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2.
Berlier W Bourlet T Lawrence P Hamzeh H Lambert C Genin C Verrier B Dieu-Nosjean MC Pozzetto B Delézay O 《Journal of medical virology》2005,77(4):465-474
X4 and R5 HIV strains are present in the semen of men infected with HIV but R5 isolates are transmitted preferentially. The role of human epithelial cells in this selection is addressed. Three human cervical cell lines-CaSki, SiHa, and HEC1A-and normal human vaginal cells from HIV-negative donors were characterized for HIV receptor expression and incubated with X4 and R5 laboratory-adapted strains or primary isolates. The infection was assessed by detection of intracellular HIV DNA. The three cell lines were shown to express on their surface the CXCR4 and GalCer molecules, but not the CD4 and CCR5 ones. The three cell lines and normal human vaginal cells were found to be selectively permissive to X4 HIV entry; the preincubation of the cell lines with rhSDF-1 inhibited this infection. The detection of the intracellular proviral DNA in the cell lines and in normal human vaginal cells demonstrated a selective integration of X4 strains. Additional experiments showed that no extracellular RNA was detected in the supernatants of HEC1A cells infected by X4 isolates either after 18 days of culture or after incubation with PHA-stimulated PBMCs and that no transmission occurred after co-culture between infected HEC1A cells and PHA-stimulated PBMCs. These results suggest specific sequestration of X4 strains by genital epithelial cells, which could explain, at least in part, the HIV tropism selection process during sexual intercourse. 相似文献
3.
Serum C-reactive protein concentrations in patients with pulmonary sarcoidosis. 总被引:1,自引:0,他引:1 下载免费PDF全文
In a prospective study serum C-reactive protein concentrations were measured in nine patients with "active" pulmonary sarcoidosis (as assessed by bronchoalveolar lavage lymphocyte counts, gallium-67 lung scanning, and serial pulmonary function testing), and in five patients with "inactive" disease. Active pulmonary sarcoidosis was associated either with no rise or with only a modest rise in serum C-reactive protein concentrations. In contrast, serum C-reactive protein concentrations in 12 patients with sputum positive pulmonary tuberculosis were considerably raised. Serum C-reactive protein may thus provide a valuable test in the differentiation of sarcoidosis from conditions which it may mimic and which are known to induce an acute phase response. 相似文献
4.
Jack CI Cottier B Jackson MJ Cassapi L Fraser WD Hind CR 《International journal of radiation oncology, biology, physics》1996,34(1):149-154
PURPOSE: Radiation pneumonitis is thought to occur as the result of excess free radical generation following radiotherapy. Various in vitro studies have shown that large doses of irradiation can cause membrane lipid peroxidation and the oxidation of protein sulphuryl groups. We, therefore, studied two circulating markers of lipid peroxidation and an indicator of "catalytic iron" (potentially available iron to catalyze the generation of free radicals) in patients undergoing radiotherapy. METHODS AND MATERIALS: The 9,11 diene conjugate of 9,12 linoleic acid, expressed as their molar ratio (percentage molar ratio (MR)) and thiobarbituric acid reactive acid-substances (TBARS), as well as levels of circulating desferrioxamine-chelatable iron assay, were assayed. Serial blood samples were taken over a 3-month period in 25 patients with inoperable nonsmall cell lung cancer. RESULTS: Ten patients developed radiation pneumonitis. The patients who developed pneumonitis showed a tendency for the serum percentage molar ratio to increase after a week. The change in the percentage molar ratio between Time 0 and 1 week of radiotherapy was significantly higher in the group that subsequently developed pneumonitis compared to the group that did not (p = 0.002). The initial serum TBARS levels in patients were not significantly elevated compared to controls and there was no difference in the serum TBARS levels in the pneumonitis and nonpneumonitis groups throughout the study period. After 1 week of radiotherapy the group that subsequently developed pneumonitis had a significantly higher level of desferrioxamine-chelatable iron (DFx-iron) compared with the nonpneumonitis group (p = 0.05). CONCLUSION: These data suggest that both the percentage MR and DFx-iron appear to reflect an increased susceptibility to develop radiation pneumonitis and after 1 week of radiotherapy they indicate patients who are likely to subsequently develop pneumonitis. Hence, these indicators could indicate the group of patients that could benefit from intervention therapies with antioxidants. 相似文献
5.
