玻璃体切除术治疗急性视网膜坏死所致视网膜脱离

目的:观察玻璃体切除术治疗急性视网膜坏死所致视网膜脱离的临床效果。方法:回顾性分析2003-01/2008-01期间在我院行玻璃体切除术的急性视网膜坏死所致视网膜脱离患者15例(15眼),分析其视网膜脱离的特点,观察其临床治疗效果。结果:所有患者均行玻璃体切除术,联合行巩膜外环扎术10例,术中均行硅油填充术,术中视网膜出血2眼,术后并发性白内障4眼,高眼压1眼,低眼压1眼,取硅油后视网膜脱离复发1眼。术后6mo患眼最佳矫正视力除1眼放弃治疗外其余14眼均有不同程度地提高。结论:玻璃体切除术是治疗急性视网膜坏死综合征所致视网膜脱离的最佳治疗方法,对于部分病例,需联合行巩膜外环扎术。     

防风通圣散治疗荨麻疹(附2例报告)

防风通圣散是刘完素《宣明论方》中制定的方剂,是为表里三焦皆实而立。现在临床上多用此方治疗荨麻疹。经笔者临床验证,应用防风通圣散加减治疗荨麻疹确有很好的疗效。 例1,患者女,27岁。2年来,全身皮肤反复出疙瘩,时消时起,遇热加重,痒甚,伴咽干、口渴、心烦。检查:四肢躯干散发大小不等、形状不一的风团样扁平皮疹,肤色红,可见搔痕血痂,舌苔薄黄,舌质红,脉浮数。中医辨证:风热束表,发为风疹块。治则:疏     

去腐拔毒生肌膏治疗糖尿病合并小腿慢性溃疡

糖尿病合并小腿慢性溃疡是临床治疗中的难点,我院用自制去腐拔毒生肌膏治疗糖尿病合并小腿慢性溃疡16例,并取得了较为满意的效果,现报告如下。1 资料与方法 1.1 病例选择 本组16例中,女10例,男6例,年龄为50～68岁,平均57岁。16例均发生于小腿下段前外侧或前内侧,共有溃疡22处,最小1.5cm,最大4cm,平均为3cm。溃疡多深达肌层及骨膜外,溃疡均合并感染,有的结痂并痂下积脓,表浅的溃疡多有脓性分泌物,病程最短1个月,最长4年,其中1个月～1年者9例,1年以上者7例,平均15个月。 1.2 方药的组成 川乌、草乌、白芨、白芷、穿山甲、乳     

抗腮腺炎膏外敷治疗流行性腮腺炎1000例临床观察

流行性腮肠炎多发于儿童,以5～13岁儿童为多见,自1990年7月～1999年7月,在近万例临床病例中,我们对外敷抗腮腺炎膏的患者随机选择了1000例,并在外院设对照组300例,进行了临床观察,报告如下。     

病理性近视黄斑区的光学相干断层扫描图像分析

目的 研究分析病理性近视患者黄斑区的光学相干断层扫描(OCT)的图像特征.方法 对122例(238只眼)病理性近视患者行黄斑区OCT检查.患者屈光度数为-6.00～-32.00 D.结果 OCT检查黄斑区正常者11只眼(4.6％),黄斑区神经上皮层、视网膜色素上皮层和脉络膜毛细血管层光带反射不均匀及萎缩变薄者211只眼(88.7％);黄斑裂孔31只眼(13.0％),其中合并视网膜脱离者12只眼;无裂孔视网膜浅脱离11只眼(4.6％);黄斑部视网膜劈裂32只眼(13.4％),黄斑脉络膜新生血管28只眼(11.8％),黄斑前膜26只眼(10.9％),其中合并黄斑囊样水肿19只眼;Fuchs斑12只眼(5.0％),黄斑区色素沉着14只眼(5.9％),黄斑出血8只眼(3.4％).结论 OCT在观察病理性近视黄斑部病变具有独特的优越性,但也有一定的局限性.     

