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脑出血和脑梗死均属于中医学的“中风”范畴,是目前世界上导致人类死亡的第2位原因,也是单病种致残率最高的疾病.在我国脑血管病已跃升为国民死因的首位。本病的高发病率、高死亡率和高致残率给社会、家庭和患者带来沉重的负担和巨大的痛苦[1]。发病后的2周至6个月为中风恢复期[2]。中风恢复期是患者肢体功能恢复的重要时期,治疗得当与否。决定着患者预后的好坏。近年来,中医药治疗中风恢复期的临床研究日渐增多,疗效显著。 相似文献
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In this study, the effects of pirrolidine dithiocarbamate (PDTC) plus leflunomide (Lef) and cyclosporine (CsA) on the NF-κB signaling pathway in mouse-to-rat cardiac xeno-transplantation models were investigated. NIH mice and Wistar rats served as donors and recipients respectively. Mouse-to-rat cardiac xenotransplantation was performed. The recipients were divided into 5 groups: group A (the control group), group B (PDTC group), group C (PDTC plus CsA group), group D (PDTC plus Lef group) and group E (PDTC plus Lef and CsA group). The expressions of IKKa/[3, NF-κB-P65, IκBct, ICAM-1 and NF-κB DNA binding activity in xenograft tissues were determined by immunohistochemistry and Western blot as well as electrophoretic mobility shift assay (EMSA). The median survival time of cardiac xenografts in the control group, PDTC group, PDTC plus CsA group, PDTC plus Lef group and PDTC plus Lef and CsA group was (2.17±0.41), (2.33±0.52), (4.67±1.21), (7.00±1.79) and (9.00±1.41) days respectively. The survival time of xenografts in the PDTC plus Lef and CsA group was significantly longer than that in other four groups (P〈0.05 for all), that in the PDTC plus Lef group longer than that in the control group, PDTC group and PDTC plus CsA group (P〈0.05 for all), that in PDTC plus CsA group longer than the control group and PDTC group (P〈0.05 for all). The expressions of IKKα/β, NF-κB-P65, IκBα and ICAM-1 and NF-κ3 DNA binding activity were notably increased in mouse-to-rat cardiac xenografts. The expressions were decreased in the control group, PDTC group, PDTC plus CsA group, PDTC plus Lef and PDTC plus Lef and CsA group in turn. It was concluded that PDTC plus Lef and CsA can significantly suppress the expressions of IKKα/β, NF-κB-P65, IκBα, ICAM-1 and NF-κB DNA binding activity, thereby prolonging the survival of the cardiac xenografts. 相似文献
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目的采用改良袖套法建立小鼠→大鼠颈部异位异种心脏移植动物模型,动态观察移植心脏病理特征。方法NIH小鼠和Wistar大鼠作为供、受体,用聚乙烯硬膜外麻醉导管和静脉留置针管制作带短臂动、静脉套管,将小鼠升主动脉、肺动脉分别与大鼠右颈总动脉、右颈外静脉端端套入吻合,建立小鼠→大鼠颈部异位心脏移植模型。在移植术后3h(n=4)、8h(n=4)、16h(n=4)、24h(n=4)供心仍跳动时取心,另一组(n=16)在供心停跳时取心并以此决定移植心脏存活时间,未移植小鼠心脏设为对照组(n=4),各组标本作组织病理学检查。结果移植手术40次,成功32次,移植成功率80.0%。移植心脏平均存活时间(49.3±16.2)h。移植心脏病理检查表现为血管内皮损伤、血栓形成、心肌实质损伤、间质出血、炎症细胞浸润等特征随移植术后时间逐渐加重趋势,完全排斥时部分心肌凝固性坏死及溶解、广泛间质出血、血管结构崩解及血管内血栓形成、严重炎症细胞浸润。结论改良袖套法建立鼠颈部异位心脏移植模型操作简便、易于观察、稳定可靠,是研究异种移植较理想的动物模型。动态观察组织病理学特征可为异种移植排斥反应诊断提供依据。 相似文献
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目的建立豆制品中非法添加的15种工业染料超快速液相色谱串联四级杆质谱的快速监测方法。方法样品用乙腈作为提取液,过0.22μm的滤膜直接经C18柱梯度洗脱,正离子模式流动相为甲醇和0.1%的甲酸水溶液,负离子模式流动相为甲醇和纯水。采用多反应监测(MRM)模式进行检测,其中13种化合物设定为正离子模式,2种化合物为负离子模式。采用基质匹配的外标方法进行定量。结果豆干中15种工业染料的检出限为0.2—10μg/kg、定量限为0.6—30μg/kg、回收率为60.1%~113.5%、相对标准偏差为1.1%~14.9%。结论该方法检测时间短,灵敏度高,操作简便,同时用正负离子检测15种不同的工业染料在豆制品中的添加情况,可以根据不同要求添加新的工业染料,同时也可以根据要求变化基质检测其他食品。 相似文献
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