全文获取类型
收费全文 | 280篇 |
免费 | 12篇 |
专业分类
耳鼻咽喉 | 5篇 |
儿科学 | 10篇 |
基础医学 | 32篇 |
口腔科学 | 16篇 |
临床医学 | 24篇 |
内科学 | 74篇 |
皮肤病学 | 9篇 |
神经病学 | 8篇 |
特种医学 | 2篇 |
外科学 | 20篇 |
综合类 | 4篇 |
预防医学 | 42篇 |
眼科学 | 6篇 |
药学 | 26篇 |
中国医学 | 1篇 |
肿瘤学 | 13篇 |
出版年
2023年 | 1篇 |
2022年 | 2篇 |
2021年 | 5篇 |
2020年 | 1篇 |
2019年 | 3篇 |
2018年 | 12篇 |
2017年 | 5篇 |
2016年 | 8篇 |
2015年 | 9篇 |
2014年 | 3篇 |
2013年 | 18篇 |
2012年 | 33篇 |
2011年 | 21篇 |
2010年 | 17篇 |
2009年 | 4篇 |
2008年 | 27篇 |
2007年 | 21篇 |
2006年 | 20篇 |
2005年 | 19篇 |
2004年 | 15篇 |
2003年 | 15篇 |
2002年 | 20篇 |
2001年 | 1篇 |
1999年 | 3篇 |
1991年 | 1篇 |
1990年 | 1篇 |
1989年 | 2篇 |
1987年 | 5篇 |
排序方式: 共有292条查询结果,搜索用时 62 毫秒
1.
André Luís Conde Watanabe Mateus Silva Feijó Vinícius Paulo Lima de Menezes Mayara Regina Galdino-Vasconcelos Jorge Luis Salinas Caballero Gustavo Ferreira Fernando Jorge Natália Trevizoli Luiz Gustavo Diaz Priscila Brizolla de Campos Gabriel Cajá Raquel Ullmann Ana Virgínia Figueira Tiago Morato Adriano Moraes Juan Rafael Branez Pereira Marcelo Perosa 《Transplantation proceedings》2021,53(1):73-82
IntroductionLiver transplantation is the standard treatment for end-stage liver disease. Brazil holds the third highest number of liver transplants performed per year, but center maldistribution results in high discrepancies in accessing this treatment. In 2012, an interstate partnership successfully implemented a new liver transplantation program in the middle west of Brazil. Here, we report the results of the first 500 liver transplants performed in this new program and discuss the impacts of a new transplant center in regional transplantation dynamics.MethodsWe reviewed data from the first 500 consecutive deceased donor liver transplants performed in the new program during an 8-year period. We analyzed data on patients’ clinical and demographic profiles, postoperative outcomes, and graft and recipient survival rates. Univariate survival analysis was conducted using log-rank tests to compare the groups.ResultsAlmost half (48%) of the procured organs and 40% of the recipients transplanted in our center were from outside our state. Recipient 30-day mortality was 9%. Overall recipient survival at 1 year and 5 years was 85% and 80%, respectively. Mortality was significantly associated with higher Model for End-Stage Liver Disease (P < .001) but not with the presence of hepatocellular carcinoma (P = .795).DiscussionThe new transplantation program treated patients from different regions of Brazil and became the reference center in liver transplantation for the middle west region. Despite the recent implementation, our outcomes are comparable to experienced centers around the world. This model can inspire the creation of new transplantation programs aiming to democratize access to liver transplantation nationwide. 相似文献
2.
