1.6-氨基辛二酸-8-精-催产素对电针镇痛的影响

<正> 8-精-催产素(8-arginine-vasotocin,AVT)又名管催产素,是松果体分泌的九肽激素,其环状结构部分与催产素(oxytocin,OT)相同,直链部分与精氨酸加压素(Argi-     

mTOR/P70S6K信号通路活化在肝硬化门静脉高压症中意义的实验研究

目的 探讨mTOR/P70S6K信号通路在早期肝硬化门静脉高压症中的活化状态,证实mTOR信号通路的激活参与肝硬化门静脉高压症的发病,并探讨其可能机制.方法 本实验采用胆道结扎离断制备大鼠肝硬化门静脉高压症模型.雄性SD大鼠20只随机分为假手术组和模型组.通过组织病理学、形态学和血流动力学评估肝纤维化、炎症、和门静脉压力.采用RT-PCR和Western blot法分别测定肝纤维化标志物PC-αⅠ、α-SMA、TGFβ1及PDGF基因的转录和mTOR信号通路标志物P70S6K和磷酸化的P70S6K(P-P70S6K)的表达.结果 模型组大鼠胆道结扎离断3周后,P-P70S6K表达量明显增高而总蛋白P70S6K无显著差异,同时HSCs和胆管细胞活化增殖显著,肝脏促纤维化基因明显上调,间质胶原合成增加.结论 mTOR/P70S6K信号通路主要以磷酸化功能状态参与肝硬化门静脉高压症的形成,阻断该通路可能成为肝硬化门静脉高压症治疗的新靶向.     

链球菌感染相关的小儿自身免疫性神经精神障碍

儿童抽动障碍和强迫性障碍是基底节或基底节－丘脑－皮质回路病变所致的神经精神疾病，其病因不明。其中部分呈发作性经过的病例其发病或病情加重与链球菌感染后的自身免疫状态有关，被称为链球菌感染相关的小儿自身免疫性神经精神障碍。本文综述了这类疾患的现代概念以及有关的病因学研究、主要临床表现、诊断标准和治疗进展。     

蚯蚓溶栓酶制剂的致突变研究

蚯蚓溶栓酶制剂的致突变研究山西医学院（０３０００１）孙天佑，谭佩娇，刘志艳，白桂花蚯蚓溶栓酶制剂是由双胸蚓组织中提取制备的多组分酶蛋白，含有多种纤维蛋白溶解酶及纤溶酶原激活物，具有抗凝、促纤溶作用，改善血液流变学功效（降低全血、血浆粘度及血小板聚集性...     

神经元特异性烯醇化酶和癌胚抗原联合检测在肺癌与肺结核鉴别诊断中的价值

目的探讨神经元特异性烯醇化酶(NSE)和癌胚抗原(CEA)联合检测在肺癌与肺结核鉴别诊断中的价值。方法采用放射免疫法检测45例肺结核患者(肺结核组)和48例肺癌患者(肺癌组)NSE、CEA表达水平,同期选择本院45例门诊健康体检者作为对照组。比较不同病理类型肺癌患者CEA、NSE表达水平,观察NSE、CEA单一检测和两项联合检测诊断肺癌的敏感度和特异度。结果肺癌组患者血清NSE、CEA表达水平明显高于肺结核组和对照组(P0.05);腺癌患者血清CEA表达水平明显高于其他病理类型患者(P0.05),小细胞肺癌患者血清NSE表达水平明显高于其他病理类型患者(P0.05);两项指标联合检测诊断肺癌的敏感度(91.7%)均高于NSE和CEA单一诊断肺癌的敏感度(72.9%,66.7%)(P0.05)。结论血清NSE、CEA联合检测可作为肺癌与肺结核病鉴别诊断的重要实验室指标,还可有效提高诊断肺癌的敏感度。     

用胶片摄影法测定射线防护器材的铅当量

自从国务院发布第44号令以来,射线防护器材生产厂家及其制品发展很快。到目前为止全国大约有71家工厂生产33类110余种射线防护器材。这些厂家38%是乡镇企业,生产的射线防护器材规格、型式不一;并且许多生产单位缺乏必要的质量检测手段;所以致使一些射线防护器材存在着不同程度的质量问题。同时,我省目前大部分医疗机构的X射线防护器材仍然使用的是60年代或70年代的产品。存在不同程度的铅橡胶老化、或有裂隙、折断及铅脱落等质量问     

