免疫调节剂对小鼠实验性单纯疱疹性角膜炎发病的影响

实验组小鼠腹腔分别注射免疫调节剂胸腺五肽(TP5)或环孢霉素(CsA),对照组注射生理盐水(NS),尔后角膜感染单纯疱疹病毒(HSV),造成小鼠实验性单纯疱疹性角膜炎模型。用裂隙灯显微镜观察小鼠角膜上皮、角膜实质、角膜新生血管、结膜和眼睑的病变变化情况。结果:种毒唇4～6天,TP5组角膜上皮和角膜实质病变比NS组严重,差异有显著性,而CsA无此作用。三组小鼠新生血管形成程度差异无显著性。且均在第8天出现高峰。TP5组和CsA组的结膜和眼睑病变,比NS组严重。因此,在临床上,应根据不同病种和不同情况,慎重使用免疫调节剂。     

杞菊滴眼液治疗实验性干眼症泪液减少和角膜上皮病变

目的观察杞菊滴眼液对干眼症家兔泪液分泌,及干眼症大鼠角膜修复的影响。方法泪腺注射阿托品制作干眼症家兔模型,将20只实验家兔分为溶剂对照组、卡波姆滴眼液组、杞菊滴眼液低、中、高剂量组,分别予双眼点杞菊滴眼液的溶剂、0.2%卡波姆滴眼液、杞菊滴眼液（每日2次、4次、8次）,测定造模前、第1次泪腺注射阿托品后及治疗第3天、第7天时schirmer试验的值。摘除大鼠双侧泪腺,双眼点1%苯扎溴铵溶液制作角膜上皮损伤干眼症大鼠模型,将40只大鼠分为溶剂对照组、羧甲基纤维素钠滴眼液组、杞菊滴眼液低、中、高剂量组,羧甲基纤维素钠滴眼液组予双眼点0.5%羧甲基纤维素钠滴眼液,其余各组用药情况同家兔,记录治疗第7、14和21天角膜荧光素染色的评分值。结果家兔治疗第3天,卡波姆滴眼液组与溶剂对照组相比,schirmer试验值增加,差异有统计学意义（P〈0.05）,治疗第7天,杞菊滴眼液中、高剂量组与溶剂对照组相比,schirmer试验值增加,差异有统计学意义（P〈0.05）。大鼠治疗14天,杞菊滴眼液低、中、高剂量组的角膜荧光素染色评分低于溶剂对照组（P〈0.05）,治疗21天,杞菊滴眼液低、中、高剂量组和羧甲基纤维素钠滴眼液组的角膜荧光素染色评分均低于溶剂对照组（P〈0.05）。结论杞菊滴眼液可缓解实验性干眼症泪液减少,减轻角膜上皮损伤。     

L-肌肽对大鼠硒性白内障的影响

Objective To explore the effect of L-canosine in preventing and treating rat cataract induced by sodium selenite. Methods This was an experimental study. Cataract was induced in the rats by sodium selenite. Rats were divided into 3 groups and each group has 3 ones. L-canosine eye drops (50 g/L or 20 g/L) and 0.9% normal saline were respectively instilled to the rat eye for 3 weeks and examined. L-canosine was added to the medium of cultured rat cataract lens at 1.00, 0.10 and 0.01 g/L for 1 week and then examined. Cataract lens were studied by using two-dimensional eletrophoresis. Results One week after instillation of L-canosine, the scores of rat cataract were 2.22±0.65, 2.39±0.98 and 2.83±0.38 in 50 g/L, 20 g/L L-canosine and control groups, respectively. The lesion in 50 g/l, L-canosine group was lighter than that of the control group. There was significant difference between these two groups (P=0.013). On the 2nd and 3rd weeks, there was no difference among these three groups. After 1 week for culturing cataract lens, there was no difference between these three groups. The results of two-dimensional eletrophoresis showed that the protein number was 182 and 161 in the L-carnosine and control groups, respectively. High molecular weight protein decreased and low molecular weight protein increased in L-carnosine group. Conclusions L-carnosine at 50 g/L could restrain the development of early stage rat cataract. L-camosine could modulate the rat lens protein in vitro, but could not affect lens cataract scores.     

