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Cyclin A表达与人脑胶质瘤细胞增殖活性及其病理分级的关系 总被引:1,自引:1,他引:0
目的研究人脑胶质瘤中cyclin A表达与肿瘤细胞增殖活性及其病理分级的关系.方法采用免疫组化方法检测42例人脑胶质瘤标本中cyclin A和PCNA的表达水平,计算cyclin A阳性率和标记指数,以及PCNA阳性率和标记指数,分析它们与胶质瘤病理分级的关系.结果随着人脑胶质瘤病理分级的增高,cyclin A的阳性率和平均标记指数明显升高.在Ⅰ~Ⅳ级胶质瘤中其标记指数分别是(0.15±0.42),(1.59±1.95),(5.76±5.85)和(6.74±6.33);Ⅰ,Ⅱ级和Ⅲ,Ⅳ级间有显著性差异(P<0.01);其阳性率分别是11%,75%,71%和82%,在Ⅰ级与Ⅱ,Ⅲ,Ⅳ级间有显著性差异(P<0.05).PCNA标记指数随脑胶质瘤恶性程度提高而升高.Cyclin A与PCNA的表达呈正相关(r=0.858,P<0.01).结论人脑胶质瘤中cyclin A蛋白的阳性表达与细胞增殖活性和组织学分级密切相关,它可能参与了人脑胶质瘤细胞的恶性转化. 相似文献
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目的:探讨羟基磷灰石(hydroxyapatite,HA)义眼台植入在眼科整形美容手术中的临床应用。
方法:对52例52眼患者采用不同的手术方式行HA义眼台植入。A组:自体巩膜壳内Ⅰ期植入24例24眼;B组:双层巩膜帽覆盖义眼台肌锥内Ⅰ期植入16例16眼;C组:异体巩膜包裹义眼台Ⅱ期植入12例12眼。
结果:术后随访3~24mo,大部分患者植入义眼台后眼球饱满,并获得一定的眼球活动度(优71.2%;良19.2%;差9.6%)。
结论:HA是当前理想的眶内植入物,应用于临床可以收到满意的效果。 相似文献
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横向带蒂包皮皮管(Duckett术式)修补尿道下裂356例 总被引:1,自引:1,他引:0
目的:评价带蒂包皮皮管(Duckett术式)修补尿道下裂的疗效。方法:1995年3月至2010年12月共施行带蒂包皮皮管(Duckett术式)修复尿道下裂356例,其中年龄小于14岁的患儿324例(91.0%),修补尿道长度最长10 cm,最短1.5 cm。结果:发生尿道瘘共30例(8.4%),外口狭窄1例,吻合口狭窄1例,比较不同年龄段和不同时期手术并发症发生率无显著性差异(P>0.05),总成功率91.0%。结论:Duckett术式应是Ⅰ期治疗尿道下裂的首选方法,尤其适用于阴茎下屈需要横断并切除尿道板的患儿。 相似文献
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One of the critical steps in the progression to condyloma acuminatum (CA) is the establishment of a persistent human papillomavirus
(HPV) infection, majority of HPV type 6 and 11. Cytotoxic T lymphocytes (CTL), which can be induced by the epitope-based peptides
in vitro, are thought to be able to recognize and destroy virus-infected cells. In order to screen and identify HLA-A*0201
restricted HPV-11E7 CTL epitopes, five epitope peptides and tetramers were selected including HPV-11E7 7–15 (TLKDIVLDL), 15–23
(LQPPDPVGL), 47–55 (PLTQHYQIL), 81–89 (DLLLGTLNI) and 82–90 (LLLGTLNIV). Human monocyte-derived dendritic cells (DCs) from
HLA-A*0201 healthy individuals were pulsed with these peptides to assess the expression of CD83, CD86, HLA-DR and the secretion
of IL-12. The ability of peptide-loaded mature DCs to activate autologous T cells was evaluated by analyzing the frequency
of specific tetramer+ CD8+ T cells using flow cytometry, and the level of IFN-γ secretion by ELISA. The ability of the epitope-specific CTLs to kill
the target cells was also analysed. It was found that the immature DCs could be fully activated by all the five HPV-11E7 peptides
and peptide-loaded mature DCs were able to stimulate the epitope-specific T cells in vitro. There was an increased frequency
of CD8+ T cells specific for the E7 7–15 epitope when compared to other four predicted epitopes of HPV-11E7 (P < 0.05). The epitope-specific CTLs for E7 7–15 induced the strongest cytotoxicity to HPV-11E7 expressing cell line at an
E:T ratio of 50:1 (P < 0.05). Taken together, these findings demonstrate that E7 7–15 (TLKDIVLDL) is an HLA-A*0201-restricted CTL epitope of HPV
type 11. We propose that this epitope could be more helpful in the characterization of HPV control mechanism and be useful
for the development of immunotherapeutic approaches for low-risk HPV infectious diseases such as CA.
The abstract was accepted by 23rd HPV International Conference and by the American Academy of Dermatology Academy 2007. 相似文献
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目的 评价超声乳化人工晶状体植入术治疗剥脱综合征(exfoliation syndrome.ES)并发白内障的疗效.方法 对20例(26眼)ES并发白内障的患者实施超声乳化人工晶状体植入术,同时随机选择老年性白内障患者50例(62眼)行超声乳化人工晶状体植入术作为对照.所有患者术后随访6~12个月.结果 术后视力>0.5者,ES组有23眼(88.4%),对照组有56眼(90.3%).ES组术中未发生晶状体后囊膜破裂玻璃体溢出.对照组1眼(1.6%).2组比较差异无统计学意义(P>0.05).ES组中晶状体悬韧带断裂1眼(3.8%),对照组1眼(1.6%),2组比较差异无统计学意义(P>0.05).结论 超声乳化人工晶状体植入术是治疗ES并发白内障的安全有效的方法.[眼科新进展2009;29(6):464-465] 相似文献
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例 1,女 ,10岁。因发作性睡眠 4年 ,加重 1年入院。患儿于 4年前无诱因出现阵发性发困及睡眠 ,开始每周 1~ 2次 ,近 1年每日发作数次 ,每次持续数分至数 10分钟 ,在坐车、上课、吃饭等任何情况下均可发生不可抗拒睡眠 ,入睡时有幻觉 ,可唤醒 ,无意识障碍。在情绪激动 ,尤其是大笑时即感双下肢无力而不能站立 ,但无猝倒、睡瘫。患儿无特殊既往史及家族史。神经系统检查 :双侧踝阵挛阳性 ,皮肤划痕征阳性 ,余正常。头颅CT ,MRI正常。脑电图清醒闭目时为界限性脑电图 ,睁眼时有睡眠波。剥夺睡眠脑电图正常。血常规及血生化正常。入院后予… 相似文献