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排序方式: 共有170条查询结果,搜索用时 46 毫秒
1.
Spontaneous generation of functional osteoclasts from synovial fluid mononuclear cells as a model of inflammatory osteoclastogenesis 下载免费PDF全文
Stinne R. Greisen Halldór Bjarki Einarsson Malene Hvid Ellen‐Margrethe Hauge Bent Deleuran Tue Wenzel Kragstrup 《APMIS : acta pathologica, microbiologica, et immunologica Scandinavica》2015,123(9):779-786
In osteoimmunology, osteoclastogenesis is understood in the context of the immune system. Today, the in vitro model for osteoclastogenesis necessitates the addition of recombinant human receptor activator of nuclear factor kappa‐B ligand (RANKL) and macrophage colony‐stimulating factor (M‐CSF). The peripheral joints of patients with rheumatoid arthritis (RA) and spondyloarthritis (SpA) are characterized by an immune‐mediated inflammation that can lead to bone destruction. Here, we evaluate spontaneous in vitro osteoclastogenesis in cultures of synovial fluid mononuclear cells (SFMCs) activated only in vivo. SFMCs were isolated and cultured for 21 days at 0.5–1.0 × 106 cells/mL in culture medium. SFMCs and healthy control peripheral blood monocytes were cultured with RANKL and M‐CSF as controls. Tartrate‐resistant acid phosphatase (TRAP) positive multinucleated cells were found in the SFMC cultures after 21 days. These cells expressed the osteoclast genes calcitonin receptor, cathepsin K, and integrin β3, formed lacunae on dentin plates and secreted matrix metalloproteinase 9 (MMP9) and TRAP. Adding RANKL and M‐CSF potentiated this secretion. In conclusion, we show that SFMCs from inflamed peripheral joints can spontaneously develop into functionally active osteoclasts ex vivo. Our study provides a simple in vitro model for studying inflammatory osteoclastogenesis. 相似文献
2.
Daniel Mønsted Shabanzadeh Lars Tue Sørensen Torben Jørgensen 《Scandinavian journal of gastroenterology》2016,51(10):1239-1248
Objective: Only few determinants of gallstone formation have been identified in cohort studies. The aim was to identify further determinants for gallstones in a Danish cohort and to perform a meta-analysis of results from existing cohorts.Material and methods: Data from a cohort study was used. Gallstone incidence was assessed through repeated ultrasound examinations. Body mass index (BMI), blood pressure, self-rated health, lifestyle variables, blood lipids, and use of female sex hormones were measured at the baseline examination. Statistical analyses included logistic regression. Based on a prospective protocol, a systematic review of the literature was performed identifying all articles dealing with determinants of incident gallstones. Meta-analyses of comparable determinants were performed through fixed effect models.Results: Participants with no gallstones at baseline and with at least one re-examination were followed-up completely (mean 11.6 years, N?=?2848). The overall cumulative incidence of gallstones was 0.60% per year. Independent positive determinants for incident gallstones were age, female sex, non-high density lipoprotein (non-HDL) cholesterol, and gallbladder polyps. In addition, BMI was positively associated in men. The systematic review additionally identified associations for comorbidities, parity, and dietary factors. Meta-analysis confirmed the significant associations for incident gallstones and age, female sex, BMI, and non-HDL cholesterol. No significant associations were found for blood pressure, smoking, alcohol consumption, HDL cholesterol, or triglycerides in meta-analyses.Conclusions: Age, female sex, BMI, non-HDL cholesterol, and polyps are independent determinants for gallstone formation. Incident gallstones and the metabolic syndrome share common risk factors. More studies are needed for further exploration. 相似文献
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Summary This paper develops a specification test for functional form for models identified by a conditional moment restriction, including IV and GMM settings. The framework is one where the moment restriction is specified as a function of data, a finite‐dimensional parameter vector and a non‐parametric function (an infinite‐dimensional parameter vector). The null hypothesis is that the moment restriction does not depend on the non‐parametric function. The test is relatively easy to implement and its asymptotic distribution is known. The test performs well in simulation experiments. 相似文献
5.
