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排序方式: 共有893条查询结果,搜索用时 31 毫秒
1.
The role of glycan moieties in thyrotropin receptor molecule in binding of antibodies is a subject of intense debate. To approach the function of sugars in recognition by antibody of the extracellular part of the receptor (ETSHR) we studied the reaction of the HPLC purified ETSHR from insect cells in the reaction with autoantibodies and antibodies of animal origin. None of the autoantibodies from Graves' patients sera bound to ETSHR. In contrast, each of the animal antibodies: three monoclonal, five polyclonal antireceptor and two polyclonal anti peptide corresponding to the amino acid sequence present in the receptor, became bound to the native receptor from insect cells as well as to its deglycosylated form. The shape of the dilution curves of particular antibodies in the reaction with either form of the receptor was almost the same. The coefficients of correlation was about 0.9. It seems that the correct receptor glycosylation is not crucial for binding of animal origin antibodies. 相似文献
2.
Urszula Krzych Robert Schwenk Mimi Guebre‐Xabier Peifang Sun Dupeh Palmer Katherine White Isaac Chalom 《Immunological reviews》2000,174(1):123-134
Summary: Exposure to irradiated Plasmodium sporozoites (g‐spz) results in protection against malaria. Like infectious spz, g‐spz colonize hepatocytes to undergo maturation. Disruption of liver stage development prevents the generation of protection, which appears, therefore, to depend on liver stage antigens. Although some mechanisms of protection have been identified, they do not include a role for intrahepatic mononuclear cells (IHMC). We demonstrated that P. berghei g‐spz‐immune murine IHMC adoptively transfer protection to naive recipients. Characterization of intrahepatic CD4+ T cells revealed an immediate, albeit transient, response to g‐spz, while the response of CD8+ T cells is delayed until acquisition of protection. It is presumed that activated CD8+ T cells home to the liver to die; g‐spz‐induced CD8+CD45RBloCD44hi T cells, however, persist in the liver, but not the spleen, during protracted protection. The association between CD8+CD45RBloCD44hi T cells and protection has been verified using MHC class I and CD1 knockout mice and mice with disrupted liver stage parasites. Based on kinetic studies, we propose that interferon‐g, presumably released by intrahepatic effector CD8+ T cells, mediates protection; the persistence of CD8+ T cells is, in turn, linked to Plasmodium antigen depots and cytokines released by CD4+ T cells and/or NK T cells. 相似文献
3.
Jan K. Lacki Kamilla Klama Stefan H. Mackiewicz Urszula Mackiewicz Wolfgang Müller 《Inflammation research》1995,44(1):24-26
In order to evaluate the relationship between serum concentrations of interleukin-10 (IL-10), IL-6, and acute phase proteins in rheumatoid arthritis (RA) patients treated with methotrexate (MTX) or intramuscular gold (IMG) we determined IL-10, IL-6, C-reactive protein (CRP), alpha-1-acid glycoprotein (AGP) and alpha-1-antichymotrypsin (ACT) in the sera of 35 RA patients. IL-10 and IL-6 levels were evaluated using an enzyme-linked immunoassay (ELISA). AGP and ACT level were measured using rocket immunoelectrophoresis. IL-10 serum level was not increased in RA patients as compared to controls (58.7 ± 18.1 pg/ml vs. 57.2 ± 11.9 pg/ml). IL-6 level was significantly elevated (91.6 ± 46.9 pg/ml vs. 45 ± 19 pg/ml, p < 0.05). CRP was significantly increased as compared to healthy controls (35 ± 19 mg/l vs. 3 ± 2 mg/l, p < 0.05). Patients treated with MTX or IMG presented an increased level of IL-10 and decreased amounts of IL-6, as compared to those treated with NSAID only. However, only changes between patients treated with IMG and NSAID were found to be statistically significant. A good negative correlation between IL-10 and IL-6 serum level was found (r = –0.75, p < 0.05). A positive significant correlation between IL-6 serum level and CRP (r = 0.62, p < 0.05), AGP (r = 0.78, p < 0.05) and ACT (r = 0.45, p < 0.05) was established. On the other hand, a negative correlation between IL-10 and serum level of CRP (r = –0.76, p < 0.05), AGP (r = –0.64, p < 0.05) and ACT (r = –0.38, p < 0.05) was also observed. Moreover, these relationships were maintained when patients treated with MTX, IMG, or NSAID were analyzed independently. According to the data thus far obtained, it seems that IL-10 decreases IL-6 production, and thereby indirectly affects the acute phase response, decreasing CRP, AGP, and ACT concentration in RA patients.Abbreviations ACT
-1-antichymotrypsin
- AGP
1-acid glycoprotein
- APP
acute phase protein
- CRP
C-reactive protein
- CSF
colony stimulating factor
- IFN
interferon
- IL
interleukin
- IMG
intramuscular gold
- MTX
methotrexate
- NSAID
non-steroidal anti-inflammatory drug
- RA
rheumatoid arthritis 相似文献
4.
Jacek Pika Micha Kwany Adam Filipkowski Ryszard Buczyski Mirosaw A. Karpierz Urszula A. Laudyn 《Materials》2022,15(21)
We study an optical device designed for converting the polarized Gaussian beam into an optical vortex of tunable polarization. The proposed device comprised a set of three specially prepared nematic liquid crystal cells and a nano-spherical phase plate fabricated from two types of glass nanotubes. This device generates a high-quality optical vortex possessing one of the multiple polarization states from the uniformly polarized input Gaussian beam. Its small size, simplicity of operation, and electrical steering can be easily integrated into the laboratory and industrial systems, making it a promising alternative to passive vortex retarders and spatial light modulators. 相似文献
5.
