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Seven female patients (mean age of 56 years) with advanced hip joint osteoarthritis underwent total hip replacement. Four days before operation they were given oral tetracycline for two days. During operation specimens were taken from the white articular cartilage, the yellowish articular cartilage and the ivory bone together with their subchondral bone tissues. The undecalcified specimens were cut into 10 μm sections and observed under the fluorescence microscope. In all the specimens the following findings could be observed. 1. The osteoarthritic articular cartilage became thinner, with uneven surface and fissures. 2. The superficial and deep surfaces and the central part of the subchondral bone plate showed bright golden yellow fluorescence. It reflected extensive new bone formation. 3. The subchondral bone trabeculae also revealed bright golden yellow fluorescence on their peripheral borders, so trabeculae turned thicker obviously. 4. The marrow tissues between the bone trabeculae exhibited particulate or spherical bright golden yellow fluorescence, reflecting new bone formation in the marrow. The particulate and spherical bright golden yellow fluorescent materials might aggregate, enlarge and merge into large piece of new bone and they also fused with the neighbouring bone trabeculae. The aforementioned changes in the structure of the subchondral bone tissues increased greatly the mass in the osteoarthritic femoral head. 相似文献
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成纤维细胞成骨潜能的体外培养研究 总被引:22,自引:0,他引:22
本研究采用新西兰种家兔皮肤的成纤维细胞进行体外培养,通过活体相关显微缩时录像,体视显微镜摄影,HE染色以及组织化学染色等技术做系列观察。发现成纤维细胞在体外培养条件下,不仅能产生粘多糖,胶原等基质成分,而且还能提供钙盐沉积。培养中期,成纤维细胞培养液显示A1P阳性反应;培养后期,培养液与成纤维细胞都可显示A1P阳性反应。 相似文献
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显示酸性及碱性磷酸酶的方法很多。但是经典的金属盐沉淀法由于受到多方面因素的影响可以出现假阴性、假阳性以及错误的定位,较理想的还是用偶氮法。然而偶氮法由于底质及重氮盐的不同,反应结果也不一致。萘酚AS磷酸酯是一种取代萘酚(substituted naphthols)。这种底质在合适的PH中经磷 相似文献
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低温塑料包埋切片技术是一种能应用于未脱钙骨制备切片标本的技术。由于采用低温包埋,从而使组织中的酶获的良好而持久的保存。Westen已较为全面地研究了这一技术,为骨组织酶组织化学研究提供了较为理想的方法使多种酯酶及氧化酶能获得持久保存。然而,Westen的技术只能应用于小块骨组织和骨髓活检标本。我们在家兔桡骨骨折模型应用这一技术,现就初步体会报告如下。 相似文献
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目的 探讨罗慕咬合诱导矫治器在儿童安氏II类错颌畸形早期矫治中的应用效果.方法 患儿入院后,进行头颅定位侧位X线全景影像收集,测量相关数据.完成后,佩戴罗慕咬合诱导矫治器,佩戴时间选择在晚18:00~早7:00.观察干预前和干预12个月后患儿骨骼变化情况、牙齿变化情况、鼻阻力、口鼻气流变化情况.结果 观察组患儿干预后ANB角、U1-SN角低于干预前和对照组,差异显著(P<0.05);观察组患儿干预后前牙覆(牙合)、前牙覆盖、下牙弓拥挤度低于干预前和对照组,下牙弓宽度、上牙弓宽度高于干预前和对照组,差异显著(P<0.05);观察组患儿干预后鼻阻力低于干预前和对照组,口气流低于干预前和对照组,鼻气流高于干预前和对照组,差异显著(P<0.05).结论 和戴用Activator干预方法 对比,罗慕咬合诱导矫治器可以较好的改善儿童安氏II类错颌畸形早期矫治效果,调整牙齿咬合状态. 相似文献
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