七种抗真菌药对眼部真菌的抗菌作用

目的分析治疗眼部真菌感染7种药物的有效性。设计对照实验研究。研究对象眼部分离76株真菌及7种抗真菌药物。方法用微量液基稀释法(M38P)测定特比萘芬(TBN)、两性霉素B(AmB)、酮康唑(KCZ)、咪康唑(MCZ)、伊曲康唑(ICZ)、氟康唑(FCZ)和5氟胞嘧啶(5FC)7种抗真菌药对眼部分离真菌的最低抑菌浓度(MIC)范围和平均值。主要指标药物最低抑菌浓度。结果TBN对眼部真菌作用最强,对镰刀菌和曲霉菌的MIC值和平均值最小,茄病镰刀菌的MIC为0.15～8μg/ml,平均值为1.10μg/ml。AmB对茄病镰刀菌作用仅次于TBN,其MIC为2.00～8.00μg/ml,平均值为3.97μg/ml。KCI、MCA和ICZ对茄病镰刀菌作用稍差,对其它菌种均有一定作用。5FC和FCZ(除黑曲霉和串珠镰刀各1例)最低抑菌浓度均>64μg/ml的最高浓度。结论TBNF对眼部真菌作用最强,AmB次之,KCZ、MCZ和ICZ对不同菌属均有作用,FCZ和5FC的作用较差。     

L-肌肽对大鼠硒性白内障的影响

Objective To explore the effect of L-canosine in preventing and treating rat cataract induced by sodium selenite. Methods This was an experimental study. Cataract was induced in the rats by sodium selenite. Rats were divided into 3 groups and each group has 3 ones. L-canosine eye drops (50 g/L or 20 g/L) and 0.9% normal saline were respectively instilled to the rat eye for 3 weeks and examined. L-canosine was added to the medium of cultured rat cataract lens at 1.00, 0.10 and 0.01 g/L for 1 week and then examined. Cataract lens were studied by using two-dimensional eletrophoresis. Results One week after instillation of L-canosine, the scores of rat cataract were 2.22±0.65, 2.39±0.98 and 2.83±0.38 in 50 g/L, 20 g/L L-canosine and control groups, respectively. The lesion in 50 g/l, L-canosine group was lighter than that of the control group. There was significant difference between these two groups (P=0.013). On the 2nd and 3rd weeks, there was no difference among these three groups. After 1 week for culturing cataract lens, there was no difference between these three groups. The results of two-dimensional eletrophoresis showed that the protein number was 182 and 161 in the L-carnosine and control groups, respectively. High molecular weight protein decreased and low molecular weight protein increased in L-carnosine group. Conclusions L-carnosine at 50 g/L could restrain the development of early stage rat cataract. L-camosine could modulate the rat lens protein in vitro, but could not affect lens cataract scores.     

L-肌肽对大鼠硒性白内障的影响

Objective To explore the effect of L-canosine in preventing and treating rat cataract induced by sodium selenite. Methods This was an experimental study. Cataract was induced in the rats by sodium selenite. Rats were divided into 3 groups and each group has 3 ones. L-canosine eye drops (50 g/L or 20 g/L) and 0.9% normal saline were respectively instilled to the rat eye for 3 weeks and examined. L-canosine was added to the medium of cultured rat cataract lens at 1.00, 0.10 and 0.01 g/L for 1 week and then examined. Cataract lens were studied by using two-dimensional eletrophoresis. Results One week after instillation of L-canosine, the scores of rat cataract were 2.22±0.65, 2.39±0.98 and 2.83±0.38 in 50 g/L, 20 g/L L-canosine and control groups, respectively. The lesion in 50 g/l, L-canosine group was lighter than that of the control group. There was significant difference between these two groups (P=0.013). On the 2nd and 3rd weeks, there was no difference among these three groups. After 1 week for culturing cataract lens, there was no difference between these three groups. The results of two-dimensional eletrophoresis showed that the protein number was 182 and 161 in the L-carnosine and control groups, respectively. High molecular weight protein decreased and low molecular weight protein increased in L-carnosine group. Conclusions L-carnosine at 50 g/L could restrain the development of early stage rat cataract. L-camosine could modulate the rat lens protein in vitro, but could not affect lens cataract scores.     