Virgília S Silva Ana I Duarte A Cristina Rego Catarina R Oliveira Paula P Gon?alves 《Toxicological sciences》2005,88(2):485-494
The ability of aluminum to inhibit the (Na(+)/K(+))ATPase activity has been observed by several investigators. The (Na(+)/K(+))ATPase is characterized by a complex molecular heterogeneity that results from the expression and differential association of multiple isoforms of both catalytic (alpha) and regulatory (beta) subunits. For instance, three main alpha (alpha(1), alpha(2) and alpha(3)) and three beta (beta(1), beta(2) and beta(3)) subunit isoforms exist in vertebrate nervous tissue, whereas only alpha(1) and beta(1) have been identified in kidney. However, no studies have focused on determining the change in (Na(+)/K(+))ATPase isoforms caused by chronic exposure to aluminum and its relation with aluminum toxicity. In this study, adult male Wistar rats were submitted to chronic dietary AlCl(3) exposure (0.03 g/day of AlCl(3) for 4 months), and the activity and protein expression of (Na(+)/K(+))ATPase isozymes were studied in brain cortex synaptosomes and in kidney homogenates. The intracellular levels of adenine nucleotides, plasma membrane integrity, and aluminum accumulation were also studied in brain synaptosomes. Aluminum accumulation upon chronic dietary AlCl(3) administration significantly decreased the (Na(+)/K(+))ATPase activity measured in the presence of nonlimiting Mg-ATP concentrations, without compromising protein expression of alpha-subunit isoforms in brain and kidney. Aluminum-induced synaptosomal (Na(+)/K(+))ATPase inhibition was due to a reduction in the activity of isozymes containing alpha(1)-alpha(2) and alpha(3)-subunits. The onset of enzyme inhibition was accompanied by a decrease of the (Na(+)/K(+))ATPase sensitivity to submicromolar concentrations of ouabain, and it preceded major damage in plasma membrane integrity and energy supply, as revealed by the analysis of lactate dehydrogenase leakage and endogenous adenine nucleotides. The data suggest that, during chronic dietary exposure to AlCl(3), brain (Na(+)/K(+))ATPase activity drops, even if no significant alterations of catalytic subunit protein expression, cellular energy depletion, and changes in cell membrane integrity are observed. Implications regarding underlying mechanisms of aluminum neurotoxicity are discussed. 相似文献
3.
Virgínia Carla de Oliveira Tatsuya Nagata Felipe C. Guimarães Fernanda A. Ferreira Elliot Watanabe Kitajima Cícero Nicolini Renato de Oliveira Resende Alice Kazuko Inoue-Nagata 《Virus genes》2013,46(1):190-194
A tymovirus was isolated in Brazil from tomato plants with severe symptoms of leaf mosaic and blistering. The virus was mechanically transmissible to solanaceous indicator host species. The infected plants contained icosahedral particles and chloroplasts with membrane deformations which are typical cytopathic effects caused by tymoviruses. Its coat protein amino acid sequence shares the maximum of 64 % identity with the tymovirus Chiltepin yellow mosaic virus, which suggested that it can be considered as a distinct member of the genus Tymovirus. In a phylogenetic tree, this tymovirus was clustered with other solanaceous-infecting tymoviruses. It was tentatively named as Tomato blistering mosaic virus (ToBMV). 相似文献
4.
5.
Juliana Bastos Costa Virna Lygia Lobo Rocha de Sousa Pedro Bezerra da Trindade Neto Thomás de Aquino Paulo Filho Virgínia Célia Dias Florêncio Cabral Patrícia Moura Rossiter Pinheiro 《Anais brasileiros de dermatologia》2012,87(6):910-913
Norwegian scabies is a highly contagious skin infestation caused by an ectoparasite,
Scarcoptes scabiei var. Hominis, which mainly affects immunosuppressed individuals.
Clinically, it may simulate various dermatoses such as psoriasis, Darier''s disease,
seborrheic dermatitis, among others. This is a case report of a 33-year-old woman,
immunocompetent, diagnosed with generalized anxiety disorder (cancer phobia), who had
erythematous, well-defined plaques, covered with rupioid crusts, on her neck,
axillary folds, breast, periumbilical region, groin area, besides upper back and
elbows, mimicking an extremely rare variant of psoriasis, denominated rupioid
psoriasis. 相似文献
6.
7.