René B. Svensson Tue Hassenkam Philip Hansen S. Peter Magnusson 《Journal of the Mechanical Behavior of Biomedical Materials》2010,3(1):112-115
Whole tendon and fibril bundles display viscoelastic behavior, but to the best of our knowledge this property has not been directly measured in single human tendon fibrils. In the present work an atomic force microscopy (AFM) approach was used for tensile testing of two human patellar tendon fibrils. Fibrils were obtained from intact human fascicles, without any pre-treatment besides frozen storage. In the dry state a single isolated fibril was anchored to a substrate using epoxy glue, and the end of the fibril was glued on to an AFM cantilever for tensile testing. In phosphate buffered saline, cyclic testing was performed in the pre-yield region at different strain rates, and the elastic response was determined by a stepwise stress relaxation test. The elastic stress-strain response corresponded to a second-order polynomial fit, while the viscous response showed a linear dependence on the strain. The slope of the viscous response showed a strain rate dependence corresponding to a power function of powers 0.242 and 0.168 for the two patellar tendon fibrils, respectively. In conclusion, the present work provides direct evidence of viscoelastic behavior at the single fibril level, which has not been previously measured. 相似文献
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Nghiem Nguyet Thu Truong Tuyet Mai Tran Th໋ Thu Trang Nguyen Anh Tuan Tran Chau Quyen Nguyen Lien Hanh Nguyen Huu Hoan Bui Thi Huong Lan Phung Thi Hau Ha Huy Tue Truong Viet Dung Ryohei Tsuji Yuta Watanabe Naoki Yamamoto Osamu Kanauchi 《Nutrients》2022,14(3)
Lactococcus lactis strain Plasma (LC-Plasma) is reported to have anti-viral effects via direct activation of plasmacytoid dendritic cells, which upregulate the production of type I and III interferons. A randomized, placebo-controlled, double-blind, parallel group study was designed for elementary schoolchildren, grades 1 to 3, in Vietnam. LC-Plasma or a control were administered to schoolchildren as a beverage (1.0 × 1011 count LC-Plasma/day/person). The primary endpoint was to determine the efficacy of LC-Plasma in reducing the cumulative days absent from school due to upper respiratory disease (URID) and gastrointestinal disease (GID), and the secondary endpoint was to evaluate the potency of LC-Plasma on URID/GID symptoms and general well-being scores. LC-Plasma intake significantly reduced the cumulative days absent from school due to URID/GID (Odds ratio (OR) = 0.57, p = 0.004) and URID alone (OR = 0.56, p = 0.005); LC-Plasma also significantly reduced the number of cumulative fever positive days during the first 4 weeks of intervention (OR = 0.58, p = 0.001) and cumulative days with diarrhea during the last 4 weeks of the intervention period (OR = 0.78, p = 0.01). The number of positive general wellbeing days was significantly improved in the LC-Plasma group compared with the control throughout the intervention period (OR = 0.93, 0.93, p = 0.03, 0.04 in the first and last 4 weeks of the intervention, respectively). These data suggest that LC-Plasma seems to improve the health condition of elementary schoolchildren and reduces school absenteeism due to infectious disease, especially URID. 相似文献
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9.
Smoking Is a Risk Factor for Recurrence of Groin Hernia 总被引:15,自引:0,他引:15
Sorensen LT Friis E Jorgensen T Vennits B Andersen BR Rasmussen GI Kjaergaard J 《World journal of surgery》2002,26(4):397-400
Studies of connective tissue from
patients with inguinal hernia have shown that smoking may be associated
with hernia formation due to a defective connective tissue metabolism.
Whether smoking is a risk factor for recurrence, too, was examined in
this study. From December 1990 through December 1995, 649 patients
underwent hernia repair as open sutured repair (Cooper ligament or
abdominal ring repair) or as open mesh repair. Five hundred forty-four
eligible patients were evaluated for recurrence 2 years
postoperatively. Association between recurrence and 17 patient-,
disease-, and intraoperative variables were analyzed by multiple
logistic regression. The results showed that smoking was significantly
and independently associated with recurrence compared to nonsmoking
[odds ratio (OR = 2.22; 95% confidence interval (95% CI) =
1.19–4.15)]. Open sutured repair compared to open mesh repair was the
most significant predictor for recurrence (OR = 7.23; 95% CI
= 3.01–17.37). Surprisingly, local anesthesia was associated with a
higher risk of recurrence compared to general anesthesia (OR =
2.44; 95% CI = 1.19–5.09). Potential confounders and other risk
factors for hernia recurrence such as age, alcohol consumption,
previous surgery, and anatomical characteristics of the hernia were
adjusted for in the analysis. In conclusion, smoking is an important
risk factor for recurrence of groin hernia, presumably due to an
abnormal connective tissue metabolism in smokers. 相似文献
10.
Nanoscale structural analyses of biomineralized materials can frequently help elucidate important structure-function relationships in these complex organic-inorganic composites. Atomic force microscope (AFM) imaging of the exterior surface of trabecular bone reveals a densely woven structure of collagen fibrils, banded with a 67-nm periodicity, and densely packed mineral plates. The mineral plates on the collagen fibrils overlap and exhibit a large range of plate diameters from 30 to 200 nm. On the collagen fibrils, small nodular features, spaced 20-30 nm, run perpendicular to the fibrils. In some cases, these nodules are also seen on filaments extending between collagen fibrils. We hypothesize that these protrusions are noncollagenous proteins such as proteoglycans and may have collapsed into compact structures when the sample was dried. AFM images of pristine fractured surfaces reveal a dense array of mineral plates. In a few isolated locations, short sections of bare collagen fibrils are visible. In other regions, the existence of the underlying collagen fibrils can be inferred from the linear patterns of the mineral plates. Fractured samples, rinsed to remove mineral plates, reveal separated collagen fibrils on the fractured surfaces. These fibrils are often covered with protrusions similar to those observed on the exterior surfaces but are less organized. In addition, as on the exterior surfaces, there are sometimes small filaments extending between neighboring collagen fibrils. These studies provide important insights into the nanostructured architecture of this complex biocomposite. 相似文献