红景天苷对缺血性脑损伤大鼠的作用

目的 基于超高效液相色谱-电喷雾-串联质谱(UPLC-ESI-MS/MS)技术研究红景天苷(salidroside, Sal)的血脑屏障(blood brain barrier, BBB)穿透性,并通过网络药理学、分子对接技术和动物实验探讨Sal治疗缺血性脑卒中(ischemic stroke, IS)的作用靶点及机制。方法 利用UPLC-ESI-MS/MS技术研究Sal的BBB穿透性;运用多个数据库预测Sal的作用靶点和IS的疾病靶点,构建PPI网络并筛选核心靶点,进行GO和KEGG富集分析,并通过分子对接技术和动物实验进行验证。结果 Sal给药正常大鼠和MCAO大鼠后,血浆和脑组织中均检测到Sal原形和代谢物酪醇;网络药理学共筛选出Sal治疗IS的作用靶点191个,GO功能富集分析主要涉及蛋白水解、细胞迁移的正调控等生物过程;KEGG通路分析提示PI3K-Akt、MAPK、FOXO等信号通路在Sal治疗IS过程中发挥关键作用;分子对接结果显示,Sal与对接的核心靶点均有良好的结合能力;动物实验结果表明,Sal可以明显改善MCAO大鼠的神经功能损伤,明显增加缺血侧Nissl阳性细胞的...     

The neuroprotective effect of salidroside prophylactic administration on MCAO model rats北大核心CSCD

Aim To investigate the neuroprotective effect of prophylactic administration of salidroside (Sal) on MCAO rats. Methods A total of 52 SD adult male rats were randomly divided into sham operation group (Sham), model group (MCAO) and salidroside pre-administration group (MCAO + Sal). The dose of Sal was 50 mg·kg-1. MCAO model was administered by continuous intraperitoneal injection for 7 days, followed by the wire embolization 0.5 h after the 7th day of administration, and the materials were taken 24 h after anesthesia. TTC staining was used to detect the volume of cerebral infarction in rats, TUNEL staining was used to detect the number of nerve cell apoptosis, Western blot was used to detect the expression of Bax, Bcl-2, cleaved Caspase-3 and total Caspase-3 proteins, naphthol AS-D chloroacetate was used to detect the expression of neutrophils in brain tissues, RT-qPCR was used to detect the mRNA expression of IL-1β, IL-6 and TNF-α, and immunofluorescence staining was used to determine the expression of NeuN in brain tissues. Results Pre-administration of salidroside for seven days significantly reduced cerebral infarct volume and nerve cell apoptosis in MCAO rats, promoted the expression of Bcl-2 protein, inhibited the expression of Bax and cleaved Caspase-3 proteins, but it had no significant effect on total Caspase-3 protein, and it reduced the recruitment of neutrophils in ischemic brain tissues, decreased the mRNA expression of inflammatory factors IL-1β, IL-6, TNF-α, and up-regulated the expression of NeuN. Conclusions Pre-administration of salidroside for seven days can significantly reduce the volume of cerebral infarction in MCAO rats, inhibit nerve cell apoptosis, reduce inflammation and promote the expression of NeuN, then playing a neuroprotective role. © 2023 Publication Centre of Anhui Medical University. All rights reserved.