金莲花总黄酮成分的含量测定

目的 建立金莲花总黄酮的含量测定方法,比较不同提取溶液和方法对总黄酮的影响。方法 分光光度法于波长500nm处测定总黄酮的含量。结果 总黄酮的含量分别为：甲醇提取液为7%（n=5）,平均加样回收率为101.4%,RSD=0.46%;水提取液为10.9%(n=5）,平均加样回收率为95.9%,RSD=0.51%;超声乙醇提取液为4.7%（n=5）,RSD=1.1%。结论 采用不同处理方法,样品中总黄酮的含量不同,以水提取液最高,甲醇提取液次之,超声提取法最低。     

穿孔伤性白内障伴眼内局限性感染的手术治疗

目的探讨穿孔伤性白内障伴有眼内局限性感染的治疗时机及治疗方案。方法回顾性分析10例10眼的致伤物、就诊时间、临床表现，确定手术及术后治疗方案。结果4眼前房有轻度炎症，10眼局部均有与角膜创口相对应处的虹膜后粘连，术中发现虹膜后表面有白色斑片状膜样物，晶状体前囊及前皮质呈颗粒状灰白色浑浊。涂片7眼见真菌孢子或菌丝，3眼细菌；培养2眼有镰刀杆菌生长。随访时间9月～2年，无复发。结论钝性物致伤后的穿孑L伤性白内障，应细致观察虹膜晶状体(尤其是伤道处)的表现，警惕感染的可能，应常规作涂片和培养，彻底清除可疑病灶。不宜一期植入人工晶状体。     

空气消毒器现场消毒效果评估

目的选择适合医院使用的室内动态空气消毒器。方法将空气消毒器安装于相应体积的房间内,在静态及动态下,于开机前及连续开机后不同时间进行空气细菌含量检测,根据消亡率判定消毒效果。结果静态时,各空气消毒器在开机0.5h后,室内自然菌消亡率均>90%,消毒合格;动态时也有较好的消毒效果。结论空气消毒器解决了室内动态空气消毒,但必须正确选择和合理使用才能达到良好地消毒效果。     

医疗器械酶洗应注意的问题

目的探讨酶清洗剂在医疗器械清洗消毒过程中应用的重要性和应注意的问题。方法对酶清洗剂的基本原理、使用方法、注意事项和如何选择酶清洗剂进行分析。结果医疗器械的清洗必须使用液态、清澈透明、无泡或少泡,均匀无杂质、具有漂洁功能、最适水温的要求、对水质无限制的多酶清洗剂。结论医疗器械的清洗采用优质多酶清洗剂,对保证消毒成功和控制交叉感染具有重要意义。     

Haigis公式系数优化对提高人工晶状体度数预测准确性研究

目的 比较采用Haigis公式优化和非优化系数计算白内障手术植入人工晶状体术前预测与术后实际屈光度的差别.方法 诊断为年龄相关性白内障,取22 mm≤眼轴≤24 mm患者81例(127只眼),IOL Master测量眼部参数,使用不同系数Haigis公式对植入相应人工晶状体进行术前屈光度预测,根据不同系数分为优化和未优...     

人工晶状体眼近视力矫正对近立体视的影响

目的 探讨超声乳化白内障吸除及人工晶状体植入术后近立体视改善的方法和手段,为临床改善人工晶状体眼者生活质量提供依据.方法 前瞻性系列病例研究.随机收录年龄相关性白内障患者46例(92只眼),平均年龄(61.3±5.5)岁.其中单眼人工晶状体眼24例(A组),双眼人工晶状体眼22例(B组).测量调节幅度、相对调节、近附加、近立体视.对被收录者人工晶状体眼的一般资料及近附加等计量资料进行t检验,对受检者近立体视分布数据采用X2检验,以P<0.05有统计学意义.结果 A、B组人工晶状体眼移近法单眼调节幅度分别为(2.05±1.12)和(2.35±1.30)D;双眼近附加分别为(2.08±0.37)和(2.13±0.46)D,单眼近附加分别为(2.20±0.42)和(2.01±0.39)D,各组内双眼近附加与单眼近附加差异均有统计学意义(t=2.33,2.20;P<0.05),左右眼单眼近附加差异均有统计学意义(t=3.70,5.02;P<0.01).A、B组配戴单眼近附加时的近立体视优于双眼近附加,差异均有统计学意义(X2=6.46,7.02;P<0.05).结论 单眼近附加作为人工晶状体眼的近附加可以有效改善近屯体视.(中华眼科杂志,2008,44:711-714)     

