藏药白沙蒿挥发油超临界CO2萃取工艺优化及其抗氧化活性研究

目的 采用Box-Behnken响应面法优化白沙蒿挥发油超临界CO₂萃取的最佳萃取工艺,并考察萃取物的抗氧化活性。方法 以白沙蒿挥发油的萃取得率为响应值,通过单因素试验筛选出萃取时间、萃取温度和萃取压力3个主要因素,进行Box-Behnken中心组合试验设计,建立挥发油提取得率的二次回归方程,得到优化组合条件。通过测定挥发油对1,1-二苯基-2-三硝基苯肼（1,1-diphenyl-2-picrylhydrazyl,DPPH）和2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸[2,2'-azino-bis（3-ethylbenzothiazoline-6-sulfonic acid）,ABTS]自由基的清除能力评价其抗氧化活性。结果 白沙蒿挥发油的最佳萃取工艺为萃取时间55 min,萃取温度59℃,萃取压力32 MPa,在此条件下挥发油萃取得率为（1.99±0.087）%,约为传统的水蒸气蒸馏法（萃取得率为1.05%）的1.9倍。超临界CO₂萃取的白沙蒿挥发油DPPH和ABTS自由基的IC₅₀值分别为0.264和0.045 mg·mL^-1。结论 优化后的白沙蒿挥发油萃取工艺稳定可靠,萃取的挥发油具有良好自由基清除作用,有望成为一种新的天然抗氧化剂。     

改善肺动脉高压中肺血管重构治疗策略的研究进展

肺动脉高压(pulmonary hypertension, PH)是一种慢性、进行性、高死亡率的疾病。各类PH具有相同特征：肺动脉内皮细胞和平滑肌细胞的过度增殖、抗凋亡和炎症导致的肺小血管的渐进性增厚,引发肺血管的重构和肺血管阻力增加,最终导致右心室肥厚、心衰、死亡。目前治疗PH的药物(L型钙通道阻滞剂、磷酸二酯酶5抑制剂、鸟苷酸环化酶激活剂、内皮素受体拮抗剂、合成前列环素及其类似物)主要通过松弛肺血管降低肺动脉压,不能根本治愈患者。因此,研发有效改善甚至逆转肺血管重构的药物,是治疗PH的关键。近年来,围绕肺血管重构开展的研究主要有：新的小分子化合物的合成;成熟药物的改造;联合用药及剂型改造等;基于中医“肺胀”等理论,对中药制剂以及中药复方的药效评价;中药单体的研究;新靶点的研究。     

Effects of stilbenes on glucose and lipid metabolism in insulin resistant HepG2 cells北大核心CSCD

Aim To explore the effect of four stilbenes including rhaponticin, desoxyrhaponticin, rhapontigenin and resveratrol on glucose and lipid metabolism in insulin-resistant HepG2 cells induced by high glucose and high fat. Methods The model of insulin resistance was established by incubating HepG2 cells with a complex of glucose and oleic acid. MTT assay was used to detect cell viability. The intracellular triglyceride (TG) and glucose levels were measured by the kit method. The lipid production was observed by oil red O staining, and the cell morphology and uptake of 2-NBDG were observed by confocal microscope. The PPAR signaling pathway and PI3K/Akt insulin signaling pathway related proteins were determined by Western blot to evaluate the effect of stilbenes on glycolipid metabolism in IR-HepG2 cells. Results The complex containing 50 mmol • L-1 glucose and 0.5 mmol • L-1 oleic acid induced a successful insulin resistance model in HepG2 cells for 48 hours. Compared with the model group, all the four compounds could reduce the content of TG and lipid accumulation, and increase the ability of glucose uptake. These compounds could significantly inhibit the expression level of peroxisome proliferatorsactivated receptor (PPAR-) lipid metabolism-related proteins, and up-regulate the expression of phosphoinositide 3 -kinase (PI3 K) glycometabolism-related proteins, among which rhaponticin and resveratrol had the most significant effect. Conclusions The four stilbenes may alleviate insulin resistance in HepG2 cells by regulation of glucose and lipid metabolism disorder, especially rhaponticin and resveratrol. The mechanism may be related to the regulation of PPAR signaling pathway and the activation of PI3 K/AKT/mTORl pathway to reduce lipid production and promote glucose uptake. © 2023 Publication Centre of Anhui Medical University. All rights reserved.