维生素D受体基因启动子区CDX2多态性与儿童氟斑牙的关系

目的 了解维生素D受体(VDR)基因启动子区CDX2结合位点多态性与儿童氟斑牙易感性的关系.方法 Dean法检查8～12岁风台县关店乡儿童氟斑牙患病情况,收集氟斑牙患者102例及正常对照者107例,采集外周静脉血提取DNA,采用等位基因特异多重聚合酶链反应(ASM-PCR)测定两组儿童VDR基因CDX2结合位点多态性.结果 VDR基因CDX2结合位点多态性分布频率为:氟斑牙患者GG占48.0%、AG占25.5%、AA占26.5%;正常对照组GG占55.1%、AG占28.0%、AA占16.9%.两组基因型分布比较差异无统计学意义(P＞0.05).结论 VDR基因CDX2结合位点多态性与所调查儿童的氟斑牙发生无明显关系.     

热牙胶系统2种根管充填方法的临床效果比较

目的 比较热牙胶充填系统2种根管充填方法的临床效果。方法 选择2013年5月至2016年5月在安徽医科大学第二附属医院口腔科因牙髓病、根尖周病需行根管治疗的患者83例,共83颗患牙,按随机数字表法分为两组,试验组42例患者,42颗患牙（119个根管）,对照组41例患者,41颗患牙（111个根管）。试验组患者采用热牙胶连续波加压充填+冷牙胶侧方加压法进行根管充填,对照组患者采用单纯热牙胶连续波加压充填法。比较2组患者充填方法在临床操作中容易出现的失误率（携热器带出牙胶尖、根管内气泡）、根充恰填率以及1年后临床成功率。结果 试验组患者携热器向上拔出时牙胶尖带出根管数6根,对照组43根,差异有统计学意义（P<0.05）。根管内气泡实验组5根,对照组11根,差异无统计学意义（P>0.05）。试验组根管恰填113根,对照组根管恰填74根,差异有统计学意义（P<0.05）。根管治疗术后1年复查,试验组患者临床成功率为95.24%,对照组为85.37%,两组差异无统计学意义（P>0.05）。结论 2种根管充填方法均能达到良好的临床效果,热牙胶连续波加压充填联合冷牙胶侧方加压法较单纯热牙胶连续波加压法充填根管操作失误少、根尖封闭效果更好。     

维生素D受体基因多态性与汉族儿童氟斑牙易感性的关系研究

objective To observe the distribution of vitamin D receptor(VDR)gene polymorphisms in children of Han nationality and investigate the relationship between VDR gene polymorphisms and the susceptibility to children's dental fluorosis of Han nationality.Methods From October of 2008 to March of 2009,a case-control study was conducted among children between 8 and 12 years old with(n=101)and without(n=102)dental fluorosis using Dean method in Guandian countyside of Fengtai county in Anhui province.DNA was extracted from blood samples ofthese children.The Apa I,Bsm I,Fok I and raq I polymorphisms in the VDR gene were genotyped using polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP).The distribution of the genotypes in patients and the control group were analyzed. Results Different genotypes of the VDR gene existed in children of Han nationality with the highest distribution frequency of Aa, bb, Ff, TT, which respectively was 51.7% ( 105/203 ), 89.7% (182/203), 52.7% (107/203), 93.1% (189/203), followed by genotype distribution frequency of aa, Bb, FF, Tt, being 39.9% (81/203),7.9% (16/203),31.5% (64/203) ,6.9% (14/203), respectively. AA,BB, ff, tt distribution frequency was the lowest as follows, 8.4% ( 17/203 ), 2.4% (5/203), 15.8% (32/203),0 (0/203). The frequency distribution of VDR Apa I genotype was AA 7.9% (8/101), Aa 55.4% (56/101), aa 36.7%(37/101) in children with fluorosis, and AA 8.8% (9/102), Aa 48.0% (49/102), aa 43.3% (44/102) in children without fluorosis, respectively. There were no significant differences in the two groups(χ2= 1.13, P > 0.05).The frequency distribution of VDR Bsm I genotype was BB 3.0%(3/101), Bb 5.9%(6/101 ), bb 91.1% (92/101) in children with fluorosis, and BB 2.0% (2/102), Bb 9.8% (10/102), bb 88.2% (90/102) in children without fluorosis, respectively. There were no significant differences in the two groups(χ2 = 0.55, P > 0.05). The frequency distribution of VDR Fok I genotype was FF 28.7%(29/101), Ff 56.4% (57/101), ff 14.9%(15/101) in children with fluorosis, and FF 34.3% (35/102), Ff 49.0% (50/102), ff 16.7% (17/102) in children without fluorosis,respectively. There were no significant differences in the two groups(χ2 = 1.14, P > 0.05). The frequency distribution of VDR Taq I genotype was TT 93.1%(94/101), Tt 6.9%(7/101) in children with fluorosis, and TT 93.1% (95/102), Tt 6.9%(7/102) in children without fluorosis, respectively. The tt genotype was not found. There were no significant differences in the two groups (χ2 = 0.00, P > 0.05 ). Conclusions Different genotypes of the VDR gene existed in children of Han nationality. There were no correlation between VDR Apa I , Bsm I , Fok I , Taq I gene polymorphisms and children's dental fluorosis of Han nationality in this area.     