Significant improvement of procedural safety in stenting for basilar stenosis: A historically controlled study

The basilar artery has the most perioperative complications in stenting compared to the other intracranial arteries. We aim to study whether the procedural safety in stenting for basilar stenosis has improved. This study was a single-arm, non-randomized trial that included historically controlled patients for comparison. Between January 2012 and March 2019, 147 consecutive patients with symptomatic basilar stenoses receiving elective stenting treatment were included in current basilar artery stenting (BAS) group. The prospectively collected and registered 120 patients by the same interventional team from September 2001 to November 2011 were set as historical BAS group for control. A total of 267 individuals were included in this study, with a mean age of 59.5 ± 8.1 years. The proportion of patients with lesion length >15 mm was 26.5% (39/147) in the current BAS group versus 4.2% (5/120) in the historical BAS group. We found significant differences between these 2 groups in Mori A (17.7% vs 42.5%) and Mori C patients (42.9% vs 13.3%). The proportion of patients receiving preoperative high-resolution magnetic resonance (HRMRI) evaluation was 83.0% (122/147) in the current BAS group versus 20.8% (25/120) in the historical group (P < .05). Balloon-expendable stent (BES) (n = 1), Wingspan (n = 34), and Enterprise (n = 112) stents were placed in the current BAS group. In contrast, only balloon-expendable stent (BES) (n = 48) and Wingspan (n = 72) were deployed in the historical BAS group. The incidence of the safety endpoint (SE) was 4.1% (involving 6 patients) in the current BAS group versus 11.7% (involving 14 patients) in the historical BAS group (P < .05). In multivariate analysis, no risk factor was associated with the occurrence of the safety endpoint (SE). When BAS cases operated by the surgical team accumulated to 120 to 150, the incidence of complications decreased significantly. This is the largest sample size study to discuss the safety of BAS. The significantly decreased incidence of complications indicates that the improving technical measures and the accumulation of operation experience are necessary.     

Incidence of posterior vitreous detachment after congenital cataract surgery: an ultrasound evaluation

Graefe's Archive for Clinical and Experimental Ophthalmology - To investigate the incidence of developing posterior vitreous detachment (PVD) in children after congenital cataract surgery. This...     

热断层成像技术用于乳腺癌裸鼠模型实验初探

目的探讨热断层成像技术(TTM)应用于乳腺癌裸鼠模型扫描的可行性。方法采用既往实验中用逆转录病毒介导RNA干扰的方法,已构建的能不同程度抑制人的乳腺癌细胞MCF7中LRP16基因表达的两株细胞pL374/MCF7(抑制率90%)和对照细胞系pGFPi/MCF7(阴性);选取BALB/c雌性裸小鼠共20只,随机分为两组,10只/组。分别接种pL374/MCF7及对照细胞系pLGFPi/MCF7。接种细胞6周后,用TTM观察裸鼠成瘤情况。将20只裸鼠处死,解剖观察,取形成的小结节和相应的肺组织进行HE染色。结果TTM值异常升高的裸鼠有13只。11只裸鼠有≤3mm小结节。HE结果显示,以上11只裸鼠体内形成的小结节为阳性肿瘤组织,4只肺部组织显示有阳性肿瘤细胞。TTM值异常升高与HE染色阳性高度相关。结论TTM技术可能在肿瘤的动物模型研究中发挥一定的作用。     

不同时机行椎管内阻滞分娩镇痛对镇痛效果及分娩结局的影响

目的 探讨不同时机行椎管内阻滞分娩镇痛对镇痛效果及分娩结局的影响.方法 收集2018年1月至2018年12月期间福建省泉州德诚医院收治临产后要求分娩镇痛产妇252例,随机分为对照组、实验组各126例,对照组在宫口开大3～4 cm时实施镇痛,实验组在宫口开大1～2 cm时实施镇痛,比较两组的镇痛效果及分娩结局.结果 实验...