Michele Stürmer Isadora M. Garcia Virgínia S. Souza Fernanda Visioli Jackson D. Scholten Susana M.W. Samuel Vicente C.B. Leitune Fabrício M. Collares 《Dental materials》2021,37(2):223-235
ObjectiveFormulate experimental adhesives containing titanium dioxide nanotubes (nt-TiO2) or titanium dioxide nanotubes with a triazine-methacrylate monomer (nt-TiO2:TAT) and evaluate the effect of these fillers on the physical, chemical, and biological properties of the adhesives.MethodsFirst, nt-TiO2 were synthesized via a hydrothermal method. The nt-TiO2 were mixed with a triazine-methacrylate monomer (TAT) to formulate nt-TiO2:TAT, which were characterized by transmission electron microscopy (TEM). The nt-TiO2, TAT, and nt-TiO2:TAT were evaluated via Fourier Transform Infrared, Ultraviolet–visible, and micro-Raman spectroscopies. An experimental adhesive resin was formulated with bisphenol A glycerolate dimethacrylates, 2-hydroxyethyl methacrylate, and photoinitiator/co-initiator system. nt-TiO2 or nt-TiO2:TAT were incorporated at 2.5 wt.% and 5 wt.% in the adhesive. The base resin without nt-TiO2 or nt-TiO2:TAT was used as a control group. The adhesives were evaluated for antibacterial activity, cytotoxicity, polymerization kinetics, degree of conversion (DC), Knoop hardness, softening in solvent (ΔKHN%), ultimate tensile strength (UTS), 24 h- and 1 year- microtensile bond strength (μ-TBS).ResultsTEM confirmed the nanotubular morphology of TiO2. FTIR, UV–vis, and micro-Raman analyses showed the characteristic peaks of each material, indicating the impregnation of TAT in the nt-TiO2. Adhesives with nt-TiO2:TAT showed antimicrobial activity against biofilm formation compared to control (p < 0.05), without differences in the viability of planktonic bacteria (p > 0.05). All groups showed high percentages of pulp cell viability. The polymerization kinetics varied among groups, but all presented DC above 50%. The addition of 5 wt.% of nt-TiO2 and both groups containing nt-TiO2:TAT showed higher values ??of Knoop hardness compared to the control (p < 0.05). The groups with nt-TiO2:TAT presented lower ΔKHN% (p < 0.05) and higher UTS (p < 0.05) than the control group. After one year, the group with 5 wt.% of nt-TiO2, as well as both groups containing nt-TiO2:TAT, showed higher μ-TBS than the control (p < 0.05).SignificanceThe mixing of a triazine-methacrylate monomer with the nt-TiO2 generated a filler that improved the physicochemical properties of the adhesive resins and provided antibacterial activity, which could assist in preventing carious lesions around tooth-resin interfaces. The set of physical, chemical, and biological properties of the formulated polymer, together with the greater stability of the bond strength over time, make nt-TiO2:TAT a promising filler for dental adhesive resins. 相似文献
8.
9.
Monalisa Cesarino Gomes Matheus França Perazzo Erick Tássio Neves Maria Betânia Lins Dantas Siqueira Edja Maria Melo de Brito Costa Ane Polline Lacerda Protasio Adriana Freitas Lins Pimentel Silva Kátia Virgínia Guerra Botelho Ana Flávia Granville-Garcia 《Zeitschrift fur Gesundheitswissenschaften》2018,26(5):587-594
10.
Patrícia Machado Rui Pereira Ana Mafalda Rocha Licínio Manco Natércia Fernandes Juliana Miranda Letícia Ribeiro Virgílio E. Do Rosário António Amorim Leonor Gusmão Ana Paula Arez 《British journal of haematology》2010,149(5):775-784
The genetic component of susceptibility to malaria is both complex and multigenic and the better‐known protective polymorphisms are those involving erythrocyte‐specific structural proteins and enzymes. In vivo and in vitro data have suggested that pyruvate kinase deficiency, which causes a nonspherocytic haemolytic anaemia, could be protective against malaria severity in humans, but this hypothesis remains to be tested. In the present study, we conducted a combined analysis of Short Tandem Repeats (STRs) and Single Nucleotide Polymorphisms (SNPs) in the pyruvate kinase‐encoding gene (PKLR) and adjacent regions (chromosome 1q21) to look for malaria selective signatures in two sub‐Saharan African populations from Angola and Mozambique, in several groups with different malaria infection outcome. A European population from Portugal, including a control and a pyruvate kinase‐deficient group, was used for comparison. Data from STR and SNP loci spread along the PKLR gene region showed a considerably higher differentiation between African and Portuguese populations than that usually found for neutral markers. In addition, a wider region showing strong linkage disequilibrium was found in an uncomplicated malaria group, and a haplotype was found to be associated with this clinical group. Altogether, this data suggests that malaria selective pressure is acting in this genomic region. 相似文献