微波在磷钼酸铵容量法快速测定磷矿石中五氧化二磷的应用

在有柠檬酸的硝酸溶液中,微波作用下把溶矿和生成磷钼酸铵黄色沉淀一步完成;沉淀经过滤、洗涤后,溶于碱标准溶液中,然后用酸标准溶液滴定过量的碱,即可求出五氧化二磷的量,方法快速。     

比浊法测定磷矿石中微量氯

目前,磷矿石氯含量的分析方法尚无国家标准,常用的测定方法是离子选择电极法(0～700μg)[1],由于银电极较易损坏,给操作带来不便.比浊法虽灵敏度低(0～300μg)[2],但精密度好,方法简单,易于操控,且其他共存离子影响小.该方法用于磷矿石分析是一个简单、可行的方法.     

双侧植入人工耳蜗对儿童选择性听取能力影响的个案研究

目的探讨双侧人工耳蜗植入儿童在噪音环境下的选择性听取能力。方法采用实验法,研究个案在不同信噪比条件（SNR=0,10,20）下,单,双侧耳识别双音节词和短句的能力。测试使用计算机导航系统给声。结果在双音节词识别方面,当SNR=20和SNR=10时,单、双耳的识别率都为100％;但当SNR=0时,单、双耳识别率同时下降,结果较为接近。在短句识别方面,SNR=20和SNR=10时的结果相似,但单。双耳之间的差异较为明显;当SNR=0时,单、双耳识别率同时下降,结果较为接近。结论双侧人工耳蜗提高了该儿童在背景噪声中的选择性听取技能。SNR=20和SNR=10时,单耳和双耳的选择性听取能力都没有显著差异,但双耳明显好于单耳。在SNR=0时,单耳和双耳的选择性听取能力明显下降,且双耳和单耳差异缩小。     

大脑淀粉样血管病1例报告及文献复习

目的探讨大脑淀粉样血管病(CAA)的临床表现、诊断及治疗。方法回顾性分析1例CAA患者的临床资料,并结合相关文献对CAA的流行病学和病理学特点,临床和影像学表现,以及治疗和预后进行分析。结果 CAA的报道逐年增多,且发病率随年龄增加而增加;脑叶出血是CAA最常见的表现;该病诊断困难,确诊需病理学检查;对CAA所致的脑出血手术要慎重。结论目前还没有临床标准来区分脑出血是由CAA还是由其它原因所导致的。原发性、非外伤性和非高血压性老年患者脑出血应考虑CAA。     

HBV激活Notch1信号通路上调GP73的表达

目的:探索HBV上调GP73表达的相关机制.方法:通过real-time PCR和Western blot检测HepG2和整合HBV的HepG2.2.15细胞中Notch1及其下游分子Hes1的表达.分别转染Notch1过表达载体和干扰载体,观察Notch1对GP73表达的影响.免疫组织化学法检测GP73、Notch1在肝癌组织中的表达,分析两者的相关性.结果:HBV可引起Notch1的表达升高;Notch1可引起GP73的表达升高,两者在肝癌组织中呈正相关.结论:HBV可通过激活Notch1通路上调GP73的表达,这可能是GP73在肝癌组织中表达升高的原因之一.     

Expression of β-catenin protein in hepatocellular carcinoma and its relationship with alpha-fetoprotein

This study aimed to investigate the expression of β-catenin in hepatocellular carcinoma (HCC) tissues and its relationship with α-fetoprotein (AFP) in HCC. Immunohistochemistry was used to determine the expression of β-catenin in normal liver tissues (n=10), liver cirrhosis tissues (n=20), and primary HCC tissues (n=60). The relationship between β-catenin expression and clinical parameters of HCC was investigated. Real-time PCR and Western blotting were used to detect the mRNA and protein expression levels of β-catenin in the liver cancer cell line SMMC-7721 transfected with a plasmid encoding AFP, and also the mRNA and protein expression levels of β-catenin were measured in the liver cancer cell line Huh7 before and after the transfection with AFP shRNA plasmids. The results showed that β-catenin was only expressed on the cell membrane in normal liver tissues. Its localization to the cytoplasm and nucleus of cells was observed in a small proportion of cirrhotic tissues or adjacent HCC tissues, and such ectopic expression of β-catenin was predominant in HCC tissues. The abnormal expression of β-catenin was correlated with serum AFP levels, cancer cell differentiation and vascular invasion (P<0.05). Additionally, the increased expression of AFP resulted in the upregulation of β-catenin mRNA and protein levels, while knockdown of AFP with AFP shRNA led to significantly decreased β-catenin mRNA and protein levels (P<0.05). It was suggested that the abnormal expression of β-catenin is implicated in hepatic carcinogenesis and development. AFP can lead to increased expression of β-catenin, which may account for the poor prognosis of AFP-associated HCC patients.     

交流示波极谱滴定法测定萃取磷酸中的硫酸根

萃取磷酸由硫酸分解磷矿而得,生产中在线监控SO42含量至关重要。工业在线分析常用流动注射分析(F IA),实现快速、自动化测定。F IA分光光度法[1]、F IA热焓法[2]等均可用于测定SO42,但光度法选择性差,重现性难以保证;热焓法繁琐,费时,且不适于测定低含量SO42。文献[3]介绍了在N