT cells. Furthermore, the proliferation of HaCaT cells induced by the supematants of LPS-,CpG-A-, CpG-B-stimulated neutrophils from psoriatic patients was statistically suppressed by the pretreat-ment with the monoclonal antibody to IL-8, TNF-alpha and SOD/CAT (all P < 0.05). Conclusions In patients with psoriasis vulgaris, there is an abnormal secretion of IL-8, TNF-alpha and superoxide by neutrophils in peripheral blood, and these proinflammatory factors could promote the proliferation of HaCaT cells.     

寻常型银屑病患者中性粒细胞分泌炎性因子的研究

目的观察寻常型银屑病患者外周血中性粒细胞是否存在分泌炎性因子的异常,探讨中性粒细胞参与银屑病的机制。方法作中性粒细胞培养,应用酶联免疫吸附法检测脂多糖(LPS)刺激前后中性粒细胞分泌白细胞介素-8(IL-8)、肿瘤坏死因子-α(TNF-α)的水平,应用细胞色素C还原法检测LPS刺激前后中性粒细胞超氧阴离子(Superox-ide anion)的水平。结果在脂多糖刺激前、后,银屑病患者外周血中性粒细胞分泌的IL-8、TNF-α以及Superoxide anion水平均较正常人显著增高(P<0.05),且进行期患者上述炎性因子的分泌水平显著高于静止期患者(P<0.01)。银屑病患者病情严重程度(PASI评分)与中性粒细胞于脂多糖刺激前、后IL-8、TNF-α及Superoxideanion水平均呈正相关(P<0.05)。结论银屑病患者存在外周血中性粒细胞分泌炎性因子的异常,该种异常可能参与银屑病的发病与病情进展,其中可能有感染因素的介导。     

CpG ODN刺激的寻常性银屑病中性粒细胞培养上清液对HaCaT细胞增殖的影响

Objective To investigate the role of nentrophils in the pathogenesis of psoriasis vulgaris. Methods Neutrophils were isolated from venous blood samples of 25 patients with psoriasis vulgaris (including 13 cases of active psoriasis and 12 cases of inactive psoriasis) as well as 25 normal human con-trols, and cultured. Then, these neutrophils were grouped and treated with lipopolysaccharide (LPS, 100 g/L),CpG-A (50 mg/L), CpG-B (50 mg/L), and RPMI 1640 culture medium, respectively, for 24 hours followed by the collection of culture supematants. Human keratinocytes (HaCaT) were cultured in the presence of su-pematants of treated or untreated nentrophils for 72 hours followed by the detection of cell proliferation with MTT assay. To determine the role of proinflammatory factors, SOD/CAT and monoclonal antibody to IL-8 and TNF-alpha of 400 u/mL were used to pretreat HaCaT cells 1 hour prior to the stimulation with super-natants of neutrophils. Results Compared with culture medium, the supematant of unstimulated neutrophils from normal controls or patients with inactive psoriasis had no significant effect on the proliferation of HaCaT cells (P ＞ 0.05), but that from patients with active psoriasis markedly promoted the proliferation of HaCaT cells (t = 2.41, P < 0.05). ARe, stimulation by LPS, CpG-A and CpG-B, the supematant of active patient-derived neutrophils significantly promoted the proliferation of HaCaT cells compared with that of normal control-derived nentrophils (t = 3.11, 2.89, 2.29, respectively, all P < 0.05). In comparison with tmstimulated neutrophils, the supematant from LPS- and CpG-A stimulated nentrophiles significantly accelerated the pro-liferation of HaCaT cells. Furthermore, the proliferation of HaCaT cells induced by the supematants of LPS-,CpG-A-, CpG-B-stimulated neutrophils from psoriatic patients was statistically suppressed by the pretreat-ment with the monoclonal antibody to IL-8, TNF-alpha and SOD/CAT (all P < 0.05). Conclusions In patients with psoriasis vulgaris, there is an abnormal secretion of IL-8, TNF-alpha and superoxide by neutrophils in peripheral blood, and these proinflammatory factors could promote the proliferation of HaCaT cells.     

