全文获取类型
收费全文 | 378篇 |
免费 | 1篇 |
专业分类
耳鼻咽喉 | 6篇 |
儿科学 | 16篇 |
妇产科学 | 5篇 |
基础医学 | 131篇 |
口腔科学 | 2篇 |
临床医学 | 54篇 |
内科学 | 31篇 |
皮肤病学 | 4篇 |
神经病学 | 12篇 |
特种医学 | 9篇 |
外科学 | 31篇 |
综合类 | 2篇 |
预防医学 | 26篇 |
药学 | 34篇 |
肿瘤学 | 16篇 |
出版年
2022年 | 4篇 |
2021年 | 5篇 |
2016年 | 4篇 |
2015年 | 3篇 |
2014年 | 4篇 |
2013年 | 9篇 |
2012年 | 5篇 |
2011年 | 8篇 |
2010年 | 10篇 |
2009年 | 9篇 |
2008年 | 13篇 |
2007年 | 12篇 |
2006年 | 10篇 |
2005年 | 6篇 |
2004年 | 7篇 |
2003年 | 4篇 |
2002年 | 9篇 |
2001年 | 7篇 |
2000年 | 11篇 |
1999年 | 12篇 |
1998年 | 4篇 |
1993年 | 6篇 |
1992年 | 12篇 |
1991年 | 6篇 |
1990年 | 14篇 |
1989年 | 12篇 |
1988年 | 14篇 |
1987年 | 9篇 |
1986年 | 13篇 |
1985年 | 15篇 |
1984年 | 7篇 |
1983年 | 4篇 |
1982年 | 7篇 |
1981年 | 3篇 |
1979年 | 7篇 |
1978年 | 7篇 |
1976年 | 6篇 |
1975年 | 5篇 |
1974年 | 9篇 |
1973年 | 9篇 |
1972年 | 5篇 |
1971年 | 5篇 |
1970年 | 4篇 |
1969年 | 3篇 |
1968年 | 7篇 |
1967年 | 3篇 |
1965年 | 7篇 |
1958年 | 2篇 |
1957年 | 2篇 |
1956年 | 2篇 |
排序方式: 共有379条查询结果,搜索用时 15 毫秒
1.
2.
G P Matve?kov V I Levin I P Titova E S Kaliia A N Solove? L G Atamanenko 《Terapevticheski? arkhiv》1992,64(2):89-93
Soluble antigen HLA-B27 was assayed in blood serum of 30 patients afflicted with ankylosing spondyloarthritis (AS) and 25 healthy subjects having HLA-B27 (B27+) antigen in the phenotype. As compared to B27+ healthy subjects, B27+ patients with AS demonstrated a significantly higher level of soluble HLA-B27 antigen. A substance whose functional activity was found to be identical to that of soluble HLA-B27 antigen in blood serum of B27- patients with AS was discovered for the first time. It was absent from B27- healthy subjects. In addition to the identification of the fixed forms of soluble HLA-B27 antigen, the assay of this antigen in blood serum enhances the potentialities of laboratory diagnosis of AS, which plays an important role at the early disease stages. 相似文献
3.
Evaluation of three substitutes for Percoll in sperm isolation by density gradient centrifugation 总被引:9,自引:4,他引:9
Silane-coated silica particle solutions (ISolate(TM) and PureSperm)TM)) and
iodixanol (OptiPrep(TM)) were compared to polyvinylpyrrolidone (PVP)-coated
silica particles (Percoll(TM)) in their efficacy to recover spermatozoa by
gradient centrifugation for use in assisted reproductive procedures.
Efficacy was assessed in terms of percentages of sperm recovery, sperm
vitality and motility, normal sperm morphology and normal sperm chromatin
condensation. No significant difference was found in the recovery of
spermatozoa for men with both normal sperm counts and oligozoospermia,
between PVP-coated and silane-coated particle solutions. Iodixanol had
significantly lower sperm recovery compared to the other products. Sperm
vitality, progressive motility, normal morphology and normal chromatin
condensation did not differ significantly between any of the sperm
isolation products.
相似文献
4.
