基层中医医疗机构患者对中医“治未病”的认知现状及影响因素分析

目的了解基层中医机构患者对中医治未病认知概况, 分析影响中医"治未病"的了解程度的因素, 为中医治未病在患者中的普及提供数据支持。方法 2022年5月至6月采用分层整群抽样法对广州市荔湾区、天河区、白云区、花都区在基层中医医疗机构2周内看过治未病科门诊或1年内定期接受治未病服务的630例患者(年龄20～60岁)进行调查。对基本情况做描述性分析, 影响因素分析使用χ2检验, 再使用logstic回归分析。结果 630例样本中有6.0%(38/630)认为非常了解中医"治未病", 24.3%(153/630)比较了解中医"治未病", 18.7%(118/630)和3.3%(21/630)不太了解和非常不了解中医"治未病", 47.1%(297/630)一般了解;影响患者接受中医"治未病"的因素包括服务内容、效果和支付费用, 影响患者对中医"治未病"的了解程度的影响因素有学历和职业。结论从卫生服务需求来看, 患者接受中医"治未病"以中医医院和社区卫生服务中心、乡镇卫生院为主, 服务形式以中医"治未病"门诊就诊和社区义诊咨询为主。     

Effect of Jin-3-needling therapy on plasma corticosteroid, adrenocorticotrophic hormone and platelet 5-HT levels in patients with generalized anxiety disorder

Objective:To observe the therapeutic efficacy of Jin-3-needling therapy(J3N)on generalized anxiety disorder(GAD)through clinical global impression scale(CGI), and to explore the mechanism by measuring the plasma levels of corticosteroid(CS),adrenocorticotrophic hormone (ACTH),and platelet 5-hydroxytryptamine(5-HT)before and after treatment.Methods:Eighty- six GAD patients with the diagnosis agreeing with the inclusion criteria were assigned,according to the sequence of visiting time,to three groups.The 29 patients in the Western medicine group were treated mainly with fluoxetine or paroxetine,Alprazolam might be given additionally in severe conditions if necessary;the 29 patients in the needling group received J3N therapy with Sishenzhen, Dingshenzhen,Neiguan(PC6),Shenmen(HT7)and Sanyinjiao(SP6)as the chief acupoints selected;and the 28 patients in the combined treatment group were treated with both drugs and needling in the same way as applied in the above two groups.The therapeutic course for all was 6 weeks.Conditions of patients were evaluated before and after treatment with CGI,and levels of CS, ACTH as well as 5-HT were measured by high performance liquid chromatography-electrochemistry. Results:By CGI scoring,the scores of severity index and the general index were not different significantly in the three groups,but the efficacy index proved to be the highest in the needling group, the second in the combined trentment group,and the lowest in the drug group.Plasma level of ACTH and platelet content of 5-HT were lowered in all the three groups after treatment,showing statistical significance(P<0.05),but no significant change was found in CS level(P>0.05).Conclusion:The therapeutic efficacy of J3N in treating GAD is equivalent to,but with the efficacy index significantly higher than,that of conventional treatment.Moreover,when combined with drugs,needling might effectively prevent the side effect of the routinely used Western drugs.The regulatory action of needling on platelet 5-HT and plasma ACTH is probably one of the acting pathways for J3N treatment on GAD.     

维氏气单胞菌TolC蛋白的生物信息学分析

目的 利用生物信息学技术预测和分析维氏气单胞菌TolC蛋白的生物学特性、结构、功能及进化关系,为研究其致病机制以及开发新的疫苗和新的药物靶点提供参考。方法 从NCBI蛋白质数据库中获取TolC序列,使用ProtParam、ProtScale、SignalP5.0、TMHMM2.0、PSORTb、NetPhos3.1、CD-search、SPOMA、SWISS-MODEL、ABCpred、IEDB等生物信息学工具分析和预测TolC蛋白的理化性质、亲疏水性、跨膜区域、信号肽、保守结构域、亚细胞定位、磷酸化位点、二级和三级结构、B细胞抗原表位和互作蛋白。利用MEGA5.2构建系统进化树,分析不同细菌间TolC蛋白的进化关系。结果 TolC蛋白由439个氨基酸组成,分子式为C₂₁₁₂H₃₄₁₄N₆₀₀O₆₈₉S₅,是稳定的亲水性蛋白。该蛋白亚细胞定位于细胞外,无跨膜结构域,有信号肽SP(Sec/SPI),含有52个磷酸化位点和多个B细胞抗原表位。蛋白的主要二级结构为α-螺旋,三级结构为...