姜黄素对氧诱导的视网膜新生血管形成的影响

Objective To investigate the effects and mechanism of cureumin on the retinal neovasularization in mice with oxygen-induced retinopathy (OIR). Methods A total of 72 C57BL/6J mice were divided into normal, OIR model, vehicle control [dimethyl sulphoxide (DMSO)], and curcumin group(100, 50, and 10 mg). The mice in normal group lived in normoxia condition; OIR model was set up according to standard methods in the literature. Five days after OIR establishment, the mice in curcumin group received an intraperitoneal (IP) injection of 0.1 ml curcumin (100, 50, and 10 mg), and the mice in DMSO group received an IP injection of 0. 1 ml 1‰ DMSO. All of the mice were executed at the age of postnatal day 17 (P17) and the eyeballs were collected. Endothelial cell nuclei breaking through the internal limiting membrane were counted after stained with hematoxylin and eosin (HE). The expression of vascular endothelial growth factor-A (VEGF-A), vascular endothelial growth factor receptor-2 (VEGFR-2),endostatin (ES), and phosphorylated p38 mitogen-activated protein kinase (p-p38MAPK) in the retina in each group were measured by real-time polymerase chain reaction (RT-PCR) and Western blot methods.Results Compared with the normal group, retinal neovascularization was found in OIR model group (P<0. 05). The number of endothelial cell nuclei was 46.00 ± 16. 00 in OIR model group and 0. 17 ± 0.41 in normal group (P<0. 05). The expression of VEGF-A, ES, and p-p38MAPK in 100 nag curcumin group differed statistically from which in 50 and 10 mg curcumin group (P<0. 05). The expression of VEGFR-2 was same in the three curcumin groups (P>0. 05). Conclusion Curcumin can inhibit the formation of retinal neovascularization; the mechanism may be associated with inhibiting the expression of VEGFA and VEGFR-2, increasing the expression of ES, and inhibiting the p38MAPK signal transduction pathway.     

姜黄素对氧诱导的视网膜新生血管形成的影响

Objective To investigate the effects and mechanism of cureumin on the retinal neovasularization in mice with oxygen-induced retinopathy (OIR). Methods A total of 72 C57BL/6J mice were divided into normal, OIR model, vehicle control [dimethyl sulphoxide (DMSO)], and curcumin group(100, 50, and 10 mg). The mice in normal group lived in normoxia condition; OIR model was set up according to standard methods in the literature. Five days after OIR establishment, the mice in curcumin group received an intraperitoneal (IP) injection of 0.1 ml curcumin (100, 50, and 10 mg), and the mice in DMSO group received an IP injection of 0. 1 ml 1‰ DMSO. All of the mice were executed at the age of postnatal day 17 (P17) and the eyeballs were collected. Endothelial cell nuclei breaking through the internal limiting membrane were counted after stained with hematoxylin and eosin (HE). The expression of vascular endothelial growth factor-A (VEGF-A), vascular endothelial growth factor receptor-2 (VEGFR-2),endostatin (ES), and phosphorylated p38 mitogen-activated protein kinase (p-p38MAPK) in the retina in each group were measured by real-time polymerase chain reaction (RT-PCR) and Western blot methods.Results Compared with the normal group, retinal neovascularization was found in OIR model group (P<0. 05). The number of endothelial cell nuclei was 46.00 ± 16. 00 in OIR model group and 0. 17 ± 0.41 in normal group (P<0. 05). The expression of VEGF-A, ES, and p-p38MAPK in 100 nag curcumin group differed statistically from which in 50 and 10 mg curcumin group (P<0. 05). The expression of VEGFR-2 was same in the three curcumin groups (P>0. 05). Conclusion Curcumin can inhibit the formation of retinal neovascularization; the mechanism may be associated with inhibiting the expression of VEGFA and VEGFR-2, increasing the expression of ES, and inhibiting the p38MAPK signal transduction pathway.     