数字化技术在口腔修复中的应用研究

目的 研究数字化技术在口腔修复中的应用。方法 选取2018年6月—2021年10月期间在安徽医科大学第二附属医院口腔科进行修复治疗的90例患者作为研究对象,随机分为研究组和对照组,每组45例。研究组患者采用口腔数字化技术进行口腔修复,对照组患者则采用传统修复技术进行口腔修复。研究后期分别对两组患者口腔情况进行检查,并设计相关满意度问卷调查表进行统计。结果 研究组出现食物嵌塞和边缘性龈炎的不良现象发生率为4.4%,低于对照组的17.8%,差异有统计学意义(χ²=4.050,P<0.05);研究组患者咬合功能评分高于对照组,差异有统计学意义(t=33.686,P<0.05);研究组的色差发生率为2.2%,低于对照组的17.8%,差异有统计学意义(χ²=4.444,P<0.05);研究组患者满意度为97.8%,高于对照组的77.8%,差异有统计学意义(χ²=8.839,P<0.05)。结论 采用数字化技术对患者进行口腔修复,能减少修复后食物嵌塞,边缘性龈炎等不良情况的发生,咬合功能恢复良好,修复体颜色接...     

种植义齿修复美学区单个牙齿的软组织美学评价

目的评价牙种植体支持的单冠修复牙列缺损的软组织美学效果及患者的满意度。方法观察16例患者21颗单个牙种植体支持的冠修复体周围的软组织状况并与邻牙或对侧同名牙相比较,用PES计分标准进行计分,并对患者的满意度进行调查。结果 PES得分最高14分,最低10分,平均得分12.35±1.05,其中,软组织的质地平均得分最高,为1.97±0.14,软组织水平得分最低,为1.49±0.42,94%患者对修复结果表示满意,6%患者表示基本满意。PES评分结果与患者满意度调查结果呈正相关关系（相关系数r=0.809）,结果具有统计学意义（P〈0.001）。结论美学区种植修复能够满足患者对美观的要求,PES计分标准可以客观地对种植修复的软组织美学效果进行评价。     

