复方四黄栓的定性定量方法研究

目的建立复方四黄栓的定性定量方法。方法采用薄层色谱法对大黄、白及、五倍子进行定性鉴别;采用高效液相色谱法同时测定制剂中盐酸小檗碱、黄芩苷的含量。Dikma Diamonsil C18色谱柱(250 mm×4.6 mm),流动相为乙腈-0.02 mol L-1磷酸二氢钾,梯度洗脱,检测波长278 nm(黄芩苷)、345 nm(盐酸小檗碱),柱温25℃,流速1.0 mL min-1。结果 TLC鉴别方法专属性强;盐酸小檗碱在进样量0.238～1.19μg线性关系良好,黄芩苷在进样量0.16～0.80μg线性关系良好,r=0.999 9。盐酸小檗碱和黄芩苷加样回收率分别为98.9%、99.7%,RSD分别为1.5%、1.7%(n=6)。结论本方法操作简便,结果准确,重复性好,可有效控制复方四黄栓的质量。     

五倍子配方颗粒中鞣质含量比较

目的：对不同厂家的五倍子配方颗粒进行鞣质的含量测定,同时对此方法进行讨论,以期为五倍子配方颗粒的质量标准建立提供依据。方法：利用紫外分光光度法对不同厂家的五倍子配方颗粒进行鞣质含量测定。结果：鞣质含量测定结果分别为51.80%,51.18%,51.75%,53.51%,50.97%,50.43%,51.46%,51.93%,51.37%,51.31%。没食子酸线性回归方程：y=0.1128x＋0.0492,r=0.9999,线性范围：1.003 0～10.030 0μg/mL。结论：不同厂家各批次配方颗粒鞣质含量均超过50%,符合2005版中国药典规定,测定结果合格。     

Inhibitory effect of diosgenin on experimentally induced benign prostatic hyperplasia in rats

This study investigated the effect of diosgenin, a natural sapogenin possessing various pharmacological activities, on benign prostatic hyperplasia (BPH) in rats and the possible mechanisms. BPH was established in the castrated rats by subcutaneous injection of testosterone propionate. Animals were randomly divided into four groups (n=10 each): model group (0.5% sodium carboxymethyl cellulose); positive control group (3 mg/kg finasteride); two diosgenin groups (50 and 100 mg/kg). The drugs were intragastricaly given in each group for consecutive 3 weeks. Another 10 rats with no testicles cut off served as negative controls and they were subcutaneously injected with 0.1 mL olive oil per day and then treated with 0.5% sodium carboxymethylcellulose. After 3-week administration, the prostate index and serum PSA level were determined, and histopathological examination was carried out. The levels of MDA, SOD and GPx in prostates were also measured. Additionally, the expression of Bcl-2, Bax and p53 was examined using Western blotting. The results showed that the prostate index and serum PSA level were significantly decreased, and the pathological changes of the prostate gland were greatly improved in diosgenin groups as compared with the model group. Elevated activities of SOD and GPx, and reduced MDA level were also observed in diosgenin-treated rats. In addition, the expression of Bcl-2 in prostates was down-regulated, whereas that of Bax and p53 was up-regulated in diosgenin-treated rats. These results indicated that diosgenin was effective in inhibiting testosterone propionate-induced prostate enlargement and may be a candidate agent for the treatment of BPH.     

高效液相色谱法测定复方樟脑醑中樟脑的含量

目的：建立高效液相色谱法测定复方樟脑醑中樟脑的含量。方法：采用Agilent1200高效液相色谱仪,色谱柱为Diamonsil C18柱,流动相为甲醇-0．2％磷酸水溶液（60：40）,流速为1．0mL／min,检测波长为289nm。结果：樟脑浓度在0．2—1．6mg／mL范围内与峰面积呈良好线性关系（R^2=0．9993）,平均回收率为99．39％。结论：本方法准确、简便、重现性好,可用于复方樟脑醑中樟脑的含量测定。     