结肠镜检查前候诊时间对住院老年患者肠道准备质量的影响

目的探讨住院老年患者肠道准备质量的影响因素, 寻求老年患者肠道准备结束至开始检查的最佳候诊时间。方法收集同济大学附属第十人民医院2021年2—8月60岁以上需住院行结肠镜检查的老年患者资料。应用多因素分析筛选可能影响住院老年患者肠道准备质量的因素。按照候诊时间分组, 倾向性匹配分析后, 比较各组患者肠道准备质量差异。结果共有251例患者纳入研究。多因素分析显示, 高血压(OR=3.530, 95%CI 1.295～9.618)、慢性便秘(OR=3.302, 95%CI 1.132～9.632)、饮食依从性(OR=0.161, 95%CI 0.070～0.371)、服药及饮水量依从性(OR=0.167, 95%CI 0.070～0.397)、服药后活动量依从性(OR=2.245, 95%CI 1.040～4.845)、服药后排便次数(OR=0.446, 95%CI 0.308～0.647)、候诊时间(OR=0.537, 95%CI 0.387～0.745)是影响住院老年患者肠道准备质量的重要因素。不同候诊时间分组间的肠道准备质量存在差异, 120～180 min组肠道准备整体质量显著优于...     

姜黄素对氧诱导的视网膜新生血管形成的影响

目的 观察姜黄素对高氧诱导视网膜病变(OIR)动物模型新生血管形成的影响.方法 C57BL/6J小鼠72只,分为正常组、OIR模型组、二甲基亚砜(DMSO)组及100、50、10 mg姜黄素治疗组.正常组小鼠饲养于正常空气的氧浓度中,其余各组小鼠参照文献建立OIR模型.治疗组在建立OIR模型后分别腹腔注射100,50、10 mg/kg姜黄素0.1 ml,DMSO组腹腔注射1‰的DMSO 0.1 ml.所有小鼠于17日龄处死,摘除眼球,苏木精-伊红染色观察并计数突破视网膜内界膜的血管内皮细胞核的数目.取各组小鼠视网膜组织,逆转录-聚合酶链式反应(RT-PCR)及蛋白质免疫印迹(Western blot)方法检测血管内皮生长因子(VEGF)-A、VEGF受体-2(VEGFR-2)、内皮抑素(ES)、磷酸化p38丝裂原活化蛋白激酶(p-p38MAPK)在视网膜中的表达.结果 与正常组比较,OIR模型组小鼠视网膜有大量新生血管形成,突破内界膜的内皮细胞核数,OIR模型组为(46.00±16.00)个,正常组为(0.17±0.41)个,差异有统计学意义(P<0.05).RT-PCR及Western blot检测显示100 nag治疗组与其余两个剂量的治疗组相比在对VEGFA、ES、p-p38MAPR表达的影响上具有更为显著的作用(P<0.05),但不同剂量治疗组之间VEGFR-2的表达,差异无统计学意义(P>0.05).结论 姜黄素能够显著抑制氧诱导视网膜新生血管的生成.     

汉族人群Toll样受体3基因启动子区rs128912单核苷酸多态性与白内障的关系

目的：分析汉族人群Toll样受体3(TLR3)基因启动子区rs128912单核苷酸多态性(SNP)与白内障的关系。

方法：选取2019-06/2021-06我院收治的白内障患者263例作为研究组，晶状体脱位患者150例作为对照组。采用免疫印迹法(Western blotting)检测两组患者晶状体前囊膜组织中TLR3蛋白表达情况，采用直接测序法分析TLR3基因启动子区rs128912位点多态性，采用实时荧光定量-聚合酶链式反应(RT-qPCR)法检测不同基因型患者外周血TLR3 mRNA表达情况。

结果：研究组患者前囊膜组织中TLR3蛋白相对表达水平高于对照组(P<0.05)。研究组和对照组患者TLR3基因启动子区rs128912位点基因型(AA、AT、TT)频率均符合Hardy-Weinberg遗传平衡定律，且两组患者间TLR3基因启动子区rs128912位点基因型(AA、AT、TT)频率和等位基因(A、T)频率均有差异(P<0.05)。研究组中TT基因型患者外周血TLR3 mRNA相对表达水平均高于AA和AT基因型患者(P<0.05)。

结论：白内障患者晶状体前囊膜组织中TLR3蛋白表达明显上调，且TLR3基因启动子区rs128912位点多态性与汉族人群白内障易感性有关，携带TT基因型者更易发生白内障。