热牙胶系统2种根管充填方法的临床效果比较

目的比较热牙胶充填系统2种根管充填方法的临床效果。方法选择2013年5月至2016年5月在安徽医科大学第二附属医院口腔科因牙髓病、根尖周病需行根管治疗的患者83例,共83颗患牙,按随机数字表法分为两组,试验组42例患者,42颗患牙(119个根管),对照组41例患者,41颗患牙(111个根管)。试验组患者采用热牙胶连续波加压充填+冷牙胶侧方加压法进行根管充填,对照组患者采用单纯热牙胶连续波加压充填法。比较2组患者充填方法在临床操作中容易出现的失误率(携热器带出牙胶尖、根管内气泡)、根充恰填率以及1年后临床成功率。结果试验组患者携热器向上拔出时牙胶尖带出根管数6根,对照组43根,差异有统计学意义(P <0. 05)。根管内气泡实验组5根,对照组11根,差异无统计学意义(P> 0. 05)。试验组根管恰填113根,对照组根管恰填74根,差异有统计学意义(P <0. 05)。根管治疗术后1年复查,试验组患者临床成功率为95. 24%,对照组为85. 37%,两组差异无统计学意义(P>0. 05)。结论 2种根管充填方法均能达到良好的临床效果,热牙胶连续波加压充填联合冷牙胶侧方加压法较单纯热牙胶连续波加压法充填根管操作失误少、根尖封闭效果更好。     

维生素D受体基因多态性与汉族儿童氟斑牙易感性的关系研究

objective To observe the distribution of vitamin D receptor(VDR)gene polymorphisms in children of Han nationality and investigate the relationship between VDR gene polymorphisms and the susceptibility to children's dental fluorosis of Han nationality.Methods From October of 2008 to March of 2009,a case-control study was conducted among children between 8 and 12 years old with(n=101)and without(n=102)dental fluorosis using Dean method in Guandian countyside of Fengtai county in Anhui province.DNA was extracted from blood samples ofthese children.The Apa I,Bsm I,Fok I and raq I polymorphisms in the VDR gene were genotyped using polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP).The distribution of the genotypes in patients and the control group were analyzed. Results Different genotypes of the VDR gene existed in children of Han nationality with the highest distribution frequency of Aa, bb, Ff, TT, which respectively was 51.7% ( 105/203 ), 89.7% (182/203), 52.7% (107/203), 93.1% (189/203), followed by genotype distribution frequency of aa, Bb, FF, Tt, being 39.9% (81/203),7.9% (16/203),31.5% (64/203) ,6.9% (14/203), respectively. AA,BB, ff, tt distribution frequency was the lowest as follows, 8.4% ( 17/203 ), 2.4% (5/203), 15.8% (32/203),0 (0/203). The frequency distribution of VDR Apa I genotype was AA 7.9% (8/101), Aa 55.4% (56/101), aa 36.7%(37/101) in children with fluorosis, and AA 8.8% (9/102), Aa 48.0% (49/102), aa 43.3% (44/102) in children without fluorosis, respectively. There were no significant differences in the two groups(χ2= 1.13, P > 0.05).The frequency distribution of VDR Bsm I genotype was BB 3.0%(3/101), Bb 5.9%(6/101 ), bb 91.1% (92/101) in children with fluorosis, and BB 2.0% (2/102), Bb 9.8% (10/102), bb 88.2% (90/102) in children without fluorosis, respectively. There were no significant differences in the two groups(χ2 = 0.55, P > 0.05). The frequency distribution of VDR Fok I genotype was FF 28.7%(29/101), Ff 56.4% (57/101), ff 14.9%(15/101) in children with fluorosis, and FF 34.3% (35/102), Ff 49.0% (50/102), ff 16.7% (17/102) in children without fluorosis,respectively. There were no significant differences in the two groups(χ2 = 1.14, P > 0.05). The frequency distribution of VDR Taq I genotype was TT 93.1%(94/101), Tt 6.9%(7/101) in children with fluorosis, and TT 93.1% (95/102), Tt 6.9%(7/102) in children without fluorosis, respectively. The tt genotype was not found. There were no significant differences in the two groups (χ2 = 0.00, P > 0.05 ). Conclusions Different genotypes of the VDR gene existed in children of Han nationality. There were no correlation between VDR Apa I , Bsm I , Fok I , Taq I gene polymorphisms and children's dental fluorosis of Han nationality in this area.     