Mechanical or thermal injury to one of a pair of parabiotic rats with a crossed circulation but separate innervation was followed by the development of a state resembling shock in the other partner. Microcirculatory changes characteristic of either traumatic or burn shock developed in its mesentery under these circumstances. The results are interpreted as evidence in support of a humoral rather than a nervous mechanism of the microcirculatory disturbances in these types of shock.Laboratory of Pathophysiology of Extremal States, Research Institute of General Pathological Physiology, Academy of Medical Sciences of the USSR, Moscow. (Presented by Academician of the Academy of Medical Sciences of the USSR N. A. Fedorov.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 86, No. 10, pp. 416–418, October, 1978. 相似文献
5.
Wendy Lo Thomas Bravo Vikram Jadhav Elena Titova John H. Zhang Jiping Tang 《Neuroscience letters》2007
Neurosurgical procedures can result in brain injury by various means including direct trauma, hemorrhage, retractor stretch, and electrocautery. This surgically-induced brain injury (SBI) can cause post-operative complications such as brain edema. By creating a mouse model of SBI, we tested whether NADPH oxidase, an important reactive oxygen species producing enzyme, is involved in SBI using transgenic mice lacking gp91phox subunit of NADPH oxidase (gp91phox KO) and apocynin, a specific inhibitor of NADPH oxidase. Neurological function and brain edema were evaluated at 24 h post-SBI in gp91phox KO and wild-type littermates grouped into SBI and sham-surgery groups. Alternatively, mice were grouped into vehicle- and apocynin-treated (5 mg/kg, i.p. 30 min before SBI) groups. Oxidative stress indicated by lipid peroxidation (LPO) was measured at 3 and 24 h post-SBI. The gp91phox KO mice, but not the apocynin-treated mice showed significantly improved neurological scores. Brain edema was observed in both gp91phox KO and wild-type groups after SBI; however, there was no significant difference between these two groups. Brain edema was also not affected by apocynin-pretreatment. LPO levels were significantly higher in SBI group in both gp91phox KO and wild-type groups as compared to sham group. A trend, although without statistical significance, was noted towards attenuation of LPO in the gp91phox KO animals as compared to wild-type group. LPO levels were significantly attenuated at 3 h post-SBI by apocynin-pretreatment but not at 24 h post-SBI. These results suggest that chronic and acute inhibition of NADPH oxidase activity does not reduce brain edema after SBI. Long-term inhibition of NADPH oxidase, however improves neurological functions after SBI. 相似文献
6.
Protective cytotoxic T lymphocyte responses against paramyxoviruses induced by epitope-based DNA vaccines: involvement of IFN-gamma 总被引:1,自引:0,他引:1
Hsu SC; Obeid OE; Collins M; Iqbal M; Chargelegue D; Steward MW 《International immunology》1998,10(10):1441-1447
Plasmid DNA vectors have been constructed with minigenes encoding a single
cytotoxic T lymphocyte (CTL) epitope from either the M2 protein of
respiratory syncytial virus (RSV) or from the nucleoprotein of measles
virus (MV) with or without a signal sequence (also called secretory or
leader sequence). Following intradermal immunization, plasmids in which the
CTL epitopes were expressed in-frame with the signal sequence were more
effective at inducing peptide- and virus- specific CTL responses than
plasmids expressing CTL epitopes without the signal sequence. This
immunization resulted in protection against MV-induced encephalitis and a
significant reduction in viral load following RSV challenge. The reduction
of viral load following RSV challenge was abrogated by prior injection with
anti-IFN-gamma antibodies. These results highlight the ability of
epitope-based DNA immunization to induce protective immune responses to
well-defined epitopes and indicate the potential of this approach for the
development of vaccines against infectious diseases.
相似文献
7.
8.