五倍子配方颗粒中鞣质含量比较

目的:对不同厂家的五倍子配方颗粒进行鞣质的含量测定,同时对此方法进行讨论,以期为五倍子配方颗粒的质量标准建立提供依据。方法:利用紫外分光光度法对不同厂家的五倍子配方颗粒进行鞣质含量测定。结果:鞣质含量测定结果分别为51.80%,51.18%,51.75%,53.51%,50.97%,50.43%,51.46%,51.93%,51.37%,51.31%。没食子酸线性回归方程:y=0.1128x+0.0492,r=0.9999,线性范围:1.003 0～10.030 0μg/mL。结论:不同厂家各批次配方颗粒鞣质含量均超过50%,符合2005版中国药典规定,测定结果合格。     

独脚金药材的质量标准

目的：初步建立独脚金药材的质量标准。方法：采用薄层色谱对独脚金进行定性鉴别,采用高效液相色谱法测定独脚金中芹菜素的含量。结果：薄层色谱分离效果好、斑点清晰;芹菜素对照品的进样量在1．25～12．5μg（r＝0．9997）范围内峰面积积分值呈良好线性关系;平均加样回收率为98．71％,RSD=1．56％（n=6）。结论：所建立的质量标准有利于对独脚金药材的质量控制。     

首乌生发颗粒对小鼠的急性毒性实验研究

目的 :观察将首乌生发颗粒经口灌胃小鼠后产生的急性毒性实验 ,考察该制剂临床用药的安全性。方法 :采用灌胃给药后 ,观察并记录小鼠中毒症状和死亡数 ,按生药量计算的小鼠最大耐受量。结果 :试验期间小鼠活动状况良好 ,未出现不良反应和死亡情况。结论 :试验结果表明小鼠对本品最大耐受量 >186g·kg-1,约相当于临床给药剂量的 2 34倍。说明本品毒性极低 ,安全性好。     

高效液相色谱法同时测定麻仁丸中大黄素、大黄酚、大黄酸、大黄素甲醚、芦荟大黄素的含量

目的建立麻仁丸中大黄素、大黄酚、大黄酸、大黄素甲醚、芦荟大黄素的含量测定方法。方法采用奥泰ALLTIMA-C18色谱柱(4.6 mm×250 mm,5μm),以甲醇(A)-0.1%磷酸溶液(B)为流动相,梯度洗脱[0~9min,60%A;9~20 min,60%→80%A;20~45 min,80%A];流速:1.0 mL.min-1;柱温:30℃;检测波长254 nm。结果大黄素、大黄酸、大黄酚、大黄素甲醚、芦荟大黄素线性范围分别为在9.08~81.72、8.4~75.6、13.42~120.7、7.56~68.04、8.2~73.8μg.mL-1,与峰面积线性关系良好,平均回收率分别为99.1%、99.6%、99.4%、99.8%、99.2%,RSD分别为2.0%、1.8、3.2%、1.1%、1.5%。结论本方法可作为麻仁丸中大黄素、大黄酚、大黄酸、大黄素甲醚、芦荟大黄素含量测定的一种准确、灵敏、可行的方法。     

续断配方颗粒中川续断皂苷Ⅵ含量比较

目的:测定不同厂家续断配方颗粒中川续断皂苷Ⅵ含量,为制定最佳提取工艺和统一的质量控制方法提供依据。方法:采用HPLC法测定,色谱柱为Diamonsil C18(2)(250 mm×4.6 mm,5μm),流动相乙腈-0.2%磷酸溶液梯度洗脱,检测波长212 nm,流速1 ml.min-1,柱温30℃。结果:川续断皂苷Ⅵ在0.603～3.016μg范围内呈良好的线性关系;供试品溶液在24 h内稳定,RSD为4.78%;加样回收率为99.49%(RSD=1.86%)。结论:所建立的方法适用于续断配方颗粒中川续断皂苷Ⅵ的含量测定。     

凉粉草药材生产质量的指纹图谱评价研究

目的:运用指纹图谱技术,对不同采收月份、采收时间和不同加工方法的凉粉草进行质量评价,确定最佳采收时间和适宜的加工方法.方法:采用高效液相色谱法测定,色谱柱为Dikma Diamonsil(2)C18柱(250 mm×4.6 mm,5 μm);流动相为乙腈-2%甲酸(梯度洗脱);检测波长为320 nm;流速为0.8 ml·min-1;柱温为25℃.结果:凉粉草最佳采收月份为9～10月,最佳采收时间为午后16:00左右;加工方法可根据用途作相应的加工.结论:该法准确可靠,重复性好,为凉粉草药材生产质量控制提供了依据.