口服盐酸溴己新对干眼症患者的短期效果分析

目的 探究口服盐酸溴己新片对干眼症患者眼部症状及炎性反应的改善作用.设计 前瞻性病例系列.研究对象 2018年6-12月在东南大学附属中大医院江北院区眼科接受治疗的120例不同类型干眼症患者.方法 采用随机数字表法将其分为观察组和对照组,每组60例,对照组采用玻璃酸钠滴眼液进行治疗,观察组在对照组基础上联合盐酸溴己新片...     

Effect of polydatin on lens epithelial cell injury in rats with cataract北大核心

Objective To investigate the effect of polydatin on lens epithelial cell (LEC) injury in rats with cataract. Methods A total of 50 newborn rats were randomly divided into the control group, model group, low-dose polydatin group, high-dose polydatin group, and activator group, with 10 rats in each group. Except for the control group, rats in the rest groups were injected with sodium selenite subcutaneously to establish the cataract rat models. Since the second day, rats in the low- and high-dose polydatin groups were given eye drops containing 1.3 g·L-1 and 5.0 g·L-1 polydatin, respectively; in addition to 5.0 g·L-1 polydatin eye drops, rats in the activator group were also given the SRI-011381 hydrochloride (10 mg·kg-1) intragastrically; rats in the control and model groups were given normal saline in eyes. The lens opacity was examined by a slit lamp. Hematoxylin-eosin (HE) staining was performed to monitor the pathological changes in the lens. The total superoxide dismutase (SOD) and malondialdehyde (MDA) levels in LECs were measured. The LEC apoptosis was detected by flow cytometry. The expression levels of transforming growth factor-β (TGF-β), Smad2, Smad3, α smooth muscle actin (α-SMA), B-cell lymphoma-2 (Bcl-2), and Bcl-2-associated X (Bax) in LECs were measured by Western blot. Results The anterior capsule of rats in the control group was structurally clear, and LECs and lens fiber cells were closely arranged. Compared with the control group, the model group had structural disorder in the anterior capsule, degeneration and swelling in lens fiber cells, loose LEC arrangement, and vacuolization near the posterior capsule; besides, the lens opacity grade, the LEC apoptosis, and the expression levels of MDA, TGF-β, Smad2, Smad3, α-SMA, and Bax in the model group increased greatly (all P<0.05), and the expression levels of SOD and Bcl-2 decreased greatly (both P<0.05). Compared with the model group, the lens structure of rats in the low- and high-dose polydatin groups improved significantly, the lens opacity grade, the LEC apoptosis, and the expression levels of MDA, TGF-β, Smad2, Smad3, α-SMA, and Bax decreased greatly (all P<0.05), and the expression levels of SOD and Bcl-2 increased greatly (both P<0.05). Compared with the high-dose polydatin group, the lens structure damage in the activator group was aggravated, the lens opacity grade, the LEC apoptosis, and the expression levels of MDA, TGF-β, Smad2, Smad3, α-SMA, and Bax increased greatly (all P<0.05), and the expression levels of SOD and Bcl-2 decreased greatly (both P<0.05). Conclusion Polydatin can inhibit the apoptosis and oxidative stress of LECs in rats with cataract by regulating the TGF-β/Smads signal pathway, which in turn alleviates the LEC injury. © The Author(s) 2023.