Ahsan Hameed Edyta Adamska-Patruno Joanna Godzien Przemyslaw Czajkowski Urszula Miksza Karolina Pietrowska Joanna Fiedorczuk Monika Moroz Witold Bauer Julia Sieminska Maria Grska Adam Jacek Krtowski Michal Ciborowski 《Nutrients》2022,14(20)
The relationship of high-carbohydrate (HC) meal intake to metabolic syndrome is still not fully explained. Metabolomics has the potential to indicate metabolic pathways altered by HC meals, which may improve our knowledge regarding the mechanisms by which HC meals may contribute to metabolic syndrome development. The fasting and postprandial metabolic response to HC or normo-carbohydrate (NC) meals with/without cinnamon + capsicum intake was evaluated using untargeted metabolomics and compared between normal-weight (NW) and overweight/obese (OW/OB) healthy men. Healthy male participants (age-matched) were divided into two groups (12 subjects per group). One was composed of men with normal weight (NW) and the other of men with overweight/obesity (OW/OB). On separate visits (with 2–3 week intervals), the participants received standardized HC or NC meals (89% or 45% carbohydrates, respectively). Fasting (0 min) and postprandial (30, 60, 120, 180 min) blood were collected for untargeted plasma metabolomics. Based on each metabolic feature’s intensity change in time, the area under the curve (AUC) was calculated. Obtained AUCs were analyzed using multivariate statistics. Several metabolic pathways were found dysregulated after an HC meal in people from the OW/OB group but not the NW group. The consumption of HC meals by people with overweight/obesity led to a substantial increase in AUC, mainly for metabolites belonging to phospholipids and fatty acid amides. The opposite was observed for selected sphingolipids. The intake of cinnamon and capsicum normalized the concentration of selected altered metabolites induced by the intake of HC meals. A HC meal may induce an unfavourable postprandial metabolic response in individuals with overweight/obesity, and such persons should avoid HC meals. 相似文献
6.
7.
Maciej Zacharski Alicja Tomaszek Liliana Kiczak Maciej Ugorski Jacek Bania Urszula Pasławska Ilona Rybinska Ewa Anita Jankowska Adrian Janiszewski Piotr Ponikowski 《Journal of cardiac failure》2021,27(6):682-692
BackgroundSteroid hormones play an important role in heart failure (HF) pathogenesis, and clinical data have revealed disordered steroidogenesis in male patients with HF. However, there is still a lack of studies on steroid hormones and their receptors during HF progression. Therefore, a porcine model of tachycardia-induced cardiomyopathy corresponding to HF was used to assess steroid hormone concentrations in serum and their nuclear receptor levels in heart tissue during the consecutive stages of HF.Methods and ResultsMale pigs underwent right ventricular pacing and developed a clinical picture of mild, moderate, or severe HF. Serum concentrations of dehydroepiandrosterone, testosterone, dihydrotestosterone, estradiol, aldosterone, and cortisol were assessed by enzyme-linked immunosorbent assay. Androgen receptor, estrogen receptor alpha, mineralocorticoid receptor, and glucocorticoid receptor messenger RNA levels in the left ventricle were determined by qPCR.The androgen level decreased in moderate and severe HF animals, while the corticosteroid level increased. The estradiol concentration remained stable. The quantitative real-time polymerase chain reaction revealed the downregulation of androgen receptor in consecutive stages of HF and increased expression of mineralocorticoid receptor messenger RNA under these conditions.ConclusionsIn the HF pig model, deteriorated catabolic/anabolic balance, manifested by upregulation of aldosterone and cortisol and downregulation of androgen signaling on the ligand level, was augmented by changes in steroid hormone receptor expression in the heart tissue. 相似文献
8.
9.
Grzegorz Lis Ewa Cichocka-Jarosz Urszula Jedynak-Wasowicz Edyta Glowacka 《Jornal brasileiro de pneumologia》2014,40(1):82-85
Plastic bronchitis (PB), although a rare cause of airway obstruction, has mortality
rates up to 50% in children after Fontan-type cardiac surgery. We present the case of
an 18-month-old female patient with PB following pneumonia. At 6 months of age, the
patient underwent the Glenn procedure due to functionally univentricular heart.
Fiberoptic bronchoscopy revealed complete blockage of the left bronchus by mucoid
casts. Pharmacotherapy consisted of glucocorticosteroids, azithromycin, and enalapril
maleate. The child also received nebulized 3% NaCl solution, which proved to be
beneficial. In children submitted to Fontan-type procedures, physicians must be alert
for PB, which can be triggered by respiratory tract infection. 相似文献
10.
Wróbel Andrzej Doboszewska Urszula Rechberger Ewa Rojek Karol Serefko Anna Poleszak Ewa Skalicka-Woźniak Krystyna Dudka Jarosław Wlaź Piotr 《Naunyn-Schmiedeberg's archives of pharmacology》2017,390(6):613-619
Naunyn-Schmiedeberg's Archives of Pharmacology - Hemorrhagic cystitis often develops in patients treated with cyclophosphamide (CYP). Studies have indicated that Rho kinase (ROCK) inhibitors... 相似文献