13～23岁下颌第二前磨牙单根管锥度的初步研究

目的探讨青少年下颌第二前磨牙单根管的初始锥度。方法收集牙体完整的13～23岁单根管离体下颌第二前磨牙23颗,采用显微CT对标本从根尖向冠方进行断层扫描,将每个根管平均分为3段,通过3ddoctor软件测量根管的近远中向管径及颊舌向管径,计算这3个水平段颊舌向和近远中向根管锥度。结果根管近远中向锥度由根冠至根尖分别为0.06、0.08、0.07,颊舌向锥度由根冠至根尖分别为0.32、0.21、0.25。颊舌向3段锥度之间的差异均有统计学意义（P<0.05）,而近远中向3段锥度之间的差异无统计学意义（P>0.05）。结论13～23岁单根管下颌第二前磨牙根管颊舌向锥度变化明显,最小的锥度在根中1/3。近远中向锥度较颊舌向小,但根管上下差别不大。     

固定位置Er,Cr: YSGG激光对根管壁玷污层去除效果的研究

目的光纤头固定在根管入口处比较不同功率的Er, Cr:YSGG激光活化荡洗(LAI)对根管壁上玷污层的去除效果。方法收集48颗无龋坏单根管恒前牙,按处理方法的不同随机均分为4组(n=12):0.5 W LAI组、1.0 W LAI组、超声荡洗组和注射冲洗组,冲洗结束后将牙纵向劈开分成两份,随机选取其中一份进行电镜扫描,分别扫描根管壁的冠部1/3、根中1/3、根尖1/3,并对残留玷污层用Hülsmann的评分系统进行评分,比较4组冲洗方法对玷污层去除效果。结果在冠部1/3,1.0 W LAI组清除玷污层比注射冲洗组和超声荡洗组更为有效(P<0.05),在根中1/3和根尖1/3,1.0 W LAI组与超声荡洗组之间差异无统计学意义(P>0.05),但比其他两组更为有效(P<0.05)。结论光纤头置于根管入口处且功率为1.0 W的Er, Cr:YSGG激光LAI可有效去除玷污层,且优于0.5 W LAI组和注射冲洗组,并在冠部1/3优于超声荡洗组。     

四种暂封材料对根管治疗后冠方微渗漏的影响

背景:临床上需要进行根管治疗的牙齿多数有较大牙体缺损,甚至是残冠、残根,而且永久性修复体需要在治疗后一段时间才能完成,故根管治疗后冠修复前暂时封固对冠方微渗漏的影响已经被临床医生所重视。目的:评价不同暂封材料对根管治疗后牙冠方微渗漏的影响,探讨根管治疗后牙冠方微渗漏发生情况及与时间的关系。方法:收集人离体牙126颗,其中120颗为实验组,3颗为阳性对照组,3颗为阴性对照组。实验组和阳性对照组牙齿进行根管治疗,而后采用牙齿牙胶尖和AH-plus根管糊剂冷侧压充填实验组根管,随机均分为4组,分别以Coltosol、Caviton、ZOE、Ceivitron暂封材料暂封根管治疗后的牙冠方,阳性对照组不充填根管,阴性对照组牙齿保持完整。分别于暂封1,2,4周观察各组样本染料微渗漏情况。结果与结论:阴性对照组未见染料渗入根管,阳性对照组可见整个根管被染色。暂封1周时,Coltosol组、Caviton组微渗漏长度短于ZOE组和Ceivitron组(P<0.05);暂封2周时,Coltosol组微渗漏长度短于Ceivitron组(P<0.05);暂封4周时,Coltosol组、Caviton组和ZOE组微渗漏长度短于Ceivitron组(P<0.05)。随暂封时间的延长,4种材料组微渗漏长度均增加,组内不同时间点微渗漏长度差异均有显著性意义(P<0.05)。结果表明Coltosol和Caviton材料的暂封效果较好,其次为ZOE,Ceivitron最差,Coltosol和Caviton用于暂封的适宜时间为一二周,ZOE和Ceivitron为1周。