职业性皮肤病的诊断

职业性皮肤病,是指在劳动中以化学、物理、生物等职业性有害因素为发病的主要原因引起的皮肤及其附属器的疾病。其中以工业职业性皮肤病发病率最高,根据各国不同地区的统计资料,其发病率占整个工业职业病总数的55～80%。在职业性皮肤病的发病原因中,以化学     

20——甲营养不良一例报告

<正> 病历摘要男患,5岁。因20甲全部变形、增厚,表面凸凹不平,失去光泽。于1988年7月就诊。患儿出生时20甲全部正常,于生后三个月发现10个指甲相继变成红褐色、增厚,继之10个趾甲也出现同样现象。先从拇指开始,指甲由红褐色至甲下出血最后指(趾)甲脱落。再生后的指甲仍然粗糙,无光泽,增厚,有明显的纵嵴。其母孕期无患病史或服药史。患儿系足月顺产第二胎。第一胎生产时死亡。出生后未发现指甲改变。患儿各生长发育期正常。其父母亲非近亲婚配,无家族遗传性疾病。查体:发育正常,营养中等,     

CpG ODN刺激的寻常性银屑病中性粒细胞培养上清液对HaCaT细胞增殖的影响

Objective To investigate the role of nentrophils in the pathogenesis of psoriasis vulgaris. Methods Neutrophils were isolated from venous blood samples of 25 patients with psoriasis vulgaris (including 13 cases of active psoriasis and 12 cases of inactive psoriasis) as well as 25 normal human con-trols, and cultured. Then, these neutrophils were grouped and treated with lipopolysaccharide (LPS, 100 g/L),CpG-A (50 mg/L), CpG-B (50 mg/L), and RPMI 1640 culture medium, respectively, for 24 hours followed by the collection of culture supematants. Human keratinocytes (HaCaT) were cultured in the presence of su-pematants of treated or untreated nentrophils for 72 hours followed by the detection of cell proliferation with MTT assay. To determine the role of proinflammatory factors, SOD/CAT and monoclonal antibody to IL-8 and TNF-alpha of 400 u/mL were used to pretreat HaCaT cells 1 hour prior to the stimulation with super-natants of neutrophils. Results Compared with culture medium, the supematant of unstimulated neutrophils from normal controls or patients with inactive psoriasis had no significant effect on the proliferation of HaCaT cells (P ＞ 0.05), but that from patients with active psoriasis markedly promoted the proliferation of HaCaT cells (t = 2.41, P < 0.05). ARe, stimulation by LPS, CpG-A and CpG-B, the supematant of active patient-derived neutrophils significantly promoted the proliferation of HaCaT cells compared with that of normal control-derived nentrophils (t = 3.11, 2.89, 2.29, respectively, all P < 0.05). In comparison with tmstimulated neutrophils, the supematant from LPS- and CpG-A stimulated nentrophiles significantly accelerated the pro-liferation of HaCaT cells. Furthermore, the proliferation of HaCaT cells induced by the supematants of LPS-,CpG-A-, CpG-B-stimulated neutrophils from psoriatic patients was statistically suppressed by the pretreat-ment with the monoclonal antibody to IL-8, TNF-alpha and SOD/CAT (all P < 0.05). Conclusions In patients with psoriasis vulgaris, there is an abnormal secretion of IL-8, TNF-alpha and superoxide by neutrophils in peripheral blood, and these proinflammatory factors could promote the proliferation of HaCaT cells.     

影响银屑病患者心理健康水平的社会因素

目的探讨影响银屑病患者心理健康状况的有关社会因素.方法采用症状自评量表及自拟的有关社会因素,以标准问卷的形式由患者自评,通过199例有效问卷的调查和统计分析,比较两者之间的关系.结果小学文化程度与大专以上文化程度的患者,在焦虑、敌意两方面差异显著(P<0.05);个人收入较低者与中等及较高收入患者组在强迫、人际敏感、抑郁和精神病性四方面均存在显著差异(P<0.05);人际关系一般的患者与良好的患者除躯体化、偏执、精神病性三方面差异不显著外,其余因子均有显著差异(P<0.05).结论注意改善人际关系、经济状况、文化程度等社会因素,有助于银屑病患者心理健康水平的提高.