CYTOGENETIC STUDY ON THE PERIPHERAL LYMPHOCYTES OF PATIENTS WITH TROPHOBLASTIC TUMORS FOLLOWING CHEMOTHERAPY

Frequency distributions of sister chromatid exchange (SCE) and chromosomal breakage of the peripheral lymphocytes were studied in 25 patients during the treatment and 50 patients after recovery in order to know the effect of chemotherapeutic agents used for the treafment of chorioca.rcinoma and in.vasive mole. The 25 patients were observed consecutively for three courses. The results showed that there was no statistical difference between the patients with choriocarcinoma and invasive mole (p>0.05), and also no difference between different types of drugs used (p>0.05). However, SCE was higher after the first course'. The incidence of chromo- somal breakage after chemotherapy was twice as high as that before treatment. These indicate that during treatment, chemotherapeutic agents did induce some damage to the peripheral lym- phocytes. This damage, however, would only last for 2 years, because observation made on the 50 recovelred cas.es revealed a return to nor- mal in SCE and chromosoma.l breakage 2 years after recovery.     

血液透析患者动静脉内瘘口径及血流量的动态变化

血液透析是治疗终末期肾功能衰竭的主要手段之一 ,血液透析长期顺利地进行有赖于体外血液循环的有效建立 ,目前广泛应用于临床的建立血液循环的途径是动静脉内瘘 (arteriovenousfistula ,AVF) ,研究证明AVF造成动静脉直接交通 ,回心血量增加 ,增加心脏的前负荷 ,可导致高心输出性心力衰竭[1,2 ] ,然而这种影响是术后即刻形成 ?还是存在着动态变化 ,目前国内外文献尚未见报道。作者对血液透析患者AVF术后 3年吻合口径 (AVFD)及内瘘血流量 (AVFB)的动态变化进行了观察 ,现报告如下。1　对象与方法1.1　对象　　各种肾脏疾病导致的终末期慢性肾功能衰竭患者 34例     

Regulatory effects of herbal cake-partitioned moxibustion on the expressions of IL-17, IL-23 and their mRNAs in the colon of rats with Crohn’s disease

Objective

To observe the regulatory effects of herbal cake-partitioned moxibustion on Crohn's disease (CD) rat’s colon inflammatory cytokine interleukin-17 (IL-17), IL-23 and their mRNAs, and to investigate the action mechanism of moxibustion in treating CD.

Methods

Forty SPF grade Sprague-Dauley (SD) male rats were randomly divided into a normal group, a model group, an herbal cake-partitioned moxibustion group and a Western medicine group, with 10 rats in each group. Except the normal group, rats in the other three groups were used to make CD model by giving an enema in colon with Trinitro-benzene-sulfonic acid (TNBS). When the models were successful made, rats in the model group had no therapeutic intervention; rats in the herbal cake-partitioned moxibustion group were subjected to herbal cake-partitioned moxibustion treatment at bilateral Tianshu (ST 25) plus Qihai (CV 6); and rats in the Western medicine group were given oral Mesalazine. After treatment, the histopathological changes and inflammatory cytokines IL-17, IL-23 and their mRNAs expressions were observed in descending colon by hematoxylin-eosin (HE) staining, immunohistochemistry (IHC) and real-time polymerase chain reaction (RT-PCR) methods.

Results

Colon tissues of TNBS enema rat models showed cracks-like ulcers accompanied by mucous layer inflammation, granulomas, and inflammatory cytokines IL-17, IL-23 and their mRNAs expressions were all higher than those in the normal group (P<0.01); after intervention, colon tissue cracks-like ulcers and inflammation degree reduced, inflammatory cytokines IL-17, IL-23 and their mRNAs expressions were all significantly decreased (P<0.01) in the herbal cake-partitioned moxibustion group and the Western medicine group.

Conclusion

Herbal cake-partitioned moxibustion may improve colon tissue ulcers and relieve intestinal inflammation by down-regulation of IL-17, IL-23 and their mRNAs expressions in CD model rats’ colonic mucosa.