11221例围生儿出生缺陷监测结果分析

我院1996～2006年共出生围生儿11221例,其中出生缺陷儿92例,出生缺陷率为8.20‰,出生缺陷有33种。各系统畸形发生率主要有以下3种：神经系统、骨骼肌肉系统、泌尿系统。结果表明,农村出生缺陷率比城市高,缺陷儿比正常围生儿死亡率高,均具有非常显著性差异（P〈0.01）。只有坚决执行计划生育政策,做好婚前及围生期保健,重视产前诊断,选择性终止妊娠,才能有效提高人口素质。     

内源性乳糖结合凝集素在胃癌中的表达

目的：探讨内源性乳糖结合凝集素在胃癌中的表达及其意义。方法：采用亲和层析法分离纯化胃癌内源性乳糖结合颖集素,免疫家兔制备多克隆抗体,免疫组织化学方法（ＡＢＣ法）检测胃癌组织内源性乳糖结合凝集素表达。结果：纯化得到一分子量为３０ｋＤ的内源性乳糖结合凝集素,免疫组化检测发现胃癌乳糖结合凝集素表达阳笥率为３２．５％,Ⅰ、Ⅱ、Ⅲ期阳性率分别为２０．０％、２９．６％和５０．０％（Ｐ〉０．０５）,组织学高分子     

乳腺囊性增生病的诊治进展

乳腺囊性增生病的诊治进展杨得启，张嘉庆，乔新民乳腺囊性增生病于一１８４５年由英国人ＣＯｏｐｅｒ首先对本病进行描述。本病的命名在国内外文献中很混乱，自被称为囊性剥脱性上应增生、乳腺囊腺病、纤维囊性病、乳腺结构不良、乳腺小叶增生、乳腺腺病等。１９７２年全...     

IN VITRO TISSUE CUL,TURE STUDY ON GIANT CELL TUMOR OF BONE

In vitro tissue culture of 22 giant cell tumors (GCTs) of the bone in 19 cases was undertaken. Primary growth was successful in all. 11 were Grade I tumors, 10 Grade n and l Grade III. The average survival of the 3 grades of GCTs was in order 167.3, 219 and 625 days. Of these, 6 were postoperative recurrent GCTs, their life span ranging from 198 t0 6.25 days. It is apparent that the higher the pathologic grade of GCT, the longer the survival of cultured cells. Recurring tumors also manifested more active growth po- tential. This lends support to the view that the existing pathologic grading remains valuable for clinical reference. Multinucleated giant cell usually survived 3 weeks, occasionally 3 months. Growth of the stromal cells maint.ained from 5 weeks t0 8 months. The circumstantial evidence indicates that the stromal cells, not the giant cells, should be considered as the offending neoplastic invader.     

化疗药物敏感性测定方法近况

化疗是恶性肿瘤综合治疗主要措施之一,但并非所有化疗都能达到预期目的。相同组织类型,同样临床阶段的恶性肿瘤,对于不同的病人,即是用同一抗癌药物,同样治疗方案,其疗效常不一致。因为癌组织中癌细胞的异质性及不同肿瘤生物学特性的差异可影响肿瘤化疗敏感性,所以目前尚未发现某种抗癌药对所有的肿瘤都有效。据统计,凭临床经验选择药物进行肿瘤化疗的总有效率为14％,而大部分肿瘤病人化疗不但     

nm23—H1mRNA在人乳癌中表达与腋淋巴结转移的关系

目的:从分子水平探讨肿瘤转移及预后的影响因素.方法:应用逆转录聚合酶链反应(RT-PCR)定量技术,研究抗转移基因nm23-H_1mRNA在人乳癌中表达及其临床意义.结果:nm23-H_1高表达者占48.3%(29/60),其中腋淋巴无转移者为60.5%(23/38),而转移者为27.3%(6/22),二者间差异有显著性意义;mn23-H_1表达与其它临床参数关系不大.结论:nm23-H_1可能在抑制肿瘤转移过程中起一定的作用,其表达程度与乳癌淋巴结转移呈负相关.     

CELLULAR ELEMENTS IN GIANT CELL TUMOR OF BONE FEULGEN-DNA QUANTITATION, 3H_TdR INCORPORATION AND ULTRASTRUCTURE OBSERVATION

Anti-erythrocyte rosette assay was used to sub divide stromal cells (StCs) of cultured giant cell tumor (GCT) of bone into two groups, the rosette forming cells (RFCs) and non-rosette forming cells (NRFCs). Characteristics of Feulgen-DNA content, 3H.TdR uptake and ultrastructure of different cellular elemcnts in GCT were then investigated by micro- spectrophotometry, autoradiography and both TEM and SEM. Two types of StCs and multinucleated giant cells (MGCs) showed 2C DNA stem line, which contrasted strikingly with aneuploid DNA in osteosarcoma cells. Autoradiography revealed that the labelling index of NRFCs increased with the time of 3H.TdR incorporation in vitro, while RFCs and MGCs were scarcely tagged. The two types of StCs were distinctly different in both surface and intra- cellular structures. The phagocytic function and sur- face appearance of RFCs resembled those of ma crophages, and no polymorphism was found in RFCs. These facts suggest that NRFCs are the neoplastic element in GCT, whereas RFCs are the end-stage cells and possibly the macrophages related to tumor immunity.