Hind A. M. Noureldin Ali M. Abdel-Aziz Mokhtar M. Mabrouk Amira H. K. Saad Ibrahim H. A. Badr 《RSC advances》2023,13(2):844
A simple, cost-effective, and efficient differential pulse voltammetric (DPV) assay for monitoring spiramycin adipate (SPA) in its dosage forms, urine, and milk samples at an activated glassy carbon electrode (GCE) was developed. GCE was electrochemically activated by anodization at a high positive voltage (2.5 V). The activated glassy carbon electrode (AGCE) was surface characterized, optimized, and utilized for the electrochemical assay of SPA. The electrochemical behavior of the AGCEs was investigated using cyclic voltammetry (CV) which shows a remarkable increase in the anodic peak of SPA in comparison with GCE. This behavior reflects a remarkable increase in the electrocatalytic oxidation of SPA at AGCE. The impacts of various parameters such as scan rate, accumulation time, and pH were investigated. The analytical performance of the activated glassy carbon electrodes was studied utilizing DPV. Under optimum conditions, the oxidation peak current exhibited two linear ranges of 80 nm to 0.8 μM and 0.85–300 μM with a lower limit of detection (LOD) of 20 nM. The developed assay exhibited high sensitivity, excellent repeatability, and good selectivity. Additionally, the developed SPA-sensitive modified GCE was successfully applied for SPA assay in its pharmaceutical dosage form and diluted biological fluids as well, with satisfactory recovery results which correlated well with the results obtained using spectrophotometry.An electrochemically activated glassy carbon electrode was utilized as a cost-effective, environmentally friendly, and operationally simple sensor for sensitive and selective determination of spiramycin in dosage form, spiked milk and urine samples. 相似文献
6.
Remodeling reflects the structural and functional deterioration that occurs in heart failure. Indices of remodeling constitute
an important marker of the severity of heart failure, and reverse remodeling is an accepted goal in the treatment of heart
failure. Cardiac resynchronization therapy (CRT) has been shown to reverse the remodeling process by improving ventricular
size, shape, and mass and reducing mitral regurgitation in the short and long term. Diastolic function, right ventricular
size, and atria exhibit reverse remodeling. Trials of medical therapy for heart failure strongly link remodeling indices with
outcomes, and emerging data suggest that remodeling indices may be among the most accurate predictors of long-term morbidity
and mortality in heart failure patients with CRT devices. This review discusses remodeling and focuses on the evidence for
CRT-induced reverse remodeling. 相似文献
7.
Michele Pighi Pascal Thériault-Lauzier Hind Alosaimi Marco Spaziano Giuseppe Martucci Tian-Yuan Xiong Jean Buithieu Luiz Fernando Ybarra Jonathan Afilalo Jonathon Leipsic Ozge Ozden Tok Negareh Mousavi Andrea Mangiameli Thomas Pilgrim Fabien Praz Stephan Windecker Nicolo Piazza 《JACC: Cardiovascular Interventions》2018,11(16):1614-1625
Performing transcatheter tricuspid valve interventions requires a thorough knowledge of right-heart imaging. Integration of chamber views across the spectrum of imaging modalities (i.e., multislice computed tomography, fluoroscopy, and echocardiography) can facilitate transcatheter interventions on the right heart. Optimal fluoroscopic viewing angles for guiding interventional procedures can be obtained using pre-procedural multislice computed tomography scans. The present paper describes fluoroscopic viewing angles necessary to appreciate right-heart chamber anatomy and their relationship to echocardiography using multislice computed tomography. 相似文献
8.
9.
Simple and sensitive normal and reverse flow injection methods for spectrophotometric determination of thiamine hydrochloride (THC) at the microgram level were proposed and optimized. Both methods are based on the reaction between THC and diazotized metoclopramide in alkaline medium. Beer’s law was obeyed over the range of 10–300 and 2–90 μg/mL, the limits of detection were 2.118 and 0.839 μg/mL and the sampling rates were 80 and 95 injections per hour for normal and reverse flow injection methods respectively. The application of both methods to commercially available pharmaceuticals produced acceptable results. The flow system is suitable for application in quality control processes. 相似文献
10.
Detection of pulmonary arteriovenous fistulae in patient with cirrhosis by contrast 2D echocardiography. 总被引:1,自引:0,他引:1 下载免费PDF全文
A case of multiple pulmonary arteriovenous fistulae associated with cirrhosis of the liver is described. The diagnosis was made by contrast two-dimensional echocardiography and confirmed by cardiac catheterisation and pulmonary angiography. As it is a sensitive and non-invasive technique, contrast two-dimensional echocardiography may be a useful screening test in cyanotic patients with liver disease in whom the presence of pulmonary fistulae is suspected. 相似文献