抗HSV单克隆抗体治疗兔单疱角膜炎的研究

应用5种抗HSV的McAb混合制剂对兔单疱角膜炎进行治疗实验。结果表明McAb有明显治疗作用,不但角膜病变程度轻,发展为角膜实质炎及虹膜炎的眼数也较少。两次实验结果相比说明McAb效价高,同时提高浓度改为眼膏剂型,每日治疗时间延长等均可提高治疗作用,与治疗HSK最有效的ACV无显著差异,是一种治疗HSK很有希望的制剂。     

L-肌肽对大鼠硒性白内障的影响

目的 研究L-肌肽防治大鼠硒性白内障的作用.方法 实验研究.用亚硒酸钠制作大鼠白内障模型,分为3组,每组9只,分别用50 g/L、20 g/L的L-肌肽滴眼液及生理盐水滴眼3周,观察大鼠晶状体变化情况.将大鼠白内障晶状体体外培养,分别加入1.00、0.10及0.01 g/L的L-肌肽无血清营养液,培养1周,观察大鼠晶状体变化.再将培养的大鼠白内障晶状体进行双向电泳实验.采用方差分析对各组间的均数进行比较.结果 大鼠用药1周,50g/L、20g/L的L-肌肽组和对照组的晶状体病变度数分别为2.22±0.65、2.39±0.98及2.83±0.38;50 g/L的L-肌肽组病变轻于对照组,差异有统计学意义(P=0.013).用药2周和3周,3组间晶状体病变差异无统计学意义(P>0.05).大鼠自内障晶状体培养1周,1.00、0.10及0.01 g/L的L-肌肽组晶状体病变与对照组差异无统计学意义(P>0.05).双向电泳结果显示:药物组蛋白总数为182,对照组为161,药物组高相对分子质量蛋白数量减少,低相对分子质量蛋白数量增多.结论 50 g/L的L-肌肽可抑制大鼠白内障早期病变的发展;肌肽在体外可使大鼠白内障品状体蛋白发生变化,对白内障晶状体病变程度作用不明显.     

拨云滴眼剂治疗家兔实验性金黄色葡萄球菌角膜炎

目的探讨拨云滴眼剂对家兔金黄色葡萄球菌角膜炎的治疗作用。设计实验研究。研究对象角膜感染金黄色葡萄球菌的家兔。方法将家兔角膜刮痕后接种金黄色葡萄球菌,分别用复方熊胆滴眼液、高、中、低浓度的拨云滴眼剂进行治疗,以拨云滴眼剂溶剂组和不给药组为对照。主要指标观察家兔结膜和角膜病变。结果结膜病变情况:复方熊胆滴眼液组、高、中、低浓度的拨云滴眼剂组与溶剂组无显著性差异;高浓度拨云滴眼剂组在第1、3、4和5天,中浓度拨云滴眼剂组存第1和4天,低浓度拨云滴眼剂组在第1、2和4天,与不给药组有显著性差异。角膜病变:高浓度拨云滴眼剂组在第1、3、4和5天,中浓度拨云滴眼剂组在第1、3、4、5和7天,低浓度拨云滴眼剂组在第3天,与溶剂组有显著性差异;复方熊胆滴眼液组在第1、4、5和6天与不给药组有显著性差异;高浓度拨云滴眼剂组在第1、3、4、5、6、7和8天,中浓度拨云滴眼剂组在第1、3、4、5、6、7、8和9天,低浓度拨云滴眼剂组在第5、6、7和8天,与不给药组有显著性差异。复方熊胆组、滴剂组、高浓度组、中浓度组、低浓度组和不给药组角膜培养的金黄色葡萄球菌阳性率分别为50.0%、33.3%、25.0%、16.7%、58.3%和50.0%。结论拨云滴眼剂治疗家兔金黄色葡萄球菌角膜炎有效。(眼科,2007,16:425-427)