Rundek T Di Tullio MR Sciacca RR Titova IV Mohr JP Homma S Sacco RL 《Stroke; a journal of cerebral circulation》1999,30(12):2683-2686
BACKGROUND AND PURPOSE: Aortic arch atheromas (AAs) have been shown to be a risk factor for ischemic stroke (IS) in the elderly because of their potential for cerebral embolization. However, the association between AAs and the presence of cerebral microemboli has not been clearly established. The aim of this study was to determine whether large AAs are associated with an increased frequency of high-intensity transient signals (HITS) in elderly patients with IS. METHODS: We performed bitemporal simultaneous HITS monitoring of both middle cerebral arteries in 62 consecutive elderly patients with acute IS (mean age 72.5+/-8.8 years, 65% men). In 16 patients, one or both temporal windows were inadequate; therefore, the analysis of HITS was performed in the remaining 46 patients. All patients underwent omniplane transesophageal echocardiography (TEE), and they had no significant extracranial or intracranial artery disease and no cardiac prosthetic valves. Large AA was defined as > or = 4 mm in thickness. Complex AA was defined as ulcerated or mobile, regardless of plaque thickness. HITS monitoring was performed within 24 hours of TEE and analyzed by an experienced neurologist-sonographer blinded to TEE findings. A 9-dB threshold was chosen to discriminate HITS from background Doppler signal. The HITS counts in the left and in the right middle cerebral arteries were added and reported as a total number of HITS in 30 minutes. RESULTS: HITS were detected in 14 (78%) of 18 patients with large AAs versus 8 (29%) of 28 patients with no or small AAs (odds ratio [OR] 8.8, 95% CI 2.2 to 34.8; P=0. 001). The association was also present in 27 patients with no other cardiac embolic sources, such as atrial fibrillation, patent foramen ovale, spontaneous echo contrast, and thrombus (7 of 10 patients with large AAs versus 3 of 17 patients with small or no AA; OR 10.9, 95% CI 1.7 to 68.5; P=0.013). Complex AAs were associated with a higher frequency of HITS than were noncomplex AAs (6 of 6 patients with complex AAs versus 15 of 39 patients with noncomplex AAs; OR 2. 6, 95% CI 1.7 to 3.9; P=0.005). CONCLUSIONS: HITS are significantly associated with large AAs in elderly stroke patients. This observation may support the causal role of large AAs in IS. 相似文献
9.
Al. A. Sergeev A. S. Kabanov L. E. Bulychev Ar. A. Sergeev O. V. Pyankov S. A. Bodnev D. O. Galahova A. S. Zamedyanskaya K. A. Titova A. G. Glotov O. S. Taranov V. V. Omigov L. N. Shishkina A. P. Agafonov A. N. Sergeev 《Transboundary and Emerging Diseases》2016,63(5):e419-e430
As a result of the conducted experimental studies on intranasal challenge of ICR mice, rabbits and miniature pigs (even in the maximum variant) with the doses of 4.0–5.5 lg PFU of monkeypox virus (MPXV), some clinical signs such as purulent conjunctivitis, blepharitis and ruffled fur were found only in mice. The 50% infective dose (CID50) of MPXV for these animals estimated by the presence of external clinical signs was 4.8 lg PFU, and LID50 estimated by the virus presence in the lungs of mice 7 days post‐infection taking into account its 10% application in the animal respiratory tract was 1.4 lg PFU. When studying the dynamics of MPXV propagation in mice challenged intranasally with 25 LID50 of MPXV, the maximum pathogen accumulation was revealed in nasal cavity, lungs and brain: 5.7 ± 0.1, 5.5 ± 0.1 and 5.3 ± 0.3 lg PFU/ml, respectively. The pathomorphological examination of these animals revealed the presence and replication of the pathogen in the traditional primary target cells for MPXV (mononuclear phagocyte system cells and respiratory tract epitheliocytes) as well as in some other types of cells (endothelial cells, reticular cells, connective tissue cells). Our use of these animals to assess the antiviral efficacy of some drugs demonstrated the agreement of the results (a significant positive effect of NIOCH‐14 and ST‐246) with those described in scientific literature, which opens up the prospects of using ICR mice as animal models for monkeypox to develop preventive antismallpox drugs. 相似文献
10.
Tobias I Ndubuisi Ezejiofor Anthonet N Ezejiofor Orish E Orisakwe Hariet C Nwigwe Ferdinand OU Osuala Moses OE Iwuala 《Journal of occupational medicine and toxicology (London, England)